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Sulfur stress-induced antioxidative responses in leaves of Triticum aestivum L.

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Abstract

Antioxidative responses were investigated in leaves of wheat (Triticum aestivum L.) grown at varying S levels ranging from deficiency to excess (1, 2, 4, 6 and 8 mM S). Optimum yield was observed in plants supplied with 4 mM S. Wheat responded to S deficiency and excess supply by decreasing growth of root and shoot. Chlorosis in young leaves was observed after 15 days of deficient S supply. The biomass and concentration of photoassimilatory pigments decreased in plants grown at 1, 2, 6 and 8 mM S supply. The concentration of thiobarbituric acid reactive substances (TBARS), cysteine, nonprotein thiol and hydrogen peroxide (H2O2) increased in plants grown under S stress. Accumulation of TBARS and H2O2 in leaves indicated oxidative damage in S-deficient and S-excess plants. Deficient and excess levels of S showed an increase in the activities of antioxidative enzymes superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6), peroxidase (EC 1.11.1.7), ascorbate peroxidase (EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2).

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Abbreviations

APX:

Ascorbate peroxidase

AsA:

Ascorbate

CAT:

Catalase

Cys:

Cysteine

GR:

Glutathione reductase

H2O2 :

Hydrogen peroxide

NPT:

Nonprotein thiol

\(^{ \cdot } {\text{OH}}\) :

Hydroxyl radical

1O2 :

Singlet oxygen

\({\text{O}}_{ 2}^{ \cdot }\) :

Superoxide radical

POD:

Peroxidase

ROS:

Reactive oxygen species

S:

Sulfur

SOD:

Superoxide dismutase

TBARS:

Thiobarbituric acid reactive substances

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Authors and Affiliations

  1. Plant Nutrition and Stress Physiology Laboratory, Department of Botany, University of Lucknow, Lucknow, 226007, India

    Neelam Chandra & Nalini Pandey

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  1. Neelam Chandra
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  2. Nalini Pandey
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Correspondence to Nalini Pandey.

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Communicated by G. Bartosz.

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Chandra, N., Pandey, N. Sulfur stress-induced antioxidative responses in leaves of Triticum aestivum L.. Acta Physiol Plant 36, 2079–2089 (2014). https://doi.org/10.1007/s11738-014-1585-x

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  • DOI: https://doi.org/10.1007/s11738-014-1585-x

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