Abstract
Short-term treatment of potato tuber (Solanum tuberosum L.) discs with CdCl2 changed glutathione reductase (GR) activity depending on cadmium ions concentrations, kind of tuber and time of incubation. The increase of GR activity at 10 and 100 µmol·dcm−3 of CdCl2 solutions was marked in less resistant tissues of cv. Bintje after 24 hrs, and was slight in more resistant tissues of cv. Bzura after 72 hrs. At 1 mmol·dcm−3 concentration of CdCl2 rapid and total inactivation in both kind of tissues was observed, which disappeared after a few days. However this elevation was faster in more resistant tissues. These inhibition effects come from the inactivation process of GR by cadmium. The values of KI for cadmium and KM for GSSG of GR from potato tuber tissues indicated that enzyme from more resistant tissues possessed lower affinity to toxic metal and higher affinity to substrate.
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Stroiński, A., Kubiś, J. & Zielezińska, M. Effect of cadmium on glutathione reductase in potato tubers. Acta Physiol Plant 21, 201–207 (1999). https://doi.org/10.1007/s11738-999-0033-9
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DOI: https://doi.org/10.1007/s11738-999-0033-9