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Changes of Short-Chain Fatty Acids and Their Receptors in an Obese Rat Model After Sleeve Gastrectomy

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Abstract 

Background

Short-chain fatty acids (SCFAs) and gut microbiota have health-related effects and are associated with a wide range of disorders. However, the changes of SCFAs and their receptors after sleeve gastrectomy (SG) remain unclear. This study aimed to examine changes of SCFAs and their receptors after SG in an obese rat model.

Methods

Thirty obese Sprague–Dawley rats eating a high-energy diet for 6 weeks were divided into three groups: sham-operated (SO) control, pair-fed (PF) control, and SG group. Six weeks after the surgery, metabolic parameters, SCFA levels in the blood and stool, mRNA and protein expression of SCFA receptors in the ileum and epididymal fat, and gut microbiota were examined.

Results

Metabolic parameters in the SG group were significantly improved compared with the SO group. Acetic acid levels in the blood and stool were significantly higher in the SG group than the PF group. The butyric acid level in the stool was also significantly higher in the SG group than in the PF group. In the ileum and epididymal fat, mRNA and protein expression of GPR41 was significantly higher in the SG group than in the other two groups, and mRNA and protein expression of GPR43 was significantly higher in the SG group than in the PF group. Increases in the genera Enterococcus, Lactobacillus, Lactococcus, and Clostridium were observed in the stool after SG.

Conclusions

SG may activate SCFA pathways through a change in gut microbiota.

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Acknowledgements

We would like to thank Mr. Taisei Ryuo, Ms. Akari Himeda, and Ms. Nozomi Ito for their technical assistance with the experiments.

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Authors and Affiliations

  1. Department of Gastroenterological and Pediatric Surgery, Faculty of Medicine, Oita University, Oita, Japan

    Atsuro Fujinaga, Masayuki Ohta, Yuichi Endo, Hiroaki Nakanuma, Masahiro Kawamura, Takahide Kawasaki, Takashi Masuda, Teijiro Hirashita & Masafumi Inomata

  2. Research Center for GLOBAL and LOCAL Infectious Diseases, Oita University, Oita, Japan

    Masayuki Ohta

  3. Department of Gastroenterology, Faculty of Medicine, Oita University, Oita, Japan

    Yuka Hirashita

  4. Department of Endocrinology, Metabolism, Rheumatology and Nephrology, Faculty of Medicine, Oita University, Oita, Japan

    Koro Gotoh

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  1. Atsuro Fujinaga
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  2. Masayuki Ohta
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  3. Yuichi Endo
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  4. Hiroaki Nakanuma
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  5. Masahiro Kawamura
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  7. Takahide Kawasaki
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  11. Masafumi Inomata
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Corresponding author

Correspondence to Atsuro Fujinaga.

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Ethical Approval

This study was approved by the Animal Committee of Oita University (#192002B).

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Key Points

• Acetic acid levels in blood and stool were significantly higher in the SG vs. PF.

• Butyric acid level in the stool was significantly higher in the SG vs. PF.

• GPR41 and GPR43 in the ileum and epididymal fat were significantly higher in the SG vs. PF.

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Fujinaga, A., Ohta, M., Endo, Y. et al. Changes of Short-Chain Fatty Acids and Their Receptors in an Obese Rat Model After Sleeve Gastrectomy. OBES SURG 32, 2649–2657 (2022). https://doi.org/10.1007/s11695-022-06130-9

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  • DOI: https://doi.org/10.1007/s11695-022-06130-9

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