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Methyl jasmonate-dependent senescence of cotyledons in Ipomoea nil

Abstract

Jasmonic acid methyl ester (JAMe) has been recently shown to play a crucial role in many physiological processes. In this paper, we focused on cotyledon senescence in Ipomoea nil and revealed that JAMe and darkness are the main factors stimulating the process examined. What is more, we showed that mefenamic acid (a jasmonate biosynthesis inhibitor) reverses the stimulatory effect of darkness on senescence. In plants growing under dark conditions, stimulation of JASMONIC ACID CARBOXYL METHYLTRANSFERASE (InJMT) expression and, consequently, an increase in JAMe content, have been observed. In turn, the level of jasmonic acid (JA) gradually decreased. Moreover, dark-grown seedlings demonstrated a lower PSII functional activity and a reduced chlorophyll content and autofluorescence. All of these data suggest that JAMe is a signal molecule controlling the senescence of cotyledons in I. nil.

Introduction

The senescence of cotyledons is the final stage of their development. This process is an integrated response of these organs to age information and other environmental signals, both internal and external, such as stresses of salinity, nutrient limitation or darkness (Bouchard and Yamasaki 2008; Corpas et al. 2009; Du et al. 2014; Ma et al. 2008; Neill et al. 2008; Xuan et al. 2010; Zhao et al. 2009). Although there is no single universal mechanism for regulating senescence, some characteristic stages of that process can be distinguished. Initially, thylakoid membrane structures are damaged, the photosynthetic rate drops as a result of chlorophyll degradation, and the catabolic process stops (Pružinská et al. 2005; Troncoso-Poncea et al. 2013; Wrischer et al. 2009; Zhang et al. 2010). Subsequently, the ER, ribosomes, proteins, lipids and nucleic acids are decomposed. In the final stage of cotyledon senescence, the mitochondria are degraded, oxidative phosphorylation intensity is decreased, and the tonoplast and nuclear membrane are interrupted, all of which lead to irreversible changes in the structures of the cytoplasm and the nucleus, as well as to the accumulation of secondary metabolites (Lim et al. 2007; Peterman and Siedow 1985). Although cotyledon senescence has been studied extensively (Ananieva et al. 2008a, b; Kanazawa et al. 2000; Krul 1974; McKersie et al. 1987; Peterman and Siedow 1985; Rukes and Mulkey 1993; Watanabe et al. 1994), its underlying regulatory network is unclear in comparison to leaf senescence (Lim et al. 2007). Numerous studies have shown that leaf senescence correlates with a progressive decline in the photosynthetic rate, which is accompanied by Rubisco and chlorophyll a/b binding protein (CAB) degradation (Avice and Etienne 2014; Gombert et al. 2006; Lu et al. 2002; Van der Graaff et al. 2006). There are crucial photosynthetic parameters commonly known as senescence physiological markers e.g., chlorophyll content, rate of photosynthesis (Gan 2004). In addition, the transcriptional activity of senescence-associated (SAGs) genes increased in leaves before or during senescence in different species (Breeze et al. 2011; Buchanan-Wollaston et al. 2005). Many SAGs are similar to genes probably involved in the breakdown and mobilization of nutrients, such as proteases, RNases, or glutamine synthetases (Lin et al. 2015). An essential role in coordinating anatomical, physiological and biochemical changes accompanying senescence is attributed to plant hormones, such as cytokinin, ethylene, abscisic acid, auxin and jasmonates (JAs) (Ananieva et al. 2008a; Ellis et al. 2005; Hung and Kao 2004; Jing et al. 2008; Okushima et al. 2005). Hormonal pathways are involved in cotyledon senescence—process that may be age-controlled or stimulated by plant responses to stresses. Such hormones may also affect the synthesis and signaling pathways of other to eventually trigger the expression of stress-responsive genes, which in turn influences cotyledon senescence (Lim et al. 2007).

