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Over-expression of the Arabidopsis Na+/H+ antiporter gene in Populus deltoides CL × P. euramericana CL “NL895” enhances its salt tolerance

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Abstract

Soil salinity is a serious problem worldwide. It is necessary to improve the salt tolerance of plants to avoid the progressive deterioration of saline soil. We showed that the over-expression of AtNHX1 improves salt tolerance in a transgenic poplar (Populus deltoides CL × P. euramericana CL “NL895”) under mannose selection. Four transgenic poplar plants were obtained. Southern blot analysis showed that the pmi gene had integrated into the genome of the poplar. RT-PCR confirmed that AtNHX1 could be expressed normally in the transgenic plants. When tested for salt tolerance by NaCl stress, we measured a 100% increase in Na+ content in the three transgenic lines (T18, T50, T98) significantly higher than the 33% increase seen in wild-type plants. The chlorophyll content of the transgenic plants was not altered significantly, while the chlorophyll content in the control plants showed a small decrease. MDA content was decreased in the transgenic plants. These results show that the AtNHX1 gene may enhance salt tolerance due to increased vacuolar compartmentalization of sodium ions.

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Acknowledgments

The authors thank Dr. Roberto A. Gaxiola at the University of Connecticut for kindly providing AtNHX1 cDNA. This work was supported by the National Natural Science Foundation of China (30972340) and Zhejiang Provincial Natural Science Foundation of China under Grant No. R3090070.

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Authors and Affiliations

  1. Key Lab of Tree Genomics, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, 311400, Zhejiang, China

    Guirong Qiao, Renying Zhuo, Mingying Liu, Jing Jiang, Haiying Li, Wenmin Qiu, Luanyin Pan, Sheng lin & Xiaoguo Zhang

  2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, Zhejiang, China

    Zongxiu Sun

  3. Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Sheng lin

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  1. Guirong Qiao
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  2. Renying Zhuo
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  3. Mingying Liu
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  4. Jing Jiang
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  5. Haiying Li
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  6. Wenmin Qiu
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  7. Luanyin Pan
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  8. Sheng lin
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  9. Xiaoguo Zhang
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  10. Zongxiu Sun
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Corresponding author

Correspondence to Renying Zhuo.

Additional information

Communicated by S. Abe.

The authors G. Qiao, M. Liu and J. Jiang contributed equally to this work.

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Qiao, G., Zhuo, R., Liu, M. et al. Over-expression of the Arabidopsis Na+/H+ antiporter gene in Populus deltoides CL × P. euramericana CL “NL895” enhances its salt tolerance. Acta Physiol Plant 33, 691–696 (2011). https://doi.org/10.1007/s11738-010-0591-x

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  • DOI: https://doi.org/10.1007/s11738-010-0591-x

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