Abstract
The effects of the divalent metal ions Zn, Cd, Hg, Cu and Pb on the ATPase activity of a plasma membrane fraction isolated from roots ofZea mays have been investigated. When Mg-ions (3 mM), with or without K-ions (50mM) are included in the reaction medium, inhibition of ATPase activity was found in all cases, the relative order of the inhibitors over the concentration range 10 to 100μM, being Hg>>Cu∼Cd>Zn∼Pb. Below 1.0μM only Hg caused substantial inhibition. In the absence of Mg ions, Zn and to a lesser extent Cd, activated the enzyme up to a concentration of 1 mM, activity being further stimulated in the presence of K-ions (50mM). No activation of ATPase activity was found for Hg, Cu or Pb. It was concluded that Zn-ATP and Cd-ATP are both alternative substrates for the enzyme. Further experiments showed that both Km and Vmax for the substrates Zn-ATP and Cd-ATP are very much lower than for the usual substrate Mg-ATP.
These present results are discussed in relation to the known actions of these divalent cations on the trans-root potential and H-ion efflux in excised maize roots (Kennedy and Gonsalves, 1987).
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Abbreviations
- DTT:
-
DL-dithiothreitol
- EDTA:
-
Ethylene diamine tetra-actetic acid, disodium salt
- MES:
-
2-[N-morpholino] ethanesulphonic acid
- TES:
-
2([-hydroxyl-1, 1-bis(hydroxymethylethyl)] amino) ethanesulphonic acid
- TRIS:
-
2-amino-2-(hydroxymethyl) 1,3-propandiol
- TRP:
-
trans-root potential
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Kennedy, C.D., Gonsalves, F.A.N. The action of divalen Zn, Cd, Hg, Cu and Pb ions on the ATPase activity of a plasma membrane fraction isolated from roots ofZea mays . Plant Soil 117, 167–175 (1989). https://doi.org/10.1007/BF02220709
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DOI: https://doi.org/10.1007/BF02220709