Abstract
Molecularly imprinted polymers (MIPs) are widely used in immunoassays as biomimetic receptors. MIPs are prepared by different polymerization methods and result in diverse performance in these assays. In this paper, MIPs were prepared via bulk polymerization, precipitation polymerization and a surface polymerization, which is newly developed by our group, to compare their performances in a biomimetic fluoroimmunosorbent assays. The imprinted polymers were prepared using the herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D) as the template, 4-vinylpyndine and ethylene glycol dimethacrylate as the functional and crosslinking monomer respectively by thermal polymerization. A novel fluorescent derivative of 2,4-D was synthesized from 9-anthracenemethanol to compete the binding sites of the MIPs with 2,4-D. The results show that the polymer coating provides the largest concentration range and the most facile format for the competitive immunoassay. The sandwich method can be used to produce very stable and reusable polymer coating which is expected to replace polymer particles in immunoassays.
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The raw data required to reproduce these findings are available to download from the website of European Polymer Journal in the form of supplementary information.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grants no. 21462043, 21365020 and 21565025) and the State Key Laboratory of Fine Chemicals (KF1617).
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AH: Data curation, writing-original draft. TM: Conceptualization, methodology, supervision, writing—reviewing and editing. MI: Methodology, supervision, writing—reviewing and editing. MW: Investigation, formal analysis. AY: Investigation, formal analysis.
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Hoji, A., Muhammad, T., Wubulikasimu, M. et al. A comparative study of biomimetic fluoroimmuno-assays based on 2,4-D imprinted polymers prepared by different polymerization techniques. Chem. Pap. 76, 3963–3970 (2022). https://doi.org/10.1007/s11696-022-02142-y
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DOI: https://doi.org/10.1007/s11696-022-02142-y