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In vitro response of black gram genotypes to herbicide stress and elevation of antioxidative defence system

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Abstract

In vitro response of seedlings of six genotypes of Vigna mungo L. Hepper to herbicide stress, induced by glyphosate was assessed by evaluating its effect on morphological parameters viz. germination percentage, survival percentage, shoot length, root length, fresh weight, as well as on biochemical parameters such as activity of antioxidative enzymes (catalase, peroxidase and superoxide dismutase), total protein and proline content along with MDA content. Findings indicate that herbicide treatment promotes significant and dose-dependent decrease in all morphological parameters and increase in activity of antioxidative enzymes in all the genotypes. Herbicide treatment promotes significant decrease in total protein content and increase in proline content. Significant variation for MDA content among genotypes was not observed. On the basis of results of present investigation, it was concluded that Vigna mungo genotypes tolerant to the herbicide stress can be of considerable practical value for studying the mechanism of herbicide tolerance and for providing genetic resources for the development of herbicide-tolerant cultivars.

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Acknowledgments

Authors thankfully acknowledge the Council of Scientific and Industrial Research (CSIR), New Delhi, India for financial support for the present study and Indian Institute of Pulse Research (IIPR), Kanpur, India for providing seeds of black gram genotypes.

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  1. In vitro Culture and Plant Genetics Unit, Department of Botany, Faculty of Science, University of Lucknow, Lucknow, 226007, India

    Jyoti Bajpai & Alka Srivastava

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  1. Jyoti Bajpai
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  2. Alka Srivastava
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Correspondence to Alka Srivastava.

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Communicated by M. Lambardi.

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Bajpai, J., Srivastava, A. In vitro response of black gram genotypes to herbicide stress and elevation of antioxidative defence system. Acta Physiol Plant 37, 182 (2015). https://doi.org/10.1007/s11738-015-1926-4

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  • DOI: https://doi.org/10.1007/s11738-015-1926-4

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