Abstract
Phaseolus vulgaris seedlings were grown in light with or without chromium. Changes in cell wall components i.e. pectic polysaccharides and xyloglucan contents were looked into during cell elongation, by two different methods in order to find the most suitable method for isolation of cell wall polysaccharides. The first method was short and easy. It made use of organic solvents for preparation of cell wall components and ammonium oxalate and oxalic acid buffer and high temperature for extracting pectic polysaccharides; 0.7 M and 4.3 M KOH was used for extracting low and high molecular weight xyloglucans respectively. On the other hand, in the second method, cell wall components were fractionated by sequential treatments with different inorganic solvents, chelating agents, sodium lauryl sulphate, etc. KOH (1 M and 4 M) was used for extracting xyloglucans. The advantage of using the second method for extracting cell wall polysaccharides especially pectic polysaccharides is discussed.
Similar content being viewed by others
Abbreviations
- EDTA:
-
ethylene diammine tetra acetic acid
- DMSO:
-
dimethyl sulphoxide
- SLS:
-
sodium lauryl sulphate
References
Bagatharia S.B., Chanda S.V. 1998. Modification of cell wall polysaccharides during cell elongation in Phaseolus vulgaris hypocotyls. Acta Physiol. Plant. 20: 15–18.
Bauer W.D., Talmadge K.W., Keegstra K., Albersheim P. 1973. The structure of plant cell walls II. The hemicellulose of the walls of suspension-cultured sycamore cells. Plant Physiol. 51: 174–187.
Brett C., Waldron K. 1991. In Physiology and Biochemistry of Plant Cell Walls. Topics in Plant Physiology II Eds. Black M., Chapman J. Unwin Hyman London Topics in Plant Physiology.
Chanda S.V., Bapodara C., Singh Y.D. 1995. Degradation of xyloglucan and pectic polysaccharides during cell elongation in Phaseolus vulgaris hypocotyls. Acta Plant. Physiol. 17: 349–356.
Doddemma H. and Telkamp G.P. 1979. Uptake of nitrate by mutants of Arabidopsis thaliana, disturbed in uptake of reduction of nitrate. II. Kinetics. Physiol. Plant. 45: 332–338.
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956. Colometric method for determination of sugars and related substances. Anal. Chem. 28:350–356.
Fry S.C. 1989. The structure and functions of xyloglucan. J. Exp. Bot. 40: 1–11.
Gokani S.J., Thaker V.S. 2000. Physiological and biochemical changes associated with cotton fiber development. VIII. Wall components. Acta Physiol. Plant. 22: 403–408.
Hayashi T. 1989. Xyloglucan in the primary cell wall. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40: 139–168.
Hoson T., Masuda Y., Sone Y., Misaki A. 1991. Xyloglucan antibodies inhibit auxin-induced elongation and cell wall loosening of azuki bean epicotyls but not of oat coleoptiles. Plant Physiol. 96: 551–557.
Hoson T. 1990. Effect of auxin on autolysis of cell walls in azuki bean epicotyls. Plant Cell Physiol. 31: 281–287.
Inouhe M., Yamamoto R., Masuda Y. 1984. Auxin induced changes in the molecular weight distribution of cell wall xyloglucans in Avena coleoptiles. Plant Cell Physiol. 25: 1341–1351.
Katsu N, Kamisaka S. 1983. Quantitative and qualitative changes in cell wall polysaccharides in relation to growth and cell wall loosening: Lactuca sativa hypocotyls. Physiol. Plant. 58: 33–40.
Kooiman P. 1960. A method for the detrmination of amyloid in plant seeds. Recl. Trav. Chim. Pays. Bas. 79: 675–678.
Labavitch J.M., Ray P.M. 1974. Relationship between promotion of xyloglucan metabolism and induction of elongation by indoleacetic acid. Plant Physiol. 54: 499–502.
McNeil M., Darvill A.G., Fry S..C., Albersheim P. 1984. Structure and function of the primary cell walls of plants. Annu. Rev. Biochem. 53: 625–663.
McQueen_Mason S. 1997. Plant cell walls and the control of growth. Biochem. Soc. Trans. 25: 204–214.
Nishitani K., Masuda Y. 1981. Auxin induced changes in the cell wall structure: change in the sugar composition intrinsic viscosity and molecular weight distribution of matrix polysaccharides of the epicotyl cell wall of Vigna angularis. Physiol. Plant. 52: 482–494.
Nishitani K., Masuda Y. 1983. Auxin induced changes in the cell wall xyloglucans: effect of auxin on the two different subfractions of xyloglucan in the epicotyl cell wall of Vigna angularis. Plant Cell Physiol. 24: 345–355.
Roberts K. 1990. Structures at the plant surface. Curr. Opinion Cell Biol. 2: 920–928.
Selvendran R.R., Du Pont M.S. 1984. In: Developments in food analysis techniques — 3, Ed. R.D. King pp 1–68 London and New York, Elsevier Applied Science Publishers
Taiz L. 1984. Plant cell expansion:relation of cell wall mechanical properties. Annu. Rev. Plant Physiol. 35: 585–657.
Terry M.E., Jones R.I., Bonner B.A. 1981. Soluble cell wall polysaccharides from pea stems by centrifugation. I. Effect of auxin. Plant Physiol. 68: 531–537.
Vincken J.P., York W.S., Beldman G., Voragen G.J. 1997. Two general branching pattern of xyloglucan XXXYG and XXXYGG. Plant Physiol. 114: 9–13.
Wada S. and Ray P.M. 1978. Matrix polysaccharides of oat coleoptile cell walls. Phytochemistry 17: 923–931.
Wakabayashi K., Hoson T., Kamisaka S. 1997. Changes in amounts and molecular mass distribution of cell wall polysaccharides of wheat (Triticum aestivum L.) coleoptiles under water stress. Plant Physiol. 151: 33–40.
Whitney S.E.C., Gothard M.G.E., Mitchell J.T., Gidley M.J. 1999. Roles of cellulose and xyloglucan in determining the mechanical properties of primary plant cell walls. Plant Physiol 121: 657–664.
Zhong H., Lauchli A. 1993. Changes of cell wall composition and polymer size in primary roots of cotton seedlings under high salinity. J. Exp. Bot. 44: 773–778.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chanda, S.V. Evaluation of effectiveness of the methods for isolation of cell wall polysaccharides during cell elongation in Phaseolus vulgaris seedlings. Acta Physiol Plant 27, 371–378 (2005). https://doi.org/10.1007/s11738-005-0014-6
Issue Date:
DOI: https://doi.org/10.1007/s11738-005-0014-6