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Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy

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Abstract

Background

We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum.

Methods

Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples.

Results

Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG.

Conclusion

This study proves that VSG suppresses hepatic glucose production.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No.2014R1A5A2010008), the research fund of Hanyang University (HY-2012-MC), and the Keimyung University Medical School Research Promoting Grant launched from 2012.

Conflict of Interest

Yunmee Lho, Carel W le Roux, Hyeon Soo Park, Gon Sup Kim, Keeyoun Jung, Geum-Sook Hwang, Youn Kyoung Seo, Tae Kyung Ha, and Eunyoung Ha have no conflicts of interest or other financial ties to disclose.

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Authors and Affiliations

  1. Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea

    Yunmee Lho & Eunyoung Ha

  2. Diabetes Complications Research Center, UCD Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland

    Carel W. le Roux

  3. Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Kyeongsang National University, Jinju, Republic of Korea

    Hyeon Soo Park & Gon Sup Kim

  4. KM Health Technology Research Group, Medical Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea

    Jeeyoun Jung

  5. Integrated Metabolomics Research Group of Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea

    Geum-Sook Hwang

  6. Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea

    Youn Kyoung Seo

  7. Department of Surgery, College of Medicine, Hanyang University, Seoul, Republic of Korea

    Tae Kyung Ha

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  1. Yunmee Lho
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  2. Carel W. le Roux
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Corresponding authors

Correspondence to Tae Kyung Ha or Eunyoung Ha.

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Lho, Y., le Roux, C.W., Park, H.S. et al. Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy. OBES SURG 25, 2002–2010 (2015). https://doi.org/10.1007/s11695-015-1636-4

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  • DOI: https://doi.org/10.1007/s11695-015-1636-4

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