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The effect of phosphate deficiency on membrane phospholipid composition of bean (Phaseolus vulgaris L.) roots

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Abstract

Plasma membranes were isolated from roots of bean (Phaseolus vulgaris L.) plants cultured on phosphate sufficient or phosphate deficient medium. The phospholipid composition of plasma membranes was analyzed and compared with that of the microsomal fraction. Phosphate deficiency had no influence on lipid/protein ratio in microsomal as well as plasma membrane fraction. In phosphate deficient roots phospholipid content was lower in the plasma membrane, but did not change in the microsomal fraction. Phosphatidylcholine and phosphatidylethanolamine were two major phospholipids in plasmalemma and microsomal membranes (80 % of the total). After two weeks of phosphate starvation a considerable decrease (about 50 %) in phosphatidylcholine and phosphatidylethanolamine in microsomal membranes was observed. The decline in two major phospholipids was accompanied by an increase in phosphatidic acid and lysophosphatidylcholine content. The effect of alterations in plasma membrane phospholipids on membrane function e.g. nitrate uptake is discussed.

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Abbreviations

LPC:

lysophosphatidylcholine

+P:

control plants

-P:

phosphate deficient plants

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PG:

phosphatidylglycerol

PI:

phosphatidylinositol

PS:

phosphatidylserine

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Authors and Affiliations

  1. Institute of Experimental Plant Biology, University of Warsaw, Pawińskiego 5A, 02-106, Warsaw, Poland

    Agnieszka Gniazdowska, Bożena Szal & Anna M. Rychter

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  1. Agnieszka Gniazdowska
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  2. Bożena Szal
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  3. Anna M. Rychter
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Gniazdowska, A., Szal, B. & Rychter, A.M. The effect of phosphate deficiency on membrane phospholipid composition of bean (Phaseolus vulgaris L.) roots. Acta Physiol Plant 21, 263–269 (1999). https://doi.org/10.1007/s11738-999-0041-9

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  • DOI: https://doi.org/10.1007/s11738-999-0041-9

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