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The effect of sulphite on chlorophyll fluorescence and sucrose metabolism in poplar leaves

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Abstract

Sulphite at concentrations from 0.5 to 5.0 mM was supplied to illuminated, detached poplar (Populus deltoides Bartr. ex Marsh) leaves via the transpiration stream. Chlorophyll a fluorescence parameters, the contents of fructose-2,6-bisphosphate (Fru2,6BP) and starch, and extractable specific activity of sucrose-phosphate synthase (SPS), sucrose synthase (SuSy), acid invertase (AI), neutral invertase (NI), ATP-dependent fructose-6-phosphate 1-phosphotransferase (PFK) and pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP) were measured. Chlorophyll fluorescence parameters appeared to be unaffected by sulphite. Application of ≥ 1.0 mM sulphite led to an increase in the content of Fru2,6BP and starch. There was also a decline in the activity of SPS, NI and PFK. On the other hand, the influence of sulphite on the activity of AI and PFP was negligible. Specific activity of SuSy was inhibited by 1.0 and 2.5 mM but activated by 5.0 mM of sulphite. On the basis of the results obtained in the present study, we postulate that sulphite at concentrations ≥ 1.0 mM inhibits primarily sucrose synthesis, favours starch accumulation and has an indirect effect on the sucrolytic activities in poplar leaves.

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Abbreviations

AI:

acid invertase

Fv/Fm :

maximum photochemical efficiency of PS II

Fv/Fo :

efficiency of the photochemistry of PS II

Fru1,6BPase:

fructose-1,6-bisphosphatase

Fru2,6BP:

fructose-2,6-bisphosphate

LPI:

leaf plastochron index

NI:

neutral invertase

PEG:

polyethylene glycol

PFK:

ATP-dependent fructose-6-phosphate 1-phosphotransferase

PFP:

pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase

PVP:

polyvinylpyrrolidone

SPS:

sucrose-phosphate synthase

SuSy:

sucrose synthase

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Authors and Affiliations

  1. Polish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62-035, Kórnik, Poland

    Gabriela Lorenc-Plucińska, Anna Szadel, Andrzej Pluciński & Renata Matysiak

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  1. Gabriela Lorenc-Plucińska
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  2. Anna Szadel
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  3. Andrzej Pluciński
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  4. Renata Matysiak
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Lorenc-Plucińska, G., Szadel, A., Pluciński, A. et al. The effect of sulphite on chlorophyll fluorescence and sucrose metabolism in poplar leaves. Acta Physiol Plant 24, 123–129 (2002). https://doi.org/10.1007/s11738-002-0001-0

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  • DOI: https://doi.org/10.1007/s11738-002-0001-0

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