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Effect of 24-epibrassinolide on oxidative stress markers induced by nickel-ion in Raphanus sativus L.

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Abstract

The present study illustrates the effect of 24-epibrassinolide (24-EBL) on morphological and biochemical parameters in radish (Raphanus sativus L.) seedlings grown under nickel (Ni) ion stress. The radish seeds pre-soaked in different concentrations of 24-EBL were sown in petridishes containing various concentrations of heavy metal (Ni).Observations were made on root/shoot length, fresh biomass, activities of antioxidant enzymes (ascorbate peroxidase, superoxide dismutase, catalase, monodehydroascorbate reductase, dehydroascorbate reductase, guaiacol peroxidase and glutathione reductase), lipid peroxidation, proline and protein content in 7-day-old Ni-stressed radish seedlings. Results indicate that seeds presoaked with 24-EBL reduced the impact of Ni-stress which was evident by assessing the morphological parameters, protein content and antioxidant enzyme activities. It was also observed that 24-EBL reduced the toxicity of heavy metal by influencing proline and malondialdehyde (MDA) content. The present study lays a foundation for understanding the role of 24-EBL in heavy metal stress amelioration, particularly in food crop. Analysis of behaviour of antioxidant enzymes will play a critical role in understanding the stress networking, further filling the knowledge gap on the subject.

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Acknowledgments

Financial assistance from Department of Science and Technology (DST), Ministry of Science & Technology, Government of India, New Delhi, India, is duly acknowledged.

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Authors and Affiliations

  1. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Indu Sharma & Renu Bhardwaj

  2. Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Pratap Kumar Pati

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  1. Indu Sharma
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  2. Pratap Kumar Pati
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Correspondence to Renu Bhardwaj.

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Communicated by S. Lewak.

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Sharma, I., Pati, P.K. & Bhardwaj, R. Effect of 24-epibrassinolide on oxidative stress markers induced by nickel-ion in Raphanus sativus L.. Acta Physiol Plant 33, 1723–1735 (2011). https://doi.org/10.1007/s11738-010-0709-1

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