Abstract
Phosphoethanolamine N-methyltransferase (encoded by the PEAMT gene) catalyzes the methylation of phosphoethanolamine to produce phosphocholine, which is dephosphorylated to free choline. Choline is the substrate for glycine betaine synthesis, which can improve the salt tolerance of plants. A 1688 bp cDNA of SlPEAMT was isolated from the halophyte Suaeda liaotungensis. Real-time quantitative PCR showed that SlPEAMT expression was induced in S. liaotungensis under salt stress. An 897 bp fragment of the 5′ flanking region of SlPEAMT (designated pP) was isolated from S. liaotungensis. This fragment contained the transcription start site and a number of stress-responsive elements, such as MBS, GT-1 elements, and a W-box. The fragment was inserted into the pCAMBIA1301 plasmid by replacing the CaMV35S promoter. The pP-GUS construct was introduced into tobacco. β-glucuronidase (GUS) histochemical staining and quantitative fluorescence analysis showed an 18.6-fold increase in GUS activity in pP-GUS transgenic tobacco treated with 200 mmol NaCl l−1. Thus, SlPEAMT and its promoter are responsive to salt stress and may be useful in genetic engineering to improve plant salt tolerance.
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References
Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78
Bolognese CP, McGraw P (2000) The isolation and characterization in yeast of a gene for Arabidopsis S-adenosylmethionine: phospho-ethanolamine N-methyltransferase. Plant Physiol 124:1800–1813
Chen C, Chen Z (2002) Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol 129:706–716
Chinnusamy V, Ohta M, Kanrar S, Lee BH, Hong X, Agarwal M, Zhu JK (2003) ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev 17:1043–1054
Datko AH, Mudd SH (1988) Enzymes of phosphatidylcholine synthesis in Lemna, soybean and carrot. Plant Physiol 88:1338–1348
Dunn MA, White AJ, Vural S, Hughes MA (1998) Identification of promoter elements in a low-temperature-responsive gene (blt4.9) from barley (Hordeum vulgare L.). Plant Mol Biol 38:551–564
Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database. Nucleic Acids Res 21:297–300
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
McNeil SD, Rhodes D, Russell BL, Nuccio ML, Shachar-Hill Y, Hanson AD (2000) Metabolic modeling identifies key constraints on an engineered glycine betaine synthesis pathway in tobacco. Plant Physiol 124:153–162
Nuccio ML, McNeil SD, Ziemak MJ, Hanson AD (2000) Choline import into chloroplasts limits Glycine betaine synthesis in tobacco: analysis of plants engineered with a chloroplastic or a cytosolic pathway. Metab Eng 2:300–311
Park HC, Kim ML, KangH Y, JeonM J, Yoo JH, Kim MC, Park CY, Jeong JC, Moon BC, Lee JH, Yoon HW, Lee SH, ChungS W, Lim CO, Lee SY, Hong JC, Cho MJ (2004) Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor. Plant Physiol 135:2150–2161
Rhodes D, Hanson AD (1993) Quaternary ammonium and tertiary sulfonium compounds in higher plants. Annu Rev Plant Physiol Plant Mol Biol 44:357–384
Shahmuradov IA, Solovyev VV, Gammerman AJ (2005) Plant promoter Prediction with confidence estimation. Nucleic Acids Res 333:1069–1076
Tabuchi T, Okada T, Takashima Y, Azuma T, Nanmori T, Yasuda T (2006) Transcriptional response of glycinebetaine-related genes to salt stress and light in leaf beet. Plant Biotechnol 23:317–320
Urao T, Yamaguchi-Shinozaki K, UraoS Shinozaki K (1993) An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell 5:1529–1539
Wu S, Yu Z, Wang F, Li W, Ye C, Li J, Tang J, Ding J, Zhao J, Wang B (2007) Cloning, characterization, and transformation of the phosphoethanolamine N-methyltransferase gene (ZmPEAMT1) in maize (Zea mays L.). Mol Biotechnol 36:102–112
Yancey PH (1994) Compatible and counteracting solutes. In: Strange K (ed) Cellular and molecular physiology of cell, vol regulation. CRC Press Inc, Boca Raton, pp 81–109
Ye C, Wu S, Yang Q, Ma C, Yang G, Wang B (2005) Cloning, sequencing and salt induced expression of PEAMT and BADH in oilseed rape (Brassica napus). DNA Seq 16:364–371
Zhang Y, Yin H, Li D, Zhu W, Li Q (2008) Functional analysis of BADH gene promoter from Suaeda liaotungensis K. Plant Cell Rep 27:585–592
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This work was supported by the Science and Technology Project of Dalian, China (Grant No. 2007J23JH025).
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Li, QL., Xie, JH., Ma, XQ. et al. Molecular cloning of Phosphoethanolamine N-methyltransferase (PEAMT) gene and its promoter from the halophyte Suaeda liaotungensis and their response to salt stress. Acta Physiol Plant 38, 39 (2016). https://doi.org/10.1007/s11738-016-2063-4
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DOI: https://doi.org/10.1007/s11738-016-2063-4