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Dibutyrate derivatization of monoacylglycerols for the resolution of regioisomers of oleic, petroselinic, and cis-vaccenic acids

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Lipids

Abstract

Dibutyrate derivatives of monoacylglycerols of oleic, petroselinic, and cis-vaccenic acids were prepared by diesterification of monoacylglycerols with n-butyryl chloride. The resulting triacylglycerols were analyzed by gas chromatography (GC) with a 65% phenyl methyl silicone capillary column and separated on the basis of both fatty acid composition and regiospecific position. The petroselinic acid derivatives eluted first, followed sequentially by the oleic and cis-vaccenic acid derivatives, with the sn−2 positional isomer eluting before the sn−1(3) isomer in each case. Separation of the peaks was almost baseline between petroselinic and oleic acids as well as between oleic and cis-vaccenic acids. To assess the accuracy of the method, mixtures of triolein, tripetroselinin, and tri-cis-vaccenin in various known proportions were partially deacylated with the use of ethyl magnesium bromide and derivatized and analyzed as above. The results showed that this method compares favorably to the existing methods for analysis of oleic, petroselinic, and cis-vaccenic fatty acids by GC with respect to peak separation and accuracy, and it also provides information on the regiospecific distribution of the fatty acids. The method was applied to basil (Ocimum basilicum) and coriander (Coriandrum sativum) seed oils. cis-Vaccenic, oleic, and linoleic acids were mainly distributed at the sn−2 position in basil seed oil, and higher proportions of linolenic, palmitic, and stearic acids were distributed at the sn−1(3) position than at the sn−2 position. In coriander seed oil, petroselinic acid was mainly distributed at the sn−1(3) position, and both oleic and linoleic acids were mostly located at the sn−2 position, whereas palmitic, stearic, and cis-vaccenic acids were located only at the sn−1(3) position.

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Abbreviations

GC:

gas chromatography

HPLC:

high-performance liquid chromatography

TAG:

triacylglycerol

TLC:

thin-layer chromatography

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Author information

Authors and Affiliations

  1. Department of Food Science and Nutrition, Université Laval, G1K 7P4, Sainte-Foy, Québec, Canada

    Frédéric Destaillats, Joseph Arul & Paul Angers

  2. Dairy Research Center (STELA), Université Laval, G1K 7P4, Sainte-Foy, Québec, Canada

    Frédéric Destaillats, Joseph Arul & Paul Angers

  3. Department of Plant Science, Rutgers University, 08901, New Brunswick, New Jersey

    James E. Simon

  4. INRA-UNL, 21065, Dijon Cedex, France

    Robert L. Wolff

Authors
  1. Frédéric Destaillats
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  2. Joseph Arul
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  3. James E. Simon
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  4. Robert L. Wolff
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  5. Paul Angers
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Correspondence to Paul Angers.

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Destaillats, F., Arul, J., Simon, J.E. et al. Dibutyrate derivatization of monoacylglycerols for the resolution of regioisomers of oleic, petroselinic, and cis-vaccenic acids. Lipids 37, 111–116 (2002). https://doi.org/10.1007/s11745-002-0870-x

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  • DOI: https://doi.org/10.1007/s11745-002-0870-x

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