Skip to main content

Advertisement

SpringerLink
Log in

In vitro mass production of Ruta gravoelens L. for secondary products production

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Hypocotyls explants of 1-week-old Ruta gravoelens L. seedlings showed high regeneration ability on Murashige and Skoog’s medium supplemented with 5.2 mM H2PO4 −, 250 mM adenine sulphate, 3.0% sucrose and 8.87 μM BA. Increasing phosphate and adenine sulphate concentrations had pronounced effects on organogenesis and shoot growth compared to the stander MS medium. Multiple shoots were successfully maintained for 60 days in static liquid medium without any hyperhydricity phenomena. Uncountable shoot buds and shoots were developed on the leaf surface which was in contact with the medium. The growth index increased linearly during the culture course (R 2 = 0.992–0.999) and the highest GI (30.43) was achieved on medium enriched with 90 mM nitrogen and 4.5% sucrose. Medium supplemented with 6.0% sucrose and 60 mM N yielded the highest biomass (4.37 g per flask). However, medium with the stander N content and 6.0% sucrose developed the maximum percentage of biomass (6.67%). The highest percentage of furanocoumarins (11.46 mg g−1 dry weight) calculated as xanthotoxin was produced in medium enriched with 90 mM N and 4.5 or 6.0% sucrose, whereas the maximum rutin (73.5 mg g−1 dry weight) was on medium supplemented with 90 mM N and 4.5% sucrose. Developing system seems to be used for secondary metabolites production because the shoot rooting did not appear. So, all energy is devoted for shoots multiplication, their growth and secondary metabolites production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

BA:

6-Benzylaminopurine

DW:

Dry weight

FUC:

Furanocoumarins

FW:

Fresh weight

GI:

Growth index

MS:

Murashige and Skoog’s medium

MSM:

Modified Murashige and Skoog’s medium

References

  • Alfermann AW, Petersen M (1995) Natural product formation by plant cell biotechnology. Plant Cell Tissue Organ Cult 43:199–205

    Article  CAS  Google Scholar 

  • Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice, 2nd edn. Elsevier Science, Amsterdam

    Google Scholar 

  • Bisaccia E (1993) Extracorporeal photopheresis: complex extended trial in treatment of AIDS. J AIDS 6:386–389

    CAS  Google Scholar 

  • Bohidar S, Thirunavoukkarasu M, Rao TV (2008) Effect of plant growth regulators on in vitro micropropagation of “Garden Rue” (Ruta gravoelens L.). Int J Integr Biol 3:36–43

    CAS  Google Scholar 

  • Bruneton J (1987) Elements de Phytochimie et de Pharmacognosie. Technique et Documentation, Lavoisier 161–163

  • Carlo GD, Mascolo N, Izzo AA, Capasso F (1999) Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci 65:337–353

    Article  PubMed  Google Scholar 

  • Chandler SF, Dodds JH (1983) The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Rpt 2:105–108

    Google Scholar 

  • Clewer AG, Scarisbrick DH (2001) practical statistics and experimental design for plant and crop science. Wiley and Sons, Chicheter, New York

    Google Scholar 

  • Collin HA (2001) Secondary product formation in plant tissue cultures. Plant Growth Regul 34:119–134

    Article  CAS  Google Scholar 

  • De Castro RL, De Barros IBI (1999) Micropropagation of rue (Ruta gravoelens L.). Acta Hortic (ISHS) 502:325–328

    Google Scholar 

  • Diwan R, Malpathak N (2007) A novel source of furanocoumarins: Ruta gravoelens L. In: Kumar A, Sopory S (eds) Recent advances in plant biotechnology, vol 11. International publishers, New Delhi, pp 158–187

  • Diwan R, Malpathak N (2008) Novel technique for scaling up of micropropagated Ruta gravoelens shoots using liquid culture systems: a step towards commercialization. New Biotechnol 25:85–91

    Article  CAS  Google Scholar 

  • Egyptian pharmacopoeia (1984) vol I, 3rd edn, pp 204–205

  • Ekiert H, Chooniewska M, Gomóka E (2001) Accumulation of furanocoumarins in Ruta gravoelens L. shoot culture. Biotechnol Lett 23:543–545

    Article  CAS  Google Scholar 

  • George EF, De Klerk G-J (2008) The components of plant tissue culture media I: macro- and micro-nutrients. In: George EF, Hall MA, De Klerk G-J (eds) Plant propagation by tissue culture. Part I: The technology, 3rd edn. Springer, pp 65–114

