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Differential responses of pea seedlings to indole acetic acid under manganese toxicity

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Abstract

Present study showed the responses of pea seedlings to exogenous indole acetic acid (IAA; 10 and 100 μM) application under manganese (Mn; 50, 100 and 250 μM) toxicity. Manganese and 100 μM IAA alone as well as in combination decreased growth of pea seedlings compared to control. Moreover, some parameters of oxidative stress—hydrogen peroxide (H2O2) and malondialdehyde (MDA) were also increased by single and combined treatments of Mn and 100 μM IAA compared to control. In contrast, addition of 10 μM IAA together with Mn, alleviated Mn toxicity symptoms and promoted growth led to the decrease in H2O2 and MDA levels compared to Mn treatments alone. Under single and combined treatments of Mn and 100 μM IAA, catalase activity decreased while superoxide dismutase and ascorbate peroxidase activities increased and glutathione reductase and dehydroascorbate reductase exhibited differential responses. However, addition of 10 μM IAA together with Mn, increased activities of studied enzymatic antioxidants. Root and shoot reduced ascorbate (AA) and reduced glutathione (GSH) and, their reduced/oxidized ratios decreased while dehydroascorbate (DHA) and oxidized glutathione (GSSG) contents increased compared to control following single and combined treatments of Mn and 100 μM IAA. However, supply of 10 μM IAA together with Mn, increased AA and GSH, and their reduced/oxidized ratios in root and shoot compared to Mn treatments alone. This study thus suggests that 10 μM of IAA was able to increase Mn tolerance in pea seedlings under Mn toxicity while opposite was noticed for 100 μM IAA.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

DHAR:

Dehydroascorbate reductase

FM:

Fresh mass

GR:

Glutathione reductase

SOD:

Superoxide dismutase

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Acknowledgments

The authors are thankful to The Head, Department of Plant Science, M.J.P. Rohilkhand University, Bareilly and The Head, Department of Botany, University of Allahabad, Allahabad for providing necessary laboratory facilities during research work. The authors are also thankful to UGC, New Delhi for providing financial support to carry out this work.

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Authors and Affiliations

  1. Department of Plant Science, M.J.P. Rohilkhand University, Bareilly, 243006, India

    Savita Gangwar & Jagat Narayan Maurya

  2. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, India

    Vijay Pratap Singh & Sheo Mohan Prasad

Authors
  1. Savita Gangwar
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  2. Vijay Pratap Singh
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  3. Sheo Mohan Prasad
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  4. Jagat Narayan Maurya
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Correspondence to Jagat Narayan Maurya.

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Communicated by J. Ueda.

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Gangwar, S., Singh, V.P., Prasad, S.M. et al. Differential responses of pea seedlings to indole acetic acid under manganese toxicity. Acta Physiol Plant 33, 451–462 (2011). https://doi.org/10.1007/s11738-010-0565-z

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  • DOI: https://doi.org/10.1007/s11738-010-0565-z

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