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Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, neutral invertase and soluble acid invertase in micropropagated sugarcane plants

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Abstract

The activities of sucrose-phosphate synthase (SPS), sucrose synthase (SUSY), neutral invertase (NI) and soluble acid invertase (SAI) regulates sucrose activity in sugarcane were studied. Micropropagated sugarcane plants were obtained from callus cultures of four Mexican commercially available sugarcane varieties characterized by differences in sugar production, and activities of SPS, SUSY, NI, SAI and concentrations of sucrose were monitored in the sugarcane stem. The results indicated that sucrose accumulation was positively and significantly related to an increase in activity of SPS and SUSY and negatively to a reduction in activity of SAI and NI (P<0.05). SPS explained most of the variations found for sucrose accumulation and least for NI. The relationship between activity of SPS, SUSY, NI and SAI in sugarcane stem was similar in each variety.

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Abbreviations

NI:

neutral invertase

SAI:

soluble acid invertase

SPS:

sucrose-phosphate synthase

SUSY:

sucrose synthase

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Authors and Affiliations

  1. Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Av. IPN 2508, CP 07360, México DF, México

    Federico A. Gutiérrez-Miceli, Martha A. Rodríguez-Mendiola, Neftalí Ochoa-Alejo, Ricardo Méndez-Salas, Luc Dendooven & Carlos Arias-Castro

  2. Laboratory of Vegetal Biotechnology, Instituto Tecnológico de Tuxtla-Gutierrez, Tuxtla-Gutierrez, México

    Federico A. Gutiérrez-Miceli

  3. Ciatej, Av. Normalistas No 800, Colinas de la Normal, CP 44270, Guadalajara, Jalisco, México

    Martha A. Rodríguez-Mendiola & Carlos Arias-Castro

  4. Unidad Irapuato del CINVESTAV, Km 9.6 Carr Irapuato-León, AP 629, CP 36500, México

    Neftalí Ochoa-Alejo

  5. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Campo Experimental Zacatepec, Zacatepec, Morelos, México

    Ricardo Méndez-Salas

  6. Departamento de Biotecnología y Bioingeniería, Cinvestav, Av IPN 2508, CP 07000, México DF

    Luc Dendooven

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  1. Federico A. Gutiérrez-Miceli
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  6. Carlos Arias-Castro
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Gutiérrez-Miceli, F.A., Rodríguez-Mendiola, M.A., Ochoa-Alejo, N. et al. Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, neutral invertase and soluble acid invertase in micropropagated sugarcane plants. Acta Physiol Plant 24, 441–446 (2002). https://doi.org/10.1007/s11738-002-0041-5

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  • DOI: https://doi.org/10.1007/s11738-002-0041-5

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