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Effect of water restriction on total phenolics and antioxidant properties of Amburana cearensis (Fr. Allem) A.C. Smith cotyledons during seed imbibition

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Abstract

Amburana cearensis (Fr. Allem) AC Smith is a native Caatinga species commonly found in the semiarid region of North East of Brazil, which possesses resistance to temperature and drought stress and is widely used in the folk medicine. This study aimed at a better understanding of seed imbibition and germination as well as to evaluate the effect of water restriction on total phenolics and antioxidant properties in cotyledons of A. cearensis (Fr. Allem) A.C. Smith. Seeds were imbibed in water (control) or PEG 8000 solution (−1.2 and −1.4 MPa). Germinability was evaluated up to 180 h of seed imbibition, whereas cotyledons were collected at 0, 24, 48, 72 and 96 h of imbibition. The radical scavenging ability was enhanced in response to the water restriction after 96 h of imbibition as compared to the control. Water restriction affected water uptake and seed germinability while inducing a faster response in terms of biosynthesis of phenolic compounds, although total phenolic content decreased as compared to control. There is a high correlation between total phenolics and antioxidant activity for the seeds subjected to the water restriction conditions. These findings provide insights into the mechanisms underlying plant adaptation to harsh environmental conditions during germination, which would be very helpful to develop germination and seedling production protocols aimed at strategies for the sustainable exploitation of A. cearensis by local folk communities under the typical harsh conditions of the Brazilian Caatinga and semiarid regions.

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References

  • Andarwulan N, Fardiaz D, Wattimena GA, Shetty K (1999) Antioxidant activity associated with lipid and phenolic mobilization during seed germination of Pangium edule Reinw. J Agric Food Chem 47(8):3158–3163

    Article  CAS  PubMed  Google Scholar 

  • Antunes CGC, Pelacani CR, Ribeiro RC, de Souza JV, de Souza CLM, de Castro RD (2011) Germination of Caesalpinia pyramidalis Tul. seeds (Catingueira) under water deficit (Germinação De Sementes De Caesalpinia pyramidalis Tul. (Catingueira) Submetidas A Deficiência Hídrica). Rev Arvore 35(5):1007–1015

    Article  Google Scholar 

  • Bewley JD, Bradford KJ, Hilhorst HWM, Nonogaki H (2013) Seeds—physiology of development, germination and dormancy. Springer, New York

    Google Scholar 

  • Canuto KM, Silveira ER (2006) Chemical constituents of trunk bark of Amburana cearensis A.C. Smith (Constituintes químicos da casca do caule de Amburana cearensis A.C. Smith). Quim Nova 29(6):1241–1243

    Article  CAS  Google Scholar 

  • Canuto KM, Lima MAS, Silveira ER (2010) Amburosides c-h and 6-o-protocatechuoyl coumarin from Amburana cearensis. J Braz Chem Soc 21(9):1746–1753

    Article  CAS  Google Scholar 

  • Cevallos-Casals BA, Cisneros-Zevallos L (2010) Impact of germination on phenolic content and antioxidant activity of 13 edible seed species. Food Chem 119(4):1485–1490

    Article  CAS  Google Scholar 

  • Costa-Lotufo LV, Jimenez PC, Wilke DV, Leal LKAM, Cunha GMA, Silveira ER, Canuto KM, Viana GSB, Moraes MEA, De Moraes MO, Pessoa C (2003) Antiproliferative effects of several compounds isolated from Amburana cearensis A. C. Smith. Z Naturforsch Sect C. J Biosci 58(9–10):675–680

    CAS  Google Scholar 

  • Guo X, Li T, Tang K, Liu RH (2012) Effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts (Vigna radiata). J Agric Food Chem 60(44):11050–11055

    Article  CAS  PubMed  Google Scholar 

  • Kim HY, Lee SH, Hwang IG, Woo KS, Kim KJ, Lee MJ, Kim DJ, Kim TJ, Lee J, Jeong HS (2013) Antioxidant and antiproliferation activities of winter cereal crops before and after germination. Food Sci Biotechnol 22(1):181–186

    Article  CAS  Google Scholar 

  • Leal LK, Fonseca FN, Pereira FA, Canuto KM, Felipe CFB, Fontenele JB, Pitombeira MV, Silveira ER, Viana GSB (2008) Protective effects of amburoside A, a phenol glucoside from Amburana cearensis, against CCl4-induced hepatotoxicity in rats. Planta Med 74(5):497–502

