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Heterologous expression of Sesuvium portulacastrum SOS-related genes confer salt tolerance in yeast

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Abstract

Growth and development of plants are greatly affected by salinity. The salt overly sensitive (SOS) pathway plays a vital role in plants responding to salt stress, and has been reported to contain three components: SOS3 (calcineurin B-like protein 4, CBL4), SOS2 (CBL-interacting protein kinase 24, CIPK24) and SOS1. Our previous study demonstrated that transgenic yeast and Arabidopsis could tolerate salt better when expressed with the cell membrane Na+/H+ antiporter SOS1 from the halophyte Sesuvium portulacastrum. Here, a new CIPK gene (SpCIPK8) and CBL gene (SpCBL10) have been isolated from S. portulacastrum. The expression of SpCIPK8 and SpCBL10 was induced by salinity in roots of S. portulacastrum. An interaction between SpCBL10 and SpCIPK8 was demonstrated in yeast two-hybrid assays. Subsequent analysis found that SpCBL10 could bind the C-terminus of SpCIPK8. Yeast co-expressing SpSOS1, SpCIPK8 and SpCBL10 genes grew better and accumulated more potassium (K+) and less sodium (Na+) under salt stress than yeast that expressed only one or two of these genes, indicating that Na+ was excluded from the cells. Furthermore, we found that the SpCBL10/SpCIPK8 complex regulates the cell membrane Na+/H+ antiporter SpSOS1 to enhance yeast salt tolerance by binding the two serine residues at amino acid positions 1144 and 1146 in the conserved DSPS motif at the C-terminus of SpSOS1. Future studies of the SOS pathway will be greatly aided by these results, which suggest some candidate genes for improving plant salt tolerance.

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Acknowledgements

This research was supported by the National Key Research and Development Program of China (2018YFE0207203-2), the Education Department of Hainan Province (Hnky2021-19), Hainan Provincial Natural Science Foundation of China (318QN189), the National Natural Science Foundation of China (31660253), and the Startup funding from Hainan University (KYQD(ZR)1845).

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  1. Yang Zhou and Yunfeng Zhu have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province/Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops/School of Horticulture, Hainan University, Haikou, 570228, China

    Yang Zhou, Yunfeng Zhu, Tingting Zhang, Yuxin Li, Yuqian Kang, Jian Wang & Xingyu Jiang

  2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou, 571101, China

    Yang Zhou & Jianchun Guo

  3. State Key Laboratory of Cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, 45500, China

    Wei Li

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11738_2023_3518_MOESM1_ESM.doc

Table S1 Primers sequences used in this study. Indicated are information about the primer names and primer sequences. Restriction sites are underlined. Table S2 Percentage of homology between SpCBL10 and Arabidopsis CBL10 proteins. Table S3. Percentage of homology between SpCIPK8 and Arabidopsis CIPK proteins (DOC 84 KB)

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Zhou, Y., Zhu, Y., Li, W. et al. Heterologous expression of Sesuvium portulacastrum SOS-related genes confer salt tolerance in yeast. Acta Physiol Plant 45, 58 (2023). https://doi.org/10.1007/s11738-023-03518-7

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