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Hydrogen peroxide is involved in the regulation of rice (Oryza sativa L.) tolerance to salt stress

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Abstract

In the present study, we investigated the salt tolerance mechanism of two rice cultivars (Zhenghan-2 and Yujing-6), which show different tolerance to drought and disease. NaCl induced higher extent of lipid peroxide and ion leakage in Yujing-6 roots than those in Zhenghan-2 roots. H2O2 accumulation in Zhenghan-2 roots was lower than that in Yujing-6 roots under salt stress. Comparatively, NaCl treatment did not increase O2 − contents in both rice roots, however, O2 − level in Yujing-6 roots was higher than that in Zhenghan-2 roots under both control and salt stress conditions. Ascorbate peroxidases (APX) activity increased more significantly in Zhenghan-2 roots than that in Yujing-6 roots. The activity of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and glucose-6-phosphate dehydrogenase (G6PDH) was similarly enhanced in both rice roots under salt stress; however, they showed higher levels in Zhenghan-2 roots than in Yujing-6 roots. Exogenous H2O2 could enhance APX, CAT, POD, SOD and G6PDH activities in a concentration-dependent manner in both rice roots. Diphenylene iodonium (DPI), a plasma membrane (PM) NADPH oxidase inhibitor, which counteracted the NaCl-induced H2O2 accumulation, markedly decreased the activity of above enzymes. Moreover, ion leakage increased dramatically in Zhenghan-2 roots and reached to the similar level of Yujing-6 roots under NaCl+DPI treatment. Taken together, H2O2, which is mainly generated from PM NADPH oxidase, is involved in Zhenghan-2 rice tolerance to salt stress by enhancing the cellular antioxidant level.

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Abbreviations

APX:

Ascorbate peroxidases

CAT:

Catalases

DPI:

Diphenylene iodonium

G6PDH:

Glucose-6-phosphate dehydrogenase

GR:

Glutathione reductase

H2O2 :

Hydrogen peroxide

O2 − :

Superoxide anion radical

OH· :

Hydroxyl radical

PM:

Plasma membrane

POD:

Peroxidases

ROS:

Reactive oxygen species

SOD:

Superoxide dismutases

TBARS:

TBA-reactive substances

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31170225; 31201145), the National High Technology Research and Development Program (2007AA021401), Foundation of Science and Technology Program of Gansu Province (1107RJYA005), Fundamental Research Funds for the Central Universities (lzujbky-2012-104) and Chunhuijihua Foundation (Z2008-1-62015).

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Authors and Affiliations

  1. Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Xiaomin Wang, Chen Hou, Jie Liu, Wenliang He, Wenbin Nan, Huiling Gong & Yurong Bi

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  1. Xiaomin Wang
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  2. Chen Hou
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  3. Jie Liu
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  4. Wenliang He
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  6. Huiling Gong
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  7. Yurong Bi
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Correspondence to Yurong Bi.

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Communicated by G. Bartosz.

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Wang, X., Hou, C., Liu, J. et al. Hydrogen peroxide is involved in the regulation of rice (Oryza sativa L.) tolerance to salt stress. Acta Physiol Plant 35, 891–900 (2013). https://doi.org/10.1007/s11738-012-1132-6

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  • DOI: https://doi.org/10.1007/s11738-012-1132-6

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