Lipid-derived JAs arise from the enzymatic oxygenation of 18- and 16-carbon triunsaturated fatty acids. The biosynthesis of these hormones encompasses three cellular compartments: chloroplast, peroxisome and cytosol. The first stage occurs in the chloroplasts, where, thanks to the activity of 13-LOX (lipoxygenase), AOS (allene oxide synthase) and AOC (allene oxide cyclase), the 18:3 or 16:3 acid is oxidized and OPDA (12-oxophytodienoic acid) or dnOPDA (dinor-OPDA) is formed, respectively. Both compounds are transported into peroxisomes and with the participation of OPR3 (12-oxophytodienoate reductase), ACX (acyl-CoA oxidase), MFP (multifunctional protein) and KAT (l-3-ketoacyl-CoA thiolase) are first reduced, and then β-oxidized, which ends in the formation of the (+)-7-iso-jasmonic acid (JA) in the cytosol (Wasternack and Kombrink 2010). Then, JA can be metabolized by esterification into methyl (+)-7-iso-jasmonate (JAMe) or by amino acid conjugation to form (+)-7-iso-jasmonoyl-isoleucine (JA-Ile). The second is considered as the active form of the hormone. In addition, JA can be hydroxylated, sulfated, as well as glucosylated (Acosta and Farmer 2010).

The use of molecular biology techniques in research on the JA signal transduction pathway and regulation of its metabolism has led to the discovery that JA derivatives can be also biologically active in many physiological processes. Studies performed in many plant species show that JAMe, formed in the reaction catalyzed by JASMONIC ACID CARBOXYL METHYLTRANSFERASE (JMT), regulates the sprouting of seeds, participates in proper flower morphogenesis and flower induction, and is involved in responses to stress, which also includes promoting effect on leaf senescence (Ananieva et al. 2007; Kęsy et al. 2011; Maciejewska and Kopcewicz 2002; Maciejewska et al. 2004; Norastehnia et al. 2007; Wang 1998; Wilson et al. 2011).

In our former investigation, we showed that JAMe is an inhibitor of photoperiodic flower induction in model short-day plant (SDP) Ipomoea nil, in which the cotyledons are responsible for the perception of light stimuli (Kęsy et al. 2011; Maciejewska and Kopcewicz 2002; Maciejewska et al. 2004). Bearing in mind the involvement of JAMe in the control of many processes, we conducted physiological experiments on the efficiency of PSII, changes in the jasmonate level, chlorophyll content and autofluorescence. What is more, we performed identification and expression analyses of the InJMT gene participating in JA metabolism accompanying cotyledon senescence.

Materials and methods

Plant material

We prepared plant material (I. nil, Chois cv. Violet; Marutane Seed Co., Kyoto, Japan) according to Frankowski et al. (2009). The seedlings were grown in phytotrones under the conditions described by Wilmowicz et al. (2008). On 6th day of cultivation, half of the seedlings were exposed to darkness (D), whereas the remainder to continuous light (CL). The plants were watered with identical amounts of tap water every 2 days (Supplementary Fig. 1).

Plants growing in darkness were treated with mefenamic acid (MEF), a jasmonate biosynthesis inhibitor, at a concentration of 100 μM in 0.05 % Tween 20 (v/v), whereas plants cultivated under CL were treated with JA or JAMe at a concentration of 100 μM in 0.05 % Tween 20 (v/v). The control plants were treated with 0.05 % Tween 20 (v/v). All solutions were applied by small, soft brushes to the cotyledons (about 50 µL per plant). All treatments were repeated every day at the same time starting from 6th day of cultivation for eight subsequent days. Before each treatment, the cotyledons from darkness and those after MEF application were collected (day 1, 2, 4, 6 and 8). In addition, to monitor the ongoing process of aging, the amount of quantum efficiency of PSII in the cotyledons was simultaneously investigated. The collected material was placed immediately in frozen liquid nitrogen and stored at −80 °C. Each experiment was repeated at least three times (biological repetitions). All data are presented as mean ± standard error (SE).

