Abstract
In order to understand how the nutrient elements were taken up during the cell growth as well as the production of metabolites, it was quite necessary to identify the dynamic change of metabolites and nutrients in suspension cells of Panax quinquefolium in bioreactor. In this study, dynamic accumulation of biomass and ginsenosides Re, Rb1 and polysaccharide as well as major nutrients consumption in cell suspension culture of P. quinquefolium in a 5-L stirred tank bioreactor were investigated. The dry cell weight and the contents of ginsenosides Re, Rb1 and polysaccharide reached the maximum peak simultaneously on about 21 days and the results showed that cell growth and metabolites synthesis related to nutrients consumption. For this reason, we supposed that the contents of metabolites can be increased through added nutrient at the right moment. These results provided theory reference for two-stage or continuous perfusion culture in suspension cells of P. quinquefolium in bioreactor.
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References
Ali MB, Yu KW, Hahn EJ, Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity. Ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng C.A. Mayer and Panax quinquefolium L. in bioreactor subjected to methyl jasmonate stress. Plant Sci 169:83–92
Daie J, Chin CK, Pitcher L (1987) Differential rates of sucrose and hexose transport by asparagus cell cultures. Plant Sci 53:101–107
Dantas AK, Majada JP, Fernandez B, Canal MJ (2001) Mineral nutrition in carnation tissue cultures under different ventilation conditions. Plant Growth Regul 33:237–243
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Gao WY, Jia W, Duan HQ, Xiao PG (2003) Industrialization of medicinal plant tissue culture. Chin J Chin Mater Med 28:385–390
Hecht U, Mohr H (1990) Factors controlling nitrate and ammonium accumulation in mustard (Sinapis alba) seedlings. Physiol Plant 78:379–387
Huang YC, Chen CT, Chen SC, Lai PH, Liang HC, Chang Y, Yu LC, Sung HW (2005) A natural compound (ginsenoside Re) isolated from Panax ginseng as a novel angiogenic agent for tissue regeneration. Pharm Res 22:636–646
Jeong CS, Chakrabarty D, Hahn EJ, Lee HL, Paek KY (2006) Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochem Eng J 27:252–263
Jiang ST, Wei M, Luo JP (2006) Effect of phosphate on growth and polysaccharide production by suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Chin J Biotechnol 22:716
Kanabus J, Bressan ARA, Carpita NC (1986) Carbon assimilation in carrot cells in liquid culture. Plant Physiol 82:363–368
Kevers C, Jacques P, Thonat P, Gasphar TH (1999) In vitro root cultures of Panax ginseng and P. quinquefolium. Plant Growth Regul 27:173–178
Liu D, Li B, Liu Y, Attele AS, Kyle JW, Yuan CS (2001) Voltage dependent inhibition of brain Na(+) channels by American ginseng. Eur J Pharmacol 413:47–54
Mathur A, Mathur AK, Sangwan RS, Gangwar A, Uniyal GC (2003) Differential morphogenetic responses, ginsenoside metabolism and RAPD patterns of three Panax species. Genet Resour Crop Evol 50:245–252
Mehendale S, Aung H, Wang A, Yin JJ, Wang CZ, Xie JT, Yuan CS (2005) American ginseng berry extract and ginsenoside Re attenuate cisplatin-induced kaolin intake in rats. Cancer Chemother Pharmacol 56:63–69
Morard P, Fulcheri C, Henry M (1998) Kinetics of mineral nutrient uptake by Saponaria officinalis L. suspension cell cultures in different media. Plant Cell Rep 18:260–265
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Nocerino E, Amato M, Izzo AA (2000) The aphrodisiac and adaptogenic properties of ginseng. Fitoterapia 71:s1–s5
Schlatmann JE, Moreno PRH, Selles M, Vinke JL, Hoopen HJG, Verpoorte R, Heijnen JJ (1995) Two-stage batch process for the production of ajmalicine by Catharanthus roseus: the link between growth and production stage. Biotechnol Bioeng 47:53–59
Shin KS, Chakrabarty D, Ko JY, Han SS, Paek KY (2002) Sucrose utilization and mineral nutrient uptake during hairy root growth of red beet (Beta vulgaris L.) in liquid culture. Plant Growth Regul 39:187–193
Sivakumar G, Yu KW, Paek KY (2005) Production of biomass and ginsenosides from adventitious roots of panax ginseng in bioreactor cultures. Eng Life Sci 5:333–342
Wang Z, Li M, Wu WK, Tan HM, Geng DF (2008) Ginsenoside Rb1 preconditioning protects against myocardial infarction after regional ischemia and reperfusion by activation of phosphatidylinositol-3-kinase signal transduction. Cardiovasc Drugs Ther 22:443–452
Weatherburn MW (1967) Phenol-hypochlorite reaction for determination of ammonium. Anal Chem 39:971–974
Zhang YH, Zhong JJ, Yu JT (1995) Effect of osmotic pressure on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnol Lett 17:1347–1350
Zhang YH, Zhong JJ, Yu JT (1996) Effect of nitrogen source on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnol Prog 12:567–571
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This research was funded by China National Center for Biotechnology Development (2006BAI06A16-02).
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Communicated by J. van Huylenbroeck.
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Wang, J., Gao, WY., Zhang, J. et al. Dynamic change of metabolites and nutrients in suspension cells of Panax Quinquefolium L. in bioreactor. Acta Physiol Plant 32, 463–467 (2010). https://doi.org/10.1007/s11738-009-0422-0
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DOI: https://doi.org/10.1007/s11738-009-0422-0