Abstract
Drought is one of the main limiting factors for peanut yield worldwide. However, the underlying genetic and molecular mechanisms remain unclear. In this study, we identified several water deficiency tolerant peanut germplasms based on physiological properties in the seedling stage. One of the germplasms was further characterized by transcriptome sequencing of samples that had been untreated or treated with PEG 6000 at three different times. A total of 370,145 non-redundant transcripts and 141,289 unigenes were obtained, and differentially expressed transcripts were identified among samples. Based on functional annotation, transcripts involved in drought response pathways were selected for qPCR analysis. Similar patterns but different levels of gene expression were found between drought-tolerant and drought-sensitive germplasms. These findings provide useful insights into drought tolerance of peanut.
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Acknowledgments
This work was funded by SCX(12)5039 (The Self-directed Innovation Fund of Agricultural Science and Technology in Jiangsu Province, China). Zhiguo E is responsible for qPCR, and Xiaojun Zhang helped with data arrangement.
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The authors declare that they have no conflict of interest.
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Communicated by M. Hajduch.
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Supplementary material 1 (TIFF 51 kb) Supplemental Fig. 1: Relative seedling heights after 7 days treatment with water (CK) and PEG 6000 of 50 peanut germplasms
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Supplementary material 2 (XLSX 10 kb) Supplemental Table 1: Names and origins of 50 peanut germplasms for tolerance screening
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Supplementary material 3 (XLSX 14930 kb) Supplemental Table 2: Detailed unigene information, including length and various annotations compared in different databases
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Supplementary material 4 (XLSX 1368 kb) Supplemental Table 3: KEGG pathway anchoring of unigenes, including pathway name, pathway type, related gene number, and detailed gene ID list
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Supplementary material 5 (XLSX 33 kb) Supplemental Table 4: Gradually increased (Sheet 1) or decreased (Sheet 2) transcripts after drought treatment
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Shen, Y., Zhiguo, E., Zhang, X. et al. Screening and transcriptome analysis of water deficiency tolerant germplasms in peanut (Arachis hypogaea). Acta Physiol Plant 37, 103 (2015). https://doi.org/10.1007/s11738-015-1840-9
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DOI: https://doi.org/10.1007/s11738-015-1840-9