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Regiospecific analysis of fractions of bovine milk fat triacylglycerols with the same partition number

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Lipids

Abstract

Bovine milk fat was fractionated using preparative reversed-phase high-performance liquid chromatography. The conditions consisted of two successive linear gradients of acetonitrile and tert-butylmethylether, followed by a final isocratic mixture of the two eluants, leading to triacylglycerols grouped by their partition number (PN). Fractions corresponding to partition numbers 32 to 50 were isolated and analyzed for fatty acid distribution between sn-1,3 and sn-2 positions by Grignard degradation. Results showed that the fatty acid distribution in milk fat triacylglycerols is nonrandom. The distribution of short-chain fatty acids, stearic (predominantly at sn-1,3 position) and palmitic (predominantly sn-2 position), did not change with triacylglycerol size. Medium-chain fatty acids were predominantly located at sn-2 position, but their proportion at this position decreased with triacylglycerol size. Oleic acid distribution was also size-dependent in that it was located in high proportions at sn-2 position in smaller triacylglycerols and vice versa. Results also showed that the sn-2 position was more unsaturated than sn-1,3 position in the PN range from 32 to 40, but it was more saturated in triacylglycerols with higher PN.

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Abbreviations

ACN:

acyl carbon number

AN:

acetonitrile

HPLC:

high-performance liquid chromatography

MTBE:

methyl-tert-butyl ether

PN:

partition number

TLC:

thin-layer chromatography

UV:

ultraviolet

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Authors and Affiliations

  1. Dairy Research Center (STELA), and Department of Food Science and Nutrition, Université Laval, Pavillon Puel Comtois, G1K 7P4, Sainte-Foy, Québec, Canada

    Paul Angers, Édith Tousignant, Armand Boudreau & Joseph Arul

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  1. Paul Angers
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  2. Édith Tousignant
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  3. Armand Boudreau
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  4. Joseph Arul
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Correspondence to Joseph Arul.

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Angers, P., Tousignant, É., Boudreau, A. et al. Regiospecific analysis of fractions of bovine milk fat triacylglycerols with the same partition number. Lipids 33, 1195–1201 (1998). https://doi.org/10.1007/s11745-998-0323-6

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  • DOI: https://doi.org/10.1007/s11745-998-0323-6

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