    Article  CAS  PubMed  Google Scholar 

  • Leal LK, Canuto KM, Da Silva Costa KC, Nobre-Júnior HV, Vasconcelos SM, Silveira ER, Ferreira MV, Fontenele JB, Andrade GM, De Barros Viana GS (2009) Effects of amburoside a and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils. Basic Clin Pharmacol Toxicol 104(3):198–205

    Article  CAS  PubMed  Google Scholar 

  • Lee YR, Woo KS, Kim KJ, Son JR, Jeong HS (2007) Antioxidant activities of ethanol extracts from germinated specialty rough rice. Food Sci Biotechnol 16(5):765–770

    CAS  Google Scholar 

  • Leite EJ (2005) State-of-knowledge on Amburana cearensis (Fr. Allem.) A.C. Smith (Leguminosae: Papilionoideae) for genetic conservation in Brazil. J Nat Conserv 13(1):49–65

    Article  Google Scholar 

  • López-Amorós ML, Hernández T, Estrella I (2006) Effect of germination on legume phenolic compounds and their antioxidant activity. J Food Compos Anal 19(4):277–283

    Article  Google Scholar 

  • Lorenzi H, Matos FJA (2002) Plantas medicinais no Brasil; nativas e exóticas. Instituto Plantarum, Nova Odessa

    Google Scholar 

  • Roesler R, Malta LG, Carrasco LC, Holanda RB, Sousa CAS, Pastore GM (2007) Antioxidant activity of cerrado fruits (Atividade antioxidante de frutas do cerrado). Cienc Tecnol Aliment 27(1):53–60

    Article  CAS  Google Scholar 

  • Sousa CMDM, Silva HRE, Vieira GM Jr, Ayres MCC, Da Costa CLS, Araújo DS, Cavalcante LCD, Barros EDS, Araújo PBDM, Brandão MS, Chaves MH (2007) Total phenolics and antioxidant activity of five medicinal plants (Fenóis totais e atividade antioxidante de cinco plantas medicinais). Quim Nova 30(2):351–355

    Article  CAS  Google Scholar 

  • Virgens IO, de Castro RD, Fernandez LG, Pelacani CR (2012) Physiologic behavior of Myracrodruon urundeuva (Anacardiaceae) fr. all. seeds submitted to abiotic factors (Comportamento fisiológico de sementes de Myracrodruon urundeuva fr. all. (Anacardiaceae) submetidas a fatores abióticos). Cienc Florest 22(4):681–692

    Google Scholar 

  • Weidner S, Karamać M, Amarowicz R, Szypulska E, Gołgowska A (2007) Changes in composition of phenolic compounds and antioxidant properties of Vitis amurensis seeds germinated under osmotic stress. Acta Physiol Plant 29(3):283–290

    Article  CAS  Google Scholar 

  • Weidner S, Brosowska-Arendt W, Szczechura W, Karamać M, Kosińska A, Amarowicz R (2011) Effect of osmotic stress and post-stress recovery on the content of phenolics and properties of antioxidants in germinating seeds of grapevine Vitis California. Acta Soc Bot Pol 80(1):11–19

    Article  CAS  Google Scholar 

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Acknowledgments

Financial support was given by Conselho Nacional de Desenvolvimento Científico e Tecnológico and the Northeast Biotechnology Network (CNPq/RENORBIO, Project No. 554839/2006-7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/REUNI)—Brazil.

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Authors and Affiliations

  1. Laboratório de Bioquímica, Biotecnologia e Bioprodutos, Departamento de Biofunção, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil

    Erica P. L. Pereira, Paulo R. Ribeiro, Marta B. Loureiro, Renato D. de Castro & Luzimar G. Fernandez

Authors
  1. Erica P. L. Pereira
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  2. Paulo R. Ribeiro
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  3. Marta B. Loureiro
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  4. Renato D. de Castro
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  5. Luzimar G. Fernandez
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Corresponding author

Correspondence to Luzimar G. Fernandez.

Additional information

Communicated by M. Horbowicz.

E. P. L. Pereira and P. R. Ribeiro contributed equally to this work and should both be considered first author.

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Pereira, E.P.L., Ribeiro, P.R., Loureiro, M.B. et al. Effect of water restriction on total phenolics and antioxidant properties of Amburana cearensis (Fr. Allem) A.C. Smith cotyledons during seed imbibition. Acta Physiol Plant 36, 1293–1297 (2014). https://doi.org/10.1007/s11738-014-1503-2

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  • DOI: https://doi.org/10.1007/s11738-014-1503-2

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