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Analysis of genetic diversity among medicinal therapist Trigonella foenum-graecum L. genotypes through RAPD and SSR markers

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Abstract

Trigonella is recognized as a medicinal therapist throughout the globe due to its multifaceted rare medicinal properties. It is indigenous from Iran to Northern India but has gained global acceptance towards cultivation and consumption for its yellow-to-amber colored seed which substantially contributes to food, pharmaceutical, nutraceutical and cosmetic industry. Genetic diversity serves as an excellent tool for developing improved crop varieties with breeder preferred traits. Unfortunately, very little information available on variability existing in commercial Trigonella genotypes considerably impedes the crop improvement. In this study, ninety Trigonella genotypes belonging to most productive North Indian states were subjected to multilocus genotyping using RAPD (49) and SSR (13) primers and detected an average of 55.60 and 50.16% polymorphism, respectively. The percentage polymorphism range (RAPD, 16.7–90.90; SSR, 33.30–66.66) average band informativeness (RAPD, 0.182–0.85; SSR, 0.21–0.91) and resolving power (RAPD, 0.95–9.984; SSR, 1.68–7.28) obtained revealed the wide range of diversity prevailing among these genotypes. Hierarchical clustering of genotypes in nine different clusters showed Trigonella’s genetic variability has wide genetic distribution across different agro-climatic zones. No consistency was observed while grouping Trigonella varieties based on eco-geographical region. Eventually, knowledge of these genetic differences significantly contributes in designing intra-specific crosses with potential interest to spice breeding programs. To the best of our knowledge, this is the first report of genetic diversity using SSR molecular markers in Trigonella foenum-graecum L.

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Acknowledgements

This work was supported by University Grants Commission, New Delhi, India as a Major Research Project sanctioned to Professor Ashok Chaudhury vide letter No. F.37-183/2009 (SR) dated 12 January 2010. Ms. Annu Sindhu duly acknowledges UGC, New Delhi for the financial assistance as a Project Fellow.

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  1. Annu Sindhu

    Present address: , 8/21-29, Trickey Avenue, Sydenham, Victoria, VIC, 3037, Australia

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  1. Plant Molecular Biology Laboratory, Department of Bio and Nano Technology, Bio and Nano Technology Centre, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India

    Annu Sindhu & Ashok Chaudhury

  2. Department of Vegetable Sciences, CCS Haryana Agricultural University, Hisar, 125004, Haryana, India

    Suresh Kumar Tehlan

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Correspondence to Ashok Chaudhury.

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Sindhu, A., Tehlan, S.K. & Chaudhury, A. Analysis of genetic diversity among medicinal therapist Trigonella foenum-graecum L. genotypes through RAPD and SSR markers. Acta Physiol Plant 39, 100 (2017). https://doi.org/10.1007/s11738-017-2395-8

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