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Effect of methylglyoxal bis-(guanylhydrazone) on polyamine and ethylene biosynthesis of apple fruit after harvest

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Abstract

Polyamine and ethylene both play important roles in fruit ripening, whose biosynthetic pathways share a common substrate, S-adenosylmethionine (SAM). To unravel the interrelationship between polyamine and ethylene, their metabolism and expression of relevant genes were investigated in apple fruit (Malus domestica Borkh.) treated with methylglyoxal bis-(guanylhydrazone) (MGBG). The MGBG-treated fruit had higher ethylene production until 16 days after treatment (DAT) with preceding accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC) than control fruit and then decreased to nearly the same level as control. Ethylene promotion at the early stage by MGBG was accompanied by increased expression of apple ACC synthase (Md-ACS1) and ACC oxidase (MdACO). The expression of apple SAM synthase (MdSAMS) in MGBG-treated fruit was slightly higher than that in control. On the other hand, significant changes in free polyamine titers were observed at some stages, but the changes did not show consistent trends. Based on these observations, possible relationship between polyamine and ethylene pathways was discussed.

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Fig. 1
Fig. 2

Abbreviations

1-MCP:

1-Methylcyclopropene

ACC:

1-Aminocyclopropane-1-carboxylic acid

ACO:

ACC oxidase

ACS:

ACC synthase

AVG:

Aminoethoxyvinylglicine

DAT:

Days after treatment

MGBG:

Methylglyoxal bis-(guanylhydrazone)

Put:

Putrescine

SAM:

S-Adenosylmethionine

SAMDC:

S-Adenosylmethionine decarboxylase

SAMS:

SAM synthase

Spd:

Spermidine

Spm:

Spermine

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Acknowledgment

We thank Dr. R.C. Sotto (University of the Philippines, Los Banos) for her critical reading of the manuscript. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan and from the Japan Society for Promotion of Science (JSPS).

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Authors and Affiliations

  1. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China

    X. M. Pang

  2. National Institute of Fruit Tree Science, Tsukuba, Ibaraki, 305-8605, Japan

    X. M. Pang, Y. Ban & T. Moriguchi

  3. Mie University, Tsu, Mie, 514-8507, Japan

    K. Nada & T. Kurosawa

  4. University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Y. Ban & T. Moriguchi

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  1. X. M. Pang
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  2. K. Nada
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  3. T. Kurosawa
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  4. Y. Ban
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  5. T. Moriguchi
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Corresponding author

Correspondence to T. Moriguchi.

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Communicated by J.-H. Liu.

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Pang, X.M., Nada, K., Kurosawa, T. et al. Effect of methylglyoxal bis-(guanylhydrazone) on polyamine and ethylene biosynthesis of apple fruit after harvest. Acta Physiol Plant 32, 1005–1010 (2010). https://doi.org/10.1007/s11738-010-0473-2

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  • DOI: https://doi.org/10.1007/s11738-010-0473-2

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