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Effects of endogenous salicylic acid synthesized through PAL and ICS pathway on baicalin and baicalein accumulation in Scutellaria baicalensis Georgi

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Abstract

The aim of this study was to investigate the effect of phenylalanine ammonia lyase (PAL) and isochorismate synthase (ICS) on free salicylic acid (FSA) or total salicylic acid (TSA) content, and the effect of endogenous SA on baicalin and baicalein accumulation in Scutellaria baicalensis Georgi, respectively. We amplified partial sequences of PAL and ICS genes in Scutellaria baicalensis Georgi and silenced the two genes with virus-induced gene silence (VIGS) technique, respectively. The influence of gene silence on FSA, TSA, baicalin, and baicalein accumulation in Scutellaria baicalensis Georgi were analyzed, and these parameters were also investigated under high temperature. Results indicated that PAL silence significantly affected the FSA, ICS affected TSA content. FSA significantly affected the baicalin, rather than baicalein content. Our results along with previous studies indicated PAL and ICS were different in the regulation of FSA or TSA synthesis, and FSA and TSA were different in the regulation of baicalin and baicalein synthesis in Scutellaria baicalensis Georgi.

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Abbreviations

SA:

Salicylic acid

PAL:

Phenylalanine ammonia lyase

ICS:

Isochorismate synthase

FSA:

Free salicylic acid

TSA:

Total salicylic acid

VIGS:

Virus induced gene silence

IPL:

Isochorismate pyruvate-lyase

HT:

High temperature

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81102803) and “555 talent project” of Jiangxi Province, Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In Vitro Diagnostic Reagents and Devices of Jiangxi Province (Grant no. 31171731, 31460447, 20142BDH80003, 2013-CXTD002).

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Authors and Affiliations

  1. Life Science Department, Nanchang University, Nanchang, Jiangxi province, China

    Hu Su, Chao Yu, Junjun Shang, Xin Yan, Pengfei Liao & Youlin Zhu

  2. Life Science Department, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi province, China

    Hu Su, Yanchun Gong & Bin Zeng

Authors
  1. Hu Su
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  2. Chao Yu
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  3. Junjun Shang
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  4. Xin Yan
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  5. Pengfei Liao
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  6. Youlin Zhu
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  7. Yanchun Gong
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  8. Bin Zeng
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Corresponding author

Correspondence to Youlin Zhu.

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Communicated by A. Krolicka.

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Su, H., Yu, C., Shang, J. et al. Effects of endogenous salicylic acid synthesized through PAL and ICS pathway on baicalin and baicalein accumulation in Scutellaria baicalensis Georgi. Acta Physiol Plant 38, 178 (2016). https://doi.org/10.1007/s11738-016-2193-8

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  • DOI: https://doi.org/10.1007/s11738-016-2193-8

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