Abstract
A 2431-bp full-length cinnamate 4-hydroxylase gene, BoC4H, was cloned from Brassica oleracea L. var. acephala DC.. It contains 2 introns. Its mRNA is 1715 bp, encoding a deduced 481-amino-acid polypeptide with wide homologies to C4Hs from other plants. It possesses cytochrome P450 conserved domains and motifs such as the haem-iron binding motif, the E-R-R triad, the T-containing binding pocket motif and the hinge motif necessary for optimal orientation of the enzyme. It also has most of the canonical C4H/CYP73A5-featured substrate-recognition sites (SRSs) and active site residues. However, owing to a single-base deletion at C2242 and subsequent frame shift within the 3′ coding region as compared with C4H genes from Arabidopsis thaliana and other plants, BoC4H shows a 36-aa deletion/variation at its C-terminus and the SRS6 motif together with active site residues therein are absent. Thus BoC4H may be of no function or low activity. BoC4H is a membrane protein and is probably associated with the endoplasmic reticulum. Its secondary structure is dominated by alpha helices and random coils. The Swiss-Model could not predict its tertiary structure. B. oleracea contains a C4H gene family with at least 5 members.
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Translated from Acta Horticulturae Sinica, 2007, 34(4): 915–922 [译自 : 园艺学报]
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Chen, A., Li, J., Chai, Y. et al. Cloning and sequence analysis of a mutation-type cinnamate 4-hydroxylase gene from Brassica oleracea L. var. acephala DC.. Front. Agric. China 2, 456–462 (2008). https://doi.org/10.1007/s11703-008-0047-x
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DOI: https://doi.org/10.1007/s11703-008-0047-x