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Inoculum size and auxin concentration influence the growth of adventitious roots and accumulation of ginsenosides in suspension cultures of ginseng (Panax ginseng C.A. Meyer)

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Abstract

The effect of the root-inoculum size and axuin concentration on growth of adventitious roots and accumulation of ginsenosides were studied during suspension cultures of ginseng (Panax ginseng C.A. Meyer). Of the various concentrations of indole-3-butyric acid (IBA) and γ-naphthaleneacetic acid (NAA) used as supplementary growth regulators along with Murashige and Skoog medium, 25 μM IBA was found suitable for lateral root induction and growth, as well as accumulation of ginsenosides. Inoculum size of 5 g L−1 was found suitable for optimal biomass (10.5 g L−1 dry biomass) and ginsenosides (5.4 mg g−1 DW) accumulation. Of the various length of root inocula tested (chopped to 1–3, 4–6, 7–10 mm and un-chopped), root inocula of 7–10 mm was found suitable for biomass and ginsenoside accumulation.

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Acknowledgments

This work was financially supported by the ministry of Education and Human Resources Development, the ministry of Commerce, Industry and Energy, and the ministry of labor, Govt. of Republic Korea.

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, South Korea

    C. S. Jeong, H. N. Murthy, E. J. Hahn, H. L. Lee & K. Y. Paek

  2. Department of Botany, Karnatak University, Dharwad, 580 003, India

    H. N. Murthy

  3. Department of Urology, College of Medicine, Kyung Hee University, Seoul, South Korea

    H. L. Lee

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  1. C. S. Jeong
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  2. H. N. Murthy
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  3. E. J. Hahn
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  4. H. L. Lee
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  5. K. Y. Paek
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Corresponding author

Correspondence to H. N. Murthy.

Additional information

Communicated by M. Horbowicz.

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Jeong, C.S., Murthy, H.N., Hahn, E.J. et al. Inoculum size and auxin concentration influence the growth of adventitious roots and accumulation of ginsenosides in suspension cultures of ginseng (Panax ginseng C.A. Meyer). Acta Physiol Plant 31, 219–222 (2009). https://doi.org/10.1007/s11738-008-0206-y

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  • DOI: https://doi.org/10.1007/s11738-008-0206-y

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