Abstract
The plasmalemma vesicles isolated from cucumber and maize roots were used to study the effect of Cu2+ and Cd2+ on the hydrolytic and proton pumping activities of ATPase. In vivo application of metal ions to the plant growth solutions resulted in stimulation of the proton transport in maize. In cucumber roots the action of metals was not the same: cadmium stimulated the H+ transport through plasmalemma whereas Cu2+ almost completely inhibited it. Copper ions decreased the hydrolytic activity of H+-ATPase in cucumber, without any effect on this activity in membranes isolated from maize roots. The effect of cadmium on the hydrolytic activities was opposite: ATP-hydrolysis activity in plasmalemma was not altered in cucumber, whereas in maize its stimulation was observed. The amount of accumulated metals was not the main reason of different influence of metals on H+-ATPase activity in tested plants. In in vitro experiments Cu2+ inhibited H+ transport in the cucumber, to a higher degree than Cd2+ and both metals did not change this H+-ATPase activity of plasmalemma isolated from corn roots. Cu2+ added into the incubation medium reduced the hydrolytic activity of ATPase in the plasma membrane isolated from cucumber as well as from corn roots. Cd2+ diminished the hydrolytic activity of ATPase in cucumber, and no effect of Cd2+ in the plasmalemma isolated from corn roots was found. Our results indicated different in vitro and in vivo action of both metals on H+-ATPase and different response of this enzyme to Cu2+ and Cd2+ in maize and cucumber.
Similar content being viewed by others
Abbreviations
- PM:
-
plasma membrane
- TCA:
-
trichloroacetic acid
- BTP:
-
1,3-bis [tris-(hydroxymethyl) methylamino] propane
- MES:
-
2-(N-morpholino) ethanesulphonic acid
- Tris:
-
2 amino-2-hydroxymethyl
References
Ames B.N. 1966. Assay of inorganic phosphate, total phosphate and phosphatases. Methods of Enzymology 8: 115–118.
Binzel M. L. 1995. NaCl- induced accumulation of tonoplast and plasma membrane H+-ATPase message in tomato. Physiol. Plant. 94: 722–728
Blum-Zandstra M., Van Hattum J., Koot H.T.M. 1992. Relationship of nitrate and tonoplast ATPase activity in vacuoles and tonoplast vesicles from lettuce. Plant Science 87: 133–141
Blumwald E., Aharon G.S., Apse M.P. 2000. Sodium transport in plant cells. BBA 1465: 140–151
Bradford M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principles of protein-dye binding, Anal. Biochem. 72: 248–254
Burzyński M. 2001. Influence of pH on Cd and Cu uptake, distribution and their effect on nitrate reductase activity in cucumber (Cucumis sativus L.) seedling roots. Acta Physiol.Plant. 23:201–206
Burzyński M., Buczek J. 1994. The influence of Cd, Pb, Cu and Ni on NO −3 uptake by cucumber seedlings. II. In vitro and in vivo effect of Cd, Cu, Pb and Ni on the plasmalemma ATPase and oxidoreductase from cucumber seedlings roots. Acta Physiol.Plant 16: 297–302
Carruthers A., Melchior D. 1986. How bilayer lipids affect membrane protein activity? Trends in Biochemical Science 11: 331–335
Chaoui A., Mazhoudi S., Ghorbal M.H., Ferjani E.E. 1997. Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.) Plant Sci. 127: 139–147
Demidchik V., Sokolik A., Yurin Vladimir. 1997. The effect of Cu2+ on ion transport system of the plant cell plasmalemma. Plant Physiol. 114: 1313–1325
De Vos C.H.R., Schat H., De Waal M.A.M., Vooijs R., Ernst W.H.O. 1991. Increased resistance to copper-induced damage of root cell plasmalemma in copper tolerant Silene cucubalus. Physiol. Plant. 82: 523–528
Fodor E., Szabo-Nagy A., Erdei L. 1995. The effect of cadmium on fluidity and H+ — ATPase activity of plasma membrane from sunflower and wheat roots. J. Plant Physiol. 147: 87–92
