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Breaking dormancy of Stylosanthes humilis seeds with low pH solutions

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Abstract

An acidic condition (low pH) of the germination media promoted dormancy breakage of scarified seeds of Townsville stylo (Stylosanthes humilis H.B.K.), an annual tropical forage legume, whether produced by either an unbuffered (HCl-KOH) or a buffered (phthalate, McIlvaine) medium. Except for aminooxyacetic acid, all ethylene biosynthesis inhibitors tested and supplied with the low pH solutions decreased germination to variable extents. Low pH-stimulated dormant seeds produced ethylene 4-fold as much than untreated seeds. Production of ethylene by seeds treated with high pH solutions, which did not affect their dormant state, was also very low.

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Abbreviations

ABA:

abscisic acid

ACC:

1-aminocyclopropane-1-carboxylic acid

AIB:

α-aminoisobutyric acid

AOA:

aminooxyacetic acid

ASA:

acetylsalicylic acid

AVG:

2-aminoethoxyvinylglicine

CEPA:

2-chloroethylphosphonic acid

SA:

salicylic acid

References

  • Adkins S.W., Ross J.D. 1981. Studies in wild oat seed dormancy I. The role of ethylene in dormancy breakage and germination of wild oat seeds (Avena fatua L.). Plant Physiol., 67: 358–362.

    PubMed  CAS  Google Scholar 

  • Adkins S.W., Simpson G.M., Naylor J.M. 1985. The physiological basis of seed dormancy in Avena fatua VII. Action of organic acids and pH. Physiol. Plant., 65: 310–316.

    Article  CAS  Google Scholar 

  • Argel R.J., Humphreys L.R. 1983. Environment effects on seed development and hardseededness in Sthylosanthes hamata cv. Verano I. Temperature. Aust. J. Agr. Res., 34: 261–270.

    Article  Google Scholar 

  • Barros R.S., Freitas A.W.P. 2000. Stimulation of germination of dormant seeds of Townsville stylo by selenomethionine. Seed Sci. Technol., 28: 241–247.

    Google Scholar 

  • Barros R.S., Freitas A.W.P. 2001. Selenomethionone as a dormancy-breaking agent in seeds of Stylosanthes humilis. Acta Physiol. Plant., 23: 279–284.

    CAS  Google Scholar 

  • Burin M.E., Barros R.S., Rena A.B. 1987. Chemical regulation of endogenous dormancy in seeds of Stylosanthes humilis HBK. Turrialba, 37: 281–285.

    CAS  Google Scholar 

  • Cameron D.F. 1967. Hardseededness and seed dormancy of Townsville lucerne (Stylosanthes humilis) selections. Aust. J. Exp. Agr. Anim. Husb., 7: 237–240.

    Article  Google Scholar 

  • Carvalho L.J.C.B., Mosquim P.R., Rena A.B. 1988. Resposta ao alumínio e fósforo por plantas de Stylosanthes humilis Sw coletadas em solo de cerrado com diferentes características químicas. In: EMBRAPACPAC (ed), VI Simpósio sobre cerrado e savanas: alimento e energia. Planaltina, DF, EMBRAPA, pp. 423–443.

    Google Scholar 

  • Cohn M.A. 1989. Factors influencing the efficacy of dormancy-breaking chemicals. In: Taylorson, R.B. (ed) Recent advances in development and germination of seeds. New York, Plenum Press, pp. 261–267.

    Google Scholar 

  • Cohn M.A., Chiles L.A., Hughes J.A., Boullion K.J. 1987. Seed dormancy in red rice VI. Monocarboxylic acids: a new class of pH-dependent germination stimulants. Plant Physiol., 84: 716–719.

    Article  PubMed  CAS  Google Scholar 

  • Delatorre C.A. 1994. Regulaç o n o-específica da germinaç o de sementes de Stylosanthes humilis HBK. MSc. Thesis, Viçosa, UFV.

  • Delatorre C.A., Barros R.S. 1996. Germination of dormant seeds of Stylosanthes humilis as related to heavy metal ions. Biol. Plant., 38: 269–274.

    CAS  Google Scholar 

  • Footitt S., Cohn M.A. 1992. Seed dormancy in red rice VII. Embryo acidification during dormancy-breaking and subsequent germination. Plant Physiol., 100: 1196–1202.

    PubMed  CAS  Google Scholar 

  • Gardener C.J. 1975. Mechanisms regulating germination in seeds of Stylosanthes. Aust. J. Agr. Res., 26: 281–294.

    Article  Google Scholar 

  • Holm A.M. 1972. The effect of high temperature pretreatment on germination of Townsville stylo seed material. Aust. J. Exp. Agr. Anim. Husb., 13: 190–192.

    Article  Google Scholar 

  • McIvor F.G. 1976. Germination characteristics of seven Stylosanthes species. Aust. J. Exp. Agr. Anim. Husb., 16: 723–728.

    Article  Google Scholar 

  • Ribeiro D.M. 2003. Germinaç o de sementes dormentes de Stylosanthes humilis promovida pelo acúmulo de etileno em ambiente fechado. MSc. Thesis, Viçosa, UFV.

  • Roberts E.H. 1963. The effect of inorganic ions on dormancy in rice seeds. Physiol. Plant., 16: 732–744.

    Article  CAS  Google Scholar 

  • Saltveit- M.E. Jr, Yang S.F. 1987. Ethylene. In: Rivier L., Crozier A. (eds) Principles and practice of plant hormone analysis, Vol 2. Academic Press, London. Pp. 367–401.

    Google Scholar 

  • Scott A.J., Knott M. 1974. A cluster analysis method for grouping means in the analysis of variance. Biometrics, 30: 507–512.

    Article  Google Scholar 

  • Simpson G.M. 1965. Dormancy studies in seed of Avena fatua. The role of gibberellin in embryo dormancy. Can. J. Bot., 43: 793–816.

    Article  CAS  Google Scholar 

  • Vieira H.D., Barros R.S. 1994. Responses of seed of Stylosanthes humilis to germination regulators. Physiol. Plant., 92: 17–20.

    Article  CAS  Google Scholar 

  • Vieira H.D., Silva R.F., Barros R.S. 1999. pH and gibberellic acid overcome dormancy of seeds of Brachiaria brizantha cv. Marandu. Rev. Bras. Fisiol. Veg., 11: 51–54.

    CAS  Google Scholar 

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Author notes

  1. Claudinéia R. Pelacani

    Present address: Depto Biologia, Univ. Estadual de Feira de Santana, 44031-460, Feira de Santana, BA, Brazil

  2. Renita B. C. Frigeri

    Present address: Campus Vilhena, Univ. Federal de Rondônia, 78995-000, Vilhena, RO, Brasil

Authors and Affiliations

  1. Depto Biologia Vegetal, Univ. Federal de Viçosa, 36571-000, Viçosa, MG, Brazil

    Claudinéia R. Pelacani, Raimundo S. Barros, Dimas M. Ribeiro & Renita B. C. Frigeri

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  1. Claudinéia R. Pelacani
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  2. Raimundo S. Barros
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  3. Dimas M. Ribeiro
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  4. Renita B. C. Frigeri
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Correspondence to Raimundo S. Barros.

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Pelacani, C.R., Barros, R.S., Ribeiro, D.M. et al. Breaking dormancy of Stylosanthes humilis seeds with low pH solutions. Acta Physiol Plant 27, 387–394 (2005). https://doi.org/10.1007/s11738-005-0016-4

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  • DOI: https://doi.org/10.1007/s11738-005-0016-4

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