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Exogenous expression of the wheat chloroplastic fructose-1,6-bisphosphatase gene enhances photosynthesis in the transgenic cyanobacterium, Anabaena PCC7120

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Abstract

The paper reports a study on the genetic regulation of photosynthesis by introducing the gene encoding wheat chloroplastic fructose-1,6-bisphosphatase (FBPase) into the cyanobacterium Anabaena PCC7120. The gene was RT-PCR amplified from wheat and modified by replacement of the 5′-terminal encoding sequence with optimal and A/T-rich codons to promote prokaryotic expression. The resultant FBPase gene was ligated downstream of the strong promoter, PpsbA of expression vector pRL-439, then inserted into of shuttle vector pDC-08. The resulting shuttle expression vector (pDC-fbp) was transferred into the filamentous, heterocystour cyanobacterium, Anabaena PCC7120, by the tri-parental conjugation transfer method. Protein expression of FBPase in the transgenic Anabaena was 126.5% higher than in wild type cells, and the enzyme activity of transgenic cells was 1.41-fold higher than that of wild type cells. Under atmospheric conditions of 360 μmol mol−1 CO2, Anabaena cells overexpressing the FBPase gene further showed increases in net photosynthesis (117.2%) and true photosynthesis (122.5%) as compared to wild type cells. In addition, transgenic Anabaena grew faster and contained more Chl a than did wild type cells. Together, these results indicate that introduction of the wheat chloroplastic FBPase gene into Anabaena increase photosynthesis and cell growth; furthermore, these trends were more evident under stress condition (higher CO2 concentration). This is the first report of enhanced photosynthesis in cyanobacteria expressing genes from higher plants.

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Abbreviations

FBPase:

fructose-1,6-bisphosphatase

SBPase:

sedoheptulose-1,7-bisphosphatase

FBP/SBPase:

fructose-1,6/sedoheptulose-1,7-bisphosphatase

CCM:

CO2-concentrating mechanism

PAGE:

polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Department of Biology, Shanghai Normal University, Shanghai, People’s Republic of China

    Weimin Ma, Dingji Shi, Quanxi Wang & Lanzhen Wei

  2. Laboratory of Photosynthesis, Institute of Botany, Chinese Academy of Science, Beijing, People’s Republic of China

    Weimin Ma & Dingji Shi

  3. State Key Laboratory of Bio-organic and Natural Products Chemistry, Institute of Organic Chemistry, Chinese Academy of Science, Shanghai, People’s Republic of China

    Haibao Chen

  4. National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, People’s Republic of China

    Weimin Ma

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  1. Weimin Ma
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  2. Dingji Shi
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  3. Quanxi Wang
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  4. Lanzhen Wei
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Correspondence to Dingji Shi.

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Ma, W., Shi, D., Wang, Q. et al. Exogenous expression of the wheat chloroplastic fructose-1,6-bisphosphatase gene enhances photosynthesis in the transgenic cyanobacterium, Anabaena PCC7120. J Appl Phycol 17, 273–280 (2005). https://doi.org/10.1007/s10811-005-4850-y

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  • DOI: https://doi.org/10.1007/s10811-005-4850-y

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