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Increased activity of only an individual non-regulated enzyme fructose-1,6-bisphosphate aldolase in Anabaena sp. strain PCC 7120 stimulates photosynthetic yield

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Abstract

The goal of this study was to investigate the contribution of increased activity of individual non-regulated enzymes in the Calvin cycle to improve photosynthetic yield. Two non-regulated enzymes, rice fructose-1,6-bisphosphate aldolase (FBA) and spinach triosephosphate isomerase (TPI), were individually cloned and overexpressed in the cyanobacterium Anabaena sp. strain PCC 7120 cells. The enzyme activity and the photosynthetic yield, as reflected by the cell growth rate, photosynthetic oxygen evolution and dry cellular weight, were measured and compared between the wild-type and transgenic cells harboring either FBA or TPI. Though the activity of these two individual non-regulated enzymes was similarly increased in the corresponding transgenic cells, the contributions of each enzyme on the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), reflected by the levels of Rubisco large subunit, and the photosynthetic yield were different. Transgenic cells, carrying FBA, showed an evident increase in Rubisco amount and photosynthetic yield, while there was no increase in cells harboring TPI. This indicates that the contributions of non-regulated enzymes in the Calvin cycle on photosynthetic yield differed and firstly reveals that increased activity of only a single non-regulated enzyme in transgenic cells markedly improves the photosynthetic yield via stimulating the amount of Rubisco and consequently accelerating the ribulose-1,5-bisphosphate (RuBP) regeneration rate.

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Abbreviations

CS:

Control strain harboring pDC-08

FBA:

Fructose-1,6-bisphosphate aldolase

FBPase:

Fructose-1,6-bisphosphatase

RbcL:

Rubisco large subunit

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

RuBP:

Ribulose-1,5-bisphosphate

SBPase:

Sedoheptulose-1,7-bisphosphatase

TPI:

Triosephosphate isomerase

TS1:

Transgenic strain harboring pDC-FBA

TS2:

Transgenic strain harboring pDC-TPI

WT:

Wild-type

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Acknowledgments

The authors are deeply grateful to Prof. H. Chen (Institute of Organic Chemistry, Chinese Academy of Sciences) for providing the pDCFAT plasmid, Prof. C. P. Wolk (Michigan State University) for providing the pDC-08, RP4 and pRL623 plasmids, and Prof. G. Chen (Institute of Plant Physiology and Ecology, Chinese Academy of Sciences) for providing the antibody against RbcL. This work was partially supported by the National Natural Science Foundation of China (No. 30770175), the Shanghai Natural Science Foundation (No. 07ZR14086), the Innovation Program of Shanghai Municipal Education Commission (Nos. 05ZZ15 and 08ZZ67), and the Key Fundamental Project of Shanghai (No. 06JC14091).

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Authors and Affiliations

  1. College of Life and Environment Sciences, Shanghai Normal University, 200234, Shanghai, China

    Weimin Ma, Lanzhen Wei, Zongjuan Long, Liping Chen & Quanxi Wang

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  1. Weimin Ma
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  2. Lanzhen Wei
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  3. Zongjuan Long
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  4. Liping Chen
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  5. Quanxi Wang
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Correspondence to Weimin Ma or Quanxi Wang.

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Communicated by S. Lewak.

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Ma, W., Wei, L., Long, Z. et al. Increased activity of only an individual non-regulated enzyme fructose-1,6-bisphosphate aldolase in Anabaena sp. strain PCC 7120 stimulates photosynthetic yield. Acta Physiol Plant 30, 897–904 (2008). https://doi.org/10.1007/s11738-008-0182-2

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  • DOI: https://doi.org/10.1007/s11738-008-0182-2

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