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Red cabbage extract limits copper stress injury in meristematic cells of Vicia faba

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Abstract

Natural phenolic compounds (phenolic acids, flavonoids, tannins, lignans) present in food of plant origin are in the focus of interest due to their prevalence, properties and biological activity. The aim of the presented work was to investigate antioxidant and antigenotoxic effects of the anthocyanin-rich extract from red cabbage leaves (Brassica oleracea rubrum) on the changes induced by toxic Cu2+ concentrations. MeOH extract from red cabbage containing anthocyanin (ATH) and phenolic acid derivatives exhibited strong antioxidant properties. Cu2+ decreased mitotic index (MI) and inhibited proliferative activity of Vicia faba root meristematic cells. The morphology of mitotic chromosomes was changed; “erosion” and pulverization might result from Cu2+ high-cytotoxicity. Numerous micronuclei, chromatid bridges and lagging/lost chromosomes were found in the meristematic cells of V. faba, which indicate the clastogenic effect of Cu2+. The application of the ATH-rich extract lowered the number of disturbances induced by Cu2+. The positive role of the ATH-rich red cabbage extract will be discussed.

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Abbreviations

ATH:

Anthocyanins

DM:

Extract dry matter

FRAP:

Ferric reducing ability of plazma

FW:

Fresh weight

MI:

Mitotic index

MN:

Miconuclei

PhC:

Phenolic compounds

ROS:

Reactive oxygen species

TE:

Trolox equivalents

TAC:

Total antioxidant capacity

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Acknowledgments

This research was supported by State Committee for Scientific Research, grant 2PO4G 044 26 in years 2004–06.

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Authors and Affiliations

  1. Department of Ecophysiology and Plant Development, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland

    Małgorzata M. Posmyk & Krystyna M. Janas

  2. Department of Cytogenetics and Plant Molecular Biology, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland

    Renata Kontek

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  1. Małgorzata M. Posmyk
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Correspondence to Małgorzata M. Posmyk.

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Communicated by S. Lewak.

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Posmyk, M.M., Kontek, R. & Janas, K.M. Red cabbage extract limits copper stress injury in meristematic cells of Vicia faba . Acta Physiol Plant 30, 481–491 (2008). https://doi.org/10.1007/s11738-008-0145-7

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  • DOI: https://doi.org/10.1007/s11738-008-0145-7

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