Gallagher S.R., Leonard R.T. 1982. Effect of vanadate, molybdate, and azide on membrane-associated and soluble phosphatase activities of corn roots. Plant Physiol. 70: 1335–1340
Gonzales A., Koren’kov V., Wagner G.J. 1999. A comparision of Zn, Mn, Cd, and Ca transport mechanism in oat root tonoplast vesicles. Physiol. Plant. 106: 203–209
Halliwell B. 1978. Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. FEBS Lett. 96: 238–242
Harisson S.I., Lepp N.W., Phipps N.A. 1979. Uptake of copper by excised roots. II. Copper desorption from the free space. Z. Pflanzenphysiol. 94: 27–34
Hernandez L. E., Cook D.T. 1997. Modification of the root plasma membrane lipid composition of cadmium-treated Pisum sativum. J.Exp.Bot. 48: 1375–1381
Johansson T., Olbe M., Sommarin M., Larsson C. 1995. Brij 58, a polyoxyethylene acyl ether, creates membrane vesicles of uniform sidedness. A new tool to obtain inside-out plasma membrane vesicles. The Plant Journal Technical Advance 7: 165–173
Kenedy C.D., Gonsalves F.A.N. 1987. The action of divalent zinc, cadmium, mercury, copper and lead on the trans-root potential and H+ efflux of excised roots. J.Exp.Bot. 38: 800–817
Kenedy C.D., Gonsalves F.A.N. 1989. The action of divalent Zn, Cd, Hg, Cu and Pb ions on the ATPase activity of plasma membrane fraction isolated from roots of Zea mays. Plant and Soil 117: 167–175
Kłobus G., Buczek J. 1995. The role of plasma membrane oxidoreductase activity in proton transport. J. Plant Physiol. 146: 103–107.
Larsson C., Moller I.M., Widell S. 1990. An introduction to the plant plasma membrane-its molecular composition and organization. In Larsson C., Moller I.M. (eds). The plant plasma membrane. Springer-Verlag, Berlin Heidelberg, New York, London, Paris, Tokyo, Hong Kong, pp 1–16
Luna C.M., Gonzales C.A., Trippi V.C. 1994. Oxidative damage caused by an excess of copper in oat leaves. Plant Cell Physiol. 35(1): 11–15
Morsomme P., Boutry M. 2000. The plant plasma membrane H+ — ATPase: structure, function and regulation. BBA 1465: 1–16
Niu X., Narasimhan L., Salzman A., Bressan A., Hasegawa P.M. 1993. NaCl regulation of plasma membrane H+-ATPase gene expression in a glycophyte and halophyte. Plant Physiol. 103: 713–718
Ouariti O., Boussama N., Zarrouk M., Cherif A., Ghorbal M.H. 1997. Cadmium and copper-induced changes in tomato membrane lipids. Phytochem. 45: 1343–1350
Ros R., Cook D.T., Burden R. S., James C. 1990. Effect of herbicide MCPA, and the heavy metals, cadmium and nickel on the lipid composition, Mg2+ — ATPase activity and fluidity of plasma membranes from rice, Oryza sativa (cv. Bahia) shoots. J.Exp.Bot. 41: 457–462
Ros R., Cook D.T., Martinez-Cortina C., Picazo I. 1992a. Nickel and cadmium-related changes in growth, plasma membrane lipid composition, ATPase hydrolytic activity and proton-pumping of rice (Oryza sativa L cv. Bahia) shoots. J.Exp.Bot. 43: 1475–1481
Ros R., Morales A., Segura J., Picazo I. 1992b. In vivo and in vitro effects of nickel and cadmium on the plasmalemma ATPase from rice (Oryza sativa L.) shoots and roots. Plant Sci. 83: 1–6
Salt D.E., Wagner G.J. 1993. Cadmium transport across tonoplast of vesicles from oat roots. J.Biol.Chem. 268: 12297–12302
Sanderman H. 1978. Regulation of membrane enzymes by lipids. BBA 515: 209–237
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burzyński, M., Kolano, E. In vivo and in vitro effects of copper and cadmium on the plasma membrane H+-ATPase from cucumber (Cucumis sativus L.) and maize (Zea mays L.) roots. Acta Physiol Plant 25, 39–45 (2003). https://doi.org/10.1007/s11738-003-0034-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11738-003-0034-z