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Overexpression of tomato chloroplast omega-3 fatty acid desaturase gene alleviates the photoinhibition of photosystems 2 and 1 under chilling stress

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Photosynthetica

Abstract

In transgenic (TG) tomato (Lycopersicon esculentum Mill.) overexpressed ω-3 fatty acid desaturase gene (LeFAD7) was identified, which was controlled by the cauliflower mosaic virus 35S promoter and induced increased contents of unsaturated fatty acids in thylakoid membrane. Under chilling stress at low irradiance (4 °C, 100 µmol m−2 s−1) TG plants with higher linolenic acids (18: 3) content maintained a higher O2 evolution rate, oxidizable P700 content, and maximal photochemical efficiency (Fv/Fm) than wild type (WT) plants. Low temperature treatment for 6 h resulted in extensive changes of chloroplast ultrastructure: in WT plants most chloroplasts became circular, the number of amyloids increased, appressed granum stacks were dissolved, grana disappeared, and the number of grana decreased, while only a few grana were found in leaves of TG plants. Hence the overexpression of LeFAD7 could increase the content of 18: 3 in thylakoid membrane, and this increase alleviated the photoinhibition of photosystem (PS) 1 and PS2 under chilling at low irradiance.

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Abbreviations

DAs:

dienoic fatty acids

DGDG:

digalactosyldiacylglycerol

Fv/Fm :

maximal photochemical efficiency of photosystem 2

JA:

jasmonic acid

LeFAD7:

Lycopersicon esculentum ω-3 fatty acid desaturase gene

MGDG:

monogalactosyldiacylglycerol

P700:

photosystem 1 reaction centre

PC:

phosphatidylcholine

PFD:

photon flux density

PG:

phosphatidylglycerol

PI:

phosphatidylinositol

SQDG:

sulfoquinovosyldiacylglycerol

TAs:

trienoic fatty acids

TG:

transgenic

16: 1(3t):

trans-hexadecenoic acids

16: 3:

hexadecatrienoic acids

18: 1:

oleic acid

18: 2:

linoleic acid

18: 3:

linolenic acids

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Authors and Affiliations

  1. School of Medicine, Zhejiang University, Hangzhou, 310058, P.R. China

    X.-Y. Liu

  2. College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, P.R. China

    X.-Y. Liu, B. Li, N. Sui, X.-M. Yang & Q.-W. Meng

  3. Department of Horticulture, Zhejiang University, Hangzhou, 310029, P.R. China

    J.-H. Yang

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  1. X.-Y. Liu
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  2. B. Li
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  3. J.-H. Yang
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  4. N. Sui
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  6. Q.-W. Meng
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Correspondence to Q.-W. Meng.

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Liu, XY., Li, B., Yang, JH. et al. Overexpression of tomato chloroplast omega-3 fatty acid desaturase gene alleviates the photoinhibition of photosystems 2 and 1 under chilling stress. Photosynthetica 46, 185–192 (2008). https://doi.org/10.1007/s11099-008-0030-z

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  • DOI: https://doi.org/10.1007/s11099-008-0030-z

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