  • Grinberg LN, Rachmilewitz EA, Newmark H (1994) Protective effects of rutin against hemoglobin oxidation. Biochem Pharmacol 48:643–649

    Article  PubMed  CAS  Google Scholar 

  • Harborne JB (1988) The flavonoids. Chapman and Hall, London, pp 622–645

    Google Scholar 

  • Leifert C, Murphy KP, Lumsden PJ (1995) Mineral and carbohydrate nutrition of plant cell and tissue cultures. Crit Rev Plant Sci 14:83–109

    CAS  Google Scholar 

  • Lievre K, Hehna A, Trana TL, Thomasset AGB, Bourgaud F, Gontiera E (2005) Genetic transformation of the medicinal plant Ruta gravoelens L. by an Agrobacterium tumefaciens-mediated method. Plant Sci 168:883–888

    Article  CAS  Google Scholar 

  • Mohamed MAH (2011) A protocol for the mass micropropagation of carnation (Dianthus caryophyllus L.).J Hortic Sci Biotechnol 86:135–140

    Google Scholar 

  • Mors WB, Nascimento MC, Pereira BMR, Pereira NA (2000) Plant natural products active against snake bite—the molecular approach. Phytochemistry 55:627–642

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for growth and bioassays with tobacco tissue cultures. Physiol Plant 15:437–497

    Article  Google Scholar 

  • Neumann K, Kumar A, Imani J (2009) Plant cell and tissue culture—a tool in biotechnology basics and application. Springer

  • Nwankwo BA, Krikorian AD (1983) Morpho-genetic potential of embryo- and seedling-derived callus of Elaeis guineensis Jacq. var. pisifera Becc. Ann Bot 51:65–76

    Google Scholar 

  • Ramage CM, Williams RR (2002) Mineral nutrition and plant morphogenesis. In Vitro Cell Dev Biol Plant 38:116–124

    Article  CAS  Google Scholar 

  • Renuka D, Nutan M (2010) Histochemical localization in Ruta gravoelens cell culture: elucidating the relationship between cellular differentiation and furanocoumarin production. In Vitro Cell Dev Biol Plant 46:108–116

    Article  Google Scholar 

  • Robak J, Gryglewski RJ (1988) Flavonoids are scavenger of superoxide anions. Biochem Pharmacol 37:837–841

    Article  PubMed  CAS  Google Scholar 

  • Sambantham P (1985) Analgesic activity of certain bioflavonoids. Indian J Pharm Sci 47:230–231

    CAS  Google Scholar 

  • Steck W, Bailey Bk, Shyluk JP, Gamborg OL (1971) Coumarins and alkaloids from cell cultures of Ruta gravoelens. Phytochemistry 10:191–194

    Article  CAS  Google Scholar 

  • Van Staden J, Zazimalova E, George EF (2008) Plant growth regulators II: cytokinins, their analogues and antagonists. In: George EF, Hall MA, De Klerk G-J (eds) Plant propagation by tissue culture. Part I: The technology, 3rd edn. Springer, pp 205–226

  • Vijay LS, Padmakar AS, Vikas VV, Manisha MB, Ganesh RS (2008) RP-LC determination of rutin in Amaranthus spinosus Linn. whole powder. Chromatographia 67:189–191

    Article  Google Scholar 

  • Ziv M (2000) Bioreactor technology for plant micropropagation. In: Janick J (ed) Horticultural reviews, vol 24. Wiley and Sons, pp 1–30

Download references

Acknowledgments

The authors acknowledge financial support by the College of Food and Agricultural Research Centre, Deanship of Scientific Research, King Saud University.

Author information

Authors and Affiliations

  1. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    Mahmoud A.-H. Mohamed

  2. Department of Pharmacognosy, College Pharmacy, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    Taghreed Ibrahim

  3. Department of Horticulture, Faculty of Agriculture, Minia University, Al Minia, Egypt

    Mahmoud A.-H. Mohamed

  4. Department of Pharmacognosy, College Pharmacy, Cairo University, Giza, Egypt

    Taghreed Ibrahim

Authors
  1. Mahmoud A.-H. Mohamed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Taghreed Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mahmoud A.-H. Mohamed.

Additional information

Communicated by M. Stobiecki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mohamed, M.AH., Ibrahim, T. In vitro mass production of Ruta gravoelens L. for secondary products production. Acta Physiol Plant 33, 1945–1951 (2011). https://doi.org/10.1007/s11738-011-0743-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-011-0743-7

Keywords

Search

Navigation