Determination of chlorophyll content and fluorescence measurement

The chlorophyll content was determined according to Glazińska et al. (2014) with the method using Opti-Sciences CCM-200 (Opti-Sciences, Inc., USA).

Chlorophyll fluorescence changes are a useful index to measure photosynthetic efficiency. The fluorescence parameters were F 0 and F m—the initial or variable fluorescence yield, respectively, while the F v/F m ratio indicated a maximum potential of the plant’s photosynthetic ability. In our investigation, we used chlorophyll fluorometer OS-30P (Opti-Sciences, Inc., USA) according to Weng (2006). The data obtained were subjected to a statistical analysis and presented as mean ± SE. Student’s t test was used to calculate the significant differences as compared with the control.

Chloroplast detection using confocal microscopy

The collected tissue fragments were analyzed with using Nikon Eclipse TE300 confocal laser scanning inverted microscope. Obtained results were registered with an argon ion laser and He–neon laser emitting light with a wavelength of 488 nm (blue excitation and green fluorescence) and 543 nm (green excitation and red fluorescence), respectively. A mid pinhole, a long exposure time (90 s) and 100× (numerical aperture, 1.4) Plan Apochromat DCI H oil immersion lens were used in the analyses. We collected pairs of images in the green and red channels simultaneously. The EZ 2000 Viewer software package (Nikon Europe BV, Badhoevedorp, the Netherlands) was used for results documentation and analysis.

Determination of endogenous JAs

Endogenous jasmonates (jasmonic acid—JA, methyl jasmonate—JAMe) were determined with the method described by Wilmowicz et al. (2014) with modifications. Internal standards were 100 ng d2-JAMe and 100 ng d5-JA added to the crude extract. GC/MS-selected ion monitoring was performed by monitoring m/z 193, 195, 198, 224, 226 and 229.

Molecular cloning of InJMT cDNA

A full sequence of InJMT was obtained in PCR reactions using specific primers 5′-TTCCCCGAAAGCATGGGCA-3′ (forward); 5′-TCGGTTGTAGGATCGCCGGA-3′ (reverse) designed for EST fragments of InJMT (identified EST sequences of P. nil in the NCBI database: no. BJ560292.1 and CJ756546.1) (Supplementary Fig. 2). The reaction mixture was subjected to the following PCR conditions: 95 °C for 5 min, 1 cycle; 95 °C for 1 min, 62–50 °C for 30 s, 72 °C for 45 s for 15 cycles, followed by 1 cycle of incubation at 72 °C for 7 min. An amplified cDNA fragment was isolated from an agarose gel with the GeneMATRIX Agarose-Out DNA Purification Kit (EurX, Gdańsk, Poland) and sequenced by “Genomed S.A.” (Warsaw, Poland). The full-length InJMT sequence has been deposited in the GenBank database.

The bioinformatical analysis and phylogenetic tree calculation of identified cDNA was made with using ClustalW (http://www.ebi.ac.uk/clustalw), BLAST 2.2.25 (http://www.ncbi.nlm.nih.gov/BLAST) and ExPASy (http://www.expasy.org) and the CLC Main Workbench v.5.6.1 program.

Quantitative real-time PCR (qPCR)

The gene expression analysis was performed by Real-Time PCR (RT-qPCR) with a LightCycler 2.0 Carousel-Based System and LightCycler TaqMan Master Kit (ROCHE Diagnostics GmbH, Germany). The cDNA templates were prepared according to Frankowski et al. (2015), whereas gene-specific primers and UPL probes for the InJMT (GenBank acc. no. KF573520.1) were designed using Universal ProbeLibrary Assay Design Center (http://www.roche-applied-science.com/sis/rtpcr/upl) (Supplementary Table 1). InACT4 (GenBank acc. no. HM802138.2) was used as a reference gene. qPCR reaction was performed according to Frankowski et al. (2015). Three independent replicate RNA preparations were used for the analyses, and data are shown as mean ± SE of three samples for each stage.

