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Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential

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Abstract

Cucumber (Cucumis sativus L.) cytokinin-independent embryogenic cell suspension cultures were derived and maintained for more than 3.5 years without losing the embryogenic potential. The preparation and the characteristics of the cucumber embryogenic cell suspension possess many similarities to that of carrot. The cultures were induced from hypocotyl explants of in vitro grown cucumber plants in liquid MS media containing 2,4-dichlorophenoxyacetic acid as the sole growth regulator during 6 weeks and they contained a heterogeneous array of several different types of single cells and cell clusters (PEMs). The established cell suspensions were subcultured in 1-week interval, while the inoculation density was optimized to 2.0 × 105 cells ml−1 using cell viability as a marker. Somatic embryos were obtained after the transfer of the proembryogenic masses to a hormone-free semisolid MS medium with a frequency of 388 ± 57 somatic embryos per 1 ml of packed cell volume of the established cucumber embryogenic culture within 7 days. The frequency of normal somatic embryos with two cotyledons was found to be 78%. Such embryos possessed the potential of spontaneous maturation and the embryo conversion rates were 87%. The yield of normally growing plants was much higher compared with that previously described for cucumber systems. Somatic embryo-derived plants were successfully transferred to the greenhouse where they flowered and fruited.

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Acknowledgment

The presented work was funded by the Czech Ministry of Education, Project Nr. 10 MSM432100001.

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, 613 00, Brno, Czech Republic

    Lenka Andrýsková, Vilém Reinöhl, Marek Klemš & Stanislav Procházka

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  1. Lenka Andrýsková
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  2. Vilém Reinöhl
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  3. Marek Klemš
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  4. Stanislav Procházka
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Corresponding author

Correspondence to Vilém Reinöhl.

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Communicated by E. Lojkowska.

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Andrýsková, L., Reinöhl, V., Klemš, M. et al. Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential. Acta Physiol Plant 31, 675–681 (2009). https://doi.org/10.1007/s11738-009-0277-4

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  • DOI: https://doi.org/10.1007/s11738-009-0277-4

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