Abstract
Carotenoids, ergosterol and fatty acids are valuable compounds used in many industrial applications. The conventional sources of these compounds depend on weather conditions, season, geographical location and availability of agricultural land. Therefore, it is important to search for alternative sources and increase its availability for constantly increasing demand. Production of aforementioned compounds exploiting carotenogenic yeasts is promising due to their extraordinary capability to simultaneously produce carotenoids, ergosterol and fatty acids. The present study aimed to characterise the production properties of some lesser known carotenogenic yeasts, especially members of the orders Sporidiobolales and Cystofilobasidiales. The best fatty acid producing strains were S. metaroseus CCY 19-6-20 and C. macerans CCY 10-01-02, producing 4.9 g l−1 (42% of cell dry weight—CDW) and 4 g l−1 of medium (36% of CDW), respectively. The largest amounts of carotenoids, in the range 0.8–2.1 mg g−1 of CDW, were produced by strains R. mucilaginosa CCY 19-04-06, R. toruloides CCY 62-02-04, S. pararoseus CCY 19-09-06, S. metaroseus CCY 19-6-20, C. macerans CCY 10-01-02 and C. infirmominiatum CCY 1718-04. Surprisingly, R. mucilaginosa CCY 19-04-06 produced the highest amounts of carotenoids wherein lycopene represented more than 80% of total carotenoids.
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Acknowledgements
This research was funded by The Research Council of Norway, grant Lipofungi, number 268305, and supported by the project FCH-S-19-5983 of the Research Foundation of Brno University of Technology.
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IK: designed the study, the acquisition and analysis of data, and wrote the manuscript. DB: participated in the carotenoid analysis and discussion part of the study. MR: participated in fatty acids analysis and discussion part of the study. VB: participated on statistical analysis of the data. IM: participated in the design of the study and critically revised the manuscript. All authors approved the final submitted version.
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Kostovová, I., Byrtusová, D., Rapta, M. et al. The variability of carotenoid pigments and fatty acids produced by some yeasts within Sporidiobolales and Cystofilobasidiales. Chem. Pap. 75, 3353–3362 (2021). https://doi.org/10.1007/s11696-021-01567-1
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DOI: https://doi.org/10.1007/s11696-021-01567-1