Results

Darkness and JAMe induce cotyledon senescence in I. nil

The progress of senescence was monitored by observing the inhibition of the functional activity of PSII (F v/F m), loss of chlorophyll and reduction of its autofluorescence. Our results showed that darkness promotes senescence through chlorophyll degradation (Fig. 1b) and autofluorescence (Fig. 2b), as well as a gradual decrease of the efficiency of PSII (Fig. 1a). The effect observed is accompanied by the progressive senescence processes. The F v/F m value in dark-grown plants and in light-grown plants treated with JAMe is 15 and 20 % lower than in control plants, respectively (Fig. 1a). The same solution applied to the cotyledons of plants grown under light conditions decreased the autofluorescence of the chlorophyll (Fig. 2c). JA has no influence on senescence in light-grown plants (Fig. 1a, b). Nevertheless, the application of MEF (a jasmonate biosynthesis inhibitor) on the cotyledons of I. nil reverses the stimulatory effect of the darkness on the process discussed. These plants contain a higher chlorophyll concentration than the cotyledons of plants grown in darkness and have a higher F v/F m ratio value (Fig. 1a, b), and also, the chlorophyll contained in them demonstrates autofluorescence similar to that of the control light-grown plants (Fig. 2d).

Fig. 1
figure1

Changes in the maximum quantum efficiency of PSII [variable fluorescence (F v)/maximum fluorescence (F m) ratio] (a) and chlorophyll content (b) in the cotyledons of Ipomoea nil. CL continuous light, D darkness, JA jasmonic acid, JAMe jasmonic acid methyl ester, MEF mefenamic acid (a jasmonate biosynthesis inhibitor). Vertical bars indicate SE. Significant differences to the plants growing under light condition are indicated as **P < 0.01 and *P < 0.05

Fig. 2
figure2

Visualization of chlorophyll autofluorescence in the cotyledons of Ipomoea nil growing under: continuous light (a), dark conditions (b), treated with jasmonic acid methyl ester after transfer to continuous light (c), treated with mefenamic acid (inhibitor of jasmonate biosynthesis) after transfer to darkness (d). Bars 200 µm

Isolation of the InJMT cDNA

The full-length InJMT cDNA (GB acc. no KF573520.1) obtained using the RT-PCR technique is composed of 1403 bp and encodes 380 amino acids (Fig. 3a, b). The predicted protein sequence of InJMT contains all the conserved domains characteristic of JMT identified in other plant species. A phylogenetic analysis of the amino acid sequence revealed that the predicted InJMT is very closely related to the JMT from Solanum tuberosum (StJMT, GB acc. no XP006341965.1) (Fig. 3c).

Fig. 3
figure3

Identification of full-length InJMT cDNA. Products of PCR with specific primers (a). The coding sequence of InJMT and the deduced amino acid sequence (b). Subsequent nucleotide positions are marked on the left side of the figure, and amino acid positions on the right. The translation initiation point (the start codon) and termination point (the stop codon) are in boldface type. Small letters are used for regions not subject to translation. Domains responsible for methyl, salicylate, SAM (S-adenosyl methionine) and SAH (S-adenosyl-l-homocysteine) group binding are marked with light gray, dark gray, or are underlined, respectively. A phylogenetic analysis of InJMT with selected plant JMT sequences (c). Accession numbers of the sequences used to build the tree were as follows: RcJMT (XP002510424.1), SlJMT (XP004238275.1), StJMT (XP006341965.1), InJMT (AHA06001.1), VvJMT (XP002281588.1), TcJMT (XP007017600.1), PtJMT (XP002307671.1)

Senescence of I. nil cotyledons is accompanied by increase in the JAMe content and InJMT transcriptional activity

To investigate the possible role of JAs in cotyledon senescence in I. nil, we analyzed variations in the endogenous JA and JAMe level and the expression of InJMT coding for carboxyl methyltransferase during various stages of aging. The endogenous content of JAMe in the cotyledons of I. nil growing under darkness is initially hardly any lower than that of JA (Fig. 4b). However, the subsequent days see a gradual growth in the JAMe content in the cotyledons and a drop in the JA level. On day 8, the JAMe content is over four times that of JA, and over twice that of JAMe content in the control plants (Fig. 4b). The increased endogenous JAMe level is correlated with a stimulation of the InJMT transcriptional activity (Fig. 4a). Moreover, plants growing in darkness and treated with the jasmonate biosynthesis inhibitor (MEF) showed decrease in the InJMT expression and JAMe content (Fig. 4a, b).

