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Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

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Abstract

This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige–Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5.0 μM) and kinetin (KIN; 5.0 μM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (μ) of 0.14 d−1 and doubling time (t d) of 6.6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactan-proteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.

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Acknowledgments

This work was financed by grant SIP 20100401 from IPN. Trejo-Espino JL is indebted to Instituto Politécnico Nacional (IPN) and CONACYT for the doctoral fellowship awarded.

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Authors and Affiliations

  1. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, CP 09340, México, DF, México

    J. L. Trejo-Espino & F. Cruz-Sosa

  2. Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, PO Box 24, CP 62730, Yautepec, Morelos, México

    J. L. Trejo-Espino & M. Rodríguez-Monroy

  3. Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, CP 09340, México, DF, México

    E. J. Vernon-Carter

Authors
  1. J. L. Trejo-Espino
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  2. M. Rodríguez-Monroy
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  3. E. J. Vernon-Carter
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  4. F. Cruz-Sosa
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Corresponding author

Correspondence to F. Cruz-Sosa.

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Communicated by B. Borkowska.

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Trejo-Espino, J.L., Rodríguez-Monroy, M., Vernon-Carter, E.J. et al. Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production. Acta Physiol Plant 33, 1687–1695 (2011). https://doi.org/10.1007/s11738-010-0705-5

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  • DOI: https://doi.org/10.1007/s11738-010-0705-5

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