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Isolation and expression of CsFAD7 and CsFAD8, two genes encoding ω-3 fatty acid desaturase from Camellia sinensis

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Abstract

The plastidial ω-3 fatty acid desaturase catalyses the production of trienoic fatty acids (TAs) in plant chloroplasts and plays an important role in plant responses to environmental stress. In this study, the full-length cDNAs encoding two plastidial ω-3 desaturases, designated CsFAD7 and CsFAD8 (GenBank Accession No. JX943516 and KC847167, respectively), were isolated from the tea plant (Camellia sinensis L.) using RT-PCR and RACE. Codon usage analysis revealed that U- and A-ended codons were preferentially used in these two genes. Sequence analysis showed that the deduced amino acid sequences of CsFAD7 and CsFAD8 had high homology to plastidial ω-3 desaturases from other plant species. Expression analysis by real-time PCR revealed that both genes are tissue-specific and expressed the highest levels in shoots. Meanwhile, CsFAD7 and CsFAD8 responded to various abiotic stresses and hormones, but in very different manners. Taken together, these results suggest that CsFAD7 and CsFAD8 are both responsive to abiotic stress signals; however, they may play very different roles during stress tolerance in tea plants.

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Acknowledgments

This research was funded by a Sci-Tech development project of Shandong province (2011GNC11010) and the National Science and Technology Support Program (2011BAD01B01).

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Authors and Affiliations

  1. Tea Research Institute, Qingdao Agricultural University, Qingdao, 266109, Shandong, China

    Qing-Ping Ma, En You, Jing Wang, Yu Wang & Zhao-Tang Ding

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  1. Qing-Ping Ma
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  2. En You
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  3. Jing Wang
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  4. Yu Wang
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  5. Zhao-Tang Ding
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Correspondence to Zhao-Tang Ding.

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Communicated by E. Schleiff.

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Ma, QP., You, E., Wang, J. et al. Isolation and expression of CsFAD7 and CsFAD8, two genes encoding ω-3 fatty acid desaturase from Camellia sinensis . Acta Physiol Plant 36, 2345–2352 (2014). https://doi.org/10.1007/s11738-014-1607-8

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  • DOI: https://doi.org/10.1007/s11738-014-1607-8

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