Fig. 4
figure4

The expression level of InJMT (related to InACT4) in the cotyledons of I. nil seedlings during darkness conditions and following MEF treatment (a). Dark-gray bars D conditions, gray bars MEF applied during darkness conditions. The endogenous jasmonates (JA jasmonic acid, JAMe jasmonic acid methyl ester) level in the cotyledons growing under darkness conditions and JAMe after MEF treatment (b). Mean ± SE n = 3. The thick horizontal line under the time axis indicates duration of the dark period. For JA, significant differences to the plants growing under SD condition are indicated as bb P < 0.01 and b P < 0.05. For JAMe, significant differences to the plants growing under SD condition are indicated as **P < 0.01 and *P < 0.05. For JAMe, after MEF treatment, significant differences to the plants growing under darkness condition are indicated as aa P < 0.01 and a P < 0.05

Discussion

Cotyledons are organs forming during embryogenesis as initially heterotrophic, after which they become photosynthetically active, and then, their function is to provide nutrients for seedlings. The senescence of cotyledons, like in the case of other organs, is an integral part of their development; similar to leaf senescence, it is genetically programmed and occurs when they already fulfilled their role. However, this process can be stimulated prematurely by stress factors, both biotic, e.g., pathogen infection, and abiotic, e.g., extreme temperatures, drought, nutrient deficiency, and shading (Krul 1974; Quirino et al. 2000). An important role in regulating this process is played by phytohormones, which are signal molecules and/or coordinators in the transformations accompanying senescence, such as nutrient recycling, the dismantling of chloroplasts, the cell death as a consequence of DNA, RNA and proteins degradation (Krul 1974; Peterman and Siedow 1985; Quirino et al. 2000; Wada et al. 2009). Among these phytohormones, jasmonates are particularly noteworthy. These substances cause chlorophyll loss, degradation of Rubisco and inhibition of its biosynthesis. They can also induce the disorganization of the thylakoid membrane system in chloroplasts, affect the functional activity of PSII, decrease the rates of transpiration and photosynthesis, as well as cell up-regulates several SAGs shown in different plants (Ananieva et al. 2004b; Beltrano et al. 1998; He and Gan 2001; He et al. 2002; Liu et al. 2015; Weaver et al. 1998; Weidhase et al. 1987a, b; Woo et al. 2001; Oh et al. 1996). Jasmonates have been widely shown to be powerful promoters of leaf senescence (Chen and Kao 1998; Chou and Kao 1992; Hung and Kao 1996; Tsai et al. 1996; Ueda and Kato 1981; Weidhase et al. 1987a), but their participation in cotyledon senescence is still poorly understood (Ananieva et al. 2004a, 2007). In the present study, we evaluate the effects of darkness and JAs on the senescence of I. nil cotyledons.

