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Characterisation of Solanum lycopersicoides Dun. root primordia culture with plant recovery

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Abstract

A liquid meristematic root primordia culture (RPC) of Solanum lycopersicoides Dun. based on persistent rhizogenesis in a modified Murashige and Skoog (1962) medium supplemented with NAA (15 mg·l−1) or 2,4-D (1 mg·l−1) was described. The meristematic clumps (2–3 mm in diameter) originating from NAA supplemented medium were capable of regenerating plants through the callus stage (up to 70 %). Efficient direct plant regeneration (up to 21 %) was possible from numerous single globular-shaped root primordia (RP) structures liberated from the parental aggregates in 2,4-D supplemented proliferation medium without NH4NO3 and with a 2.5 fold increase in KNO3. The RP converted into plantlets (artificial seedlings) on solid or liquid media without growth growth regulators through the unipolar followed by the mace-shaped bipolar structure stages. The use of apical shoot bud, root apices or root segments as a primary explants brought about RPC induction and plant regeneration. The plants derived from 2 years old culture were phenotypically identical to their parental S. lycopersicoides plants and possessed the same ploidy.

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Abbreviations

2,4-D:

dichlorophenoxy acetic acid

BA:

6-benzyladenine

NAA:

naphthalene acetic acid

RP:

root primordia

RPC:

root primordia culture

SMS:

modified Murashige and Skoog (1962) medium for Solanum lycopersicoides

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Authors and Affiliations

  1. Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska street 166, 02-787, Warsaw, Poland

    Wojciech Burza & Stefan Malepszy

  2. Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka street 19, 20-033, Lublin, Poland

    Ewa Dziadczyk

  3. Department of Biochemistry of Animals, Białystok University, Świerkowa street 20B, 15-950, Białystok, Poland

    Adam Tylicki

  4. Department of Plant Morphogenesis, Warsaw University, Miecznikowa street 1, 02-096, Warsaw, Poland

    Mieczysław Kuraś

Authors
  1. Wojciech Burza
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  2. Ewa Dziadczyk
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  3. Adam Tylicki
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  4. Mieczysław Kuraś
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  5. Stefan Malepszy
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Correspondence to Wojciech Burza.

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Burza, W., Dziadczyk, E., Tylicki, A. et al. Characterisation of Solanum lycopersicoides Dun. root primordia culture with plant recovery. Acta Physiol Plant 25, 385–394 (2003). https://doi.org/10.1007/s11738-003-0020-5

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  • DOI: https://doi.org/10.1007/s11738-003-0020-5

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