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An alkali-extracted polysaccharide from Pleurotus eous and exploration of its antioxidant and immunomodulatory activities

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Abstract

The Pleurotus eous fruiting body was used to prepare an alkali-extracted polysaccharide (PEAP). The goal was to characterize PEAP’s physicochemical characteristics, structural characteristics, antioxidant capabilities, and immunological functions. PEAP had an average molecular weight of 156 kDa and was composed of glucose, galactose, rhamnose, xylose, and mannose. Both the FTIR and NMR spectra of PEAP showed distinctive polysaccharide bands. In addition to demonstrating significant scavenging activity against 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) (EC50 = 16.06 mg/mL), superoxide radicals (EC50 = 9.77 mg/mL), and 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) (EC50 = 16.21 mg/mL), PEAP also exhibited reducing power (EC50 = 9.71 mg/mL), chelating ability (EC50 = 7.89 mg/mL), and inhibition of β-carotene bleaching (EC50 = 2.06 mg/mL). PEAP was revealed to have an immunomodulatory effect as it increased the proliferative and phagocytic activity of RAW264.7 cells and caused concentration-dependent releases of nitric oxide (NO), interleukin 4 (IL-4), interleukin 12 (IL-12), interleukin 17 (IL-17), tumor necrosis factor (TNF-α), and interferon-gamma (IFN-γ) by macrophages. The results showed that PEAP might be a natural immunostimulant and antioxidant in functional foods or medicine.

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Acknowledgements

This work was supported by a Research Fellowship from Periyar University, India (Ref No. PU/AD-3/URF/2016). The Sophisticated Analytical Instruments Facility (SAIF) at CSIR-Central Food Technological Research Institute, Mysore, is acknowledged for molecular weight and monosaccharide composition analyses.

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Authors and Affiliations

  1. Department of Biochemistry, School of Biosciences, Periyar University, Salem, Tamil Nadu, 636011, India

    Amal Janardhanan, Sudha Govindan & Aswini Moorthy

  2. Department of Biochemistry, CSIR -Central Food Technological Research Institute, Mysuru, Karnataka, 570020, India

    Keelara Veerappa Harish Prashanth

  3. Post Graduate Department of Biochemistry, JSS College of Arts, Commerce and Science, Ooty Road, Mysore, Karnataka, 570025, India

    M. R. Savitha Prashanth

  4. Dhanvanthri Laboratory, Department of Sciences, Amrita School of Physical Science, Amrita Vishwa Vidyapeetham, Coimbatore, 641112, India

    Prasanna Ramani

  5. Center of Excellence in Advanced Materials & Green Technologies (CoE–AMGT), Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, 641112, India

    Prasanna Ramani

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  1. Amal Janardhanan
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  2. Sudha Govindan
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Correspondence to Sudha Govindan or M. R. Savitha Prashanth.

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Janardhanan, A., Govindan, S., Moorthy, A. et al. An alkali-extracted polysaccharide from Pleurotus eous and exploration of its antioxidant and immunomodulatory activities. Food Measure 18, 2489–2504 (2024). https://doi.org/10.1007/s11694-023-02318-4

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