We showed that darkness causes progressive reduction in the content of chlorophyll in the cotyledons (Fig. 1b) and decreases its autofluorescence (Fig. 2c), diminishing the F v/F m ration, as well (Fig. 1a). In many plant species, the stimulating effect of darkness on senescence processes was observed (Biswal et al. 1983; Biswal and Biswal 1984; Oh et al. 1996; Weaver et al. 1998). Reducing the endogenous jasmonate content by applying the jasmonate biosynthesis inhibitor reverses dark-stimulated cotyledon senescence in I. nil (Figs. 1a, b, 2d), which confirms that these phytohormones play a key role in regulating this process. Although the conjugate of JA and the amino acid isoleucine (Ile) has been reported to be a bioactive form of jasmonates, the results of substantial physiological and molecular research do not eliminate a specific role played by other jasmonates, such as JAMe, in controlling of growth and developmental processes (Noir et al. 2013; Pak et al. 2009; Staswick 1992). Moreover, JAMe is a plant volatile regulator and is thought to be a potential candidate for intra- and intercellular signal transducers mediating jasmonate-dependent plant responses because of its ability to diffuse through the membranes (Seo et al. 2001; Cheong and Choi 2003).

JAMe applied to the cotyledons of light-grown I. nil accelerates their senescence. Both the chlorophyll content (Fig. 1b) and the F v/F m ratio (Fig. 1a) assume values lower than in plants with dark-stimulated senescence. No such effect was observed following JA applications (Fig. 1a, b). JAs, i.e., both JA and JAMe, are promoters of leaf senescence upon their exogenous application (Creelman and Mullet 1997; Wasternack and Hause 2002). JAMe is the most effective stimulator of leaf senescence in the leaves of wild-type and mutant Arabidopsis (Oh et al. 1996; Weaver et al. 1998; Woo et al. 2001), leaf segments of barley (Reinbothe et al. 1993, 1997; Weidhase et al. 1987a) and intact cotyledons of Cucurbita pepo (zucchini) (Ananieva et al. 2004b).

Darkness leads to increased concentrations of endogenous JAMe and decreases the pool of free JA (Fig. 4b), which shows that JAMe is a cotyledon senescence stimulator in I. nil. If a change in the JAMe level is important for explaining cotyledon senescence, it is crucial to study the expression level of the InJMT gene that encodes a cytosolic protein involved in the methylation of JA. In the present investigation, we identified this gene in I. nil (Fig. 3). Based on a comparison between the deduced amino acid sequences of InJMT and JMTs from other plant species, it was found that InJMT contains all of the characteristic motifs present in SAM and SAM-like methyltransferases, SAM/SAH binding residues, salicylate/JA binding residues and additional active site residues (Fig. 3b, c), which are essential for its activity (Martin and McMillan 2002). Bearing this in mind, it can be suspected that the gene encodes a functional enzymatic protein. A progressing senescence of I. nil cotyledons is correlated with a growth in InJMT expression (Fig. 4a), which elevates the JAMe content (Fig. 4b). The drop in the endogenous JAMe content on day 8 of darkness may be caused by the exhaustion of the available substrate for the enzymatic activity of InJMT.

These results suggest that the accelerated cotyledon senescence in dark-grown I. nil is correlated with an increased InJMT expression level and, consequently, a growth in the concentration of JAMe which plays the role of a signal molecule in regulating this process.

Author contribution statement

Emilia Wilmowicz and Agata Kućko designed and carried out all the experiments and wrote the manuscript. Kamil Frankowski helped with gene identification and qPCR reactions, Michał Świdziński conducted the microscopy experiments, Katarzyna Marciniak measured the chlorophyll content and quantum efficiency of PS II, Jan Kopcewicz and Agata Kućko were responsible for preparing the final version of manuscript. All authors were responsible for analysis and interpretation of obtained results.

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Acknowledgments

This project was supported by Nicolaus Copernicus University Grant No. 1233-B and funds provided by Nicolaus Copernicus University (Toruń, Poland) for the research program of the Chair of Plant Physiology and Biotechnology.

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Communicated by P. K. Nagar.

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Wilmowicz, E., Kućko, A., Frankowski, K. et al. Methyl jasmonate-dependent senescence of cotyledons in Ipomoea nil . Acta Physiol Plant 38, 222 (2016). https://doi.org/10.1007/s11738-016-2244-1

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Keywords

  • Ipomoea nil
  • Jasmonates
  • Jasmonic acid methyl ester
  • Senescence
  • Jasmonic acid carboxyl methyltransferase