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Effects of different extraction methods on the structural characterization and bioactivities of polysaccharides extracted from Polygonatum sibiricum

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Abstract

Polygonatum sibiricum is a traditional Chinese medicine with homology of medicine and food. Exploring different methods for extracting polysaccharides from Polygonatum sibiricum will provide broader insights into the study of its structural characteristics and biological activities. In this study, polysaccharides (PSPs) were extracted from by different methods with water (cold and hot), alcohol solutions (different concentrations) under ultrasound. The structure characterization of PSPs was mainly composed of Glc, Man and Gal with different molar ratio. HPLC data showed that the glycosidic bond of PSPs existed in α, β-D-configurations. Congo red experiment indicated that PSPs had similar triple helix structure. The PSPs extracted by different methods had different biological activities. PSP-5 had the best ability to inhibit AChE activity, and its mechanism was further explored. Results showed AChE inhibitory activity of PSP-5 was reversible, and the inhibitory mechanism was mixed type I competitive inhibition. Compared with the AChE inhibitors, PSP-5 had the potential to become an excellent AChE inhibitor.

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We declared that materials described in the manuscript, including all relevant raw data, will be freely available to any scientist wishing to use them for non-commercial purposes, without violating the confidentiality rules of the participants.

References

  1. C. Xiaowei, W. Wei, G. Hong, C. Hui, Z. Xiaofei, W. Haonan, W. Yumeng, Z. Xuelan, H. Chunchao, Review of Polygonatum sibiricum: a new natural cosmetic ingredient. Pharmazie 74(9), 513–519 (2019)

    CAS  PubMed  Google Scholar 

  2. X. Cui, S. Wang, H. Cao, H. Guo, Y. Li, F. Xu, M. Zheng, X. Xi, C. Han, A. Review, The bioactivities and pharmacological applications of Polygonatum sibiricum polysaccharides. Molecules (Basel, Switzerland) 23(5), 1170 (2018)

    Article  PubMed  Google Scholar 

  3. G. Wang, Y. Fu, J. Li, Y. Li, Q. Zhao, A. Hu, C. Xu, D. Shao, W. Chen, Aqueous extract of Polygonatum sibiricum ameliorates ethanol-induced mice liver injury via regulation of the Nrf2/ARE pathway. J. Food Biochem. 45(1), e13537 (2021)

    Article  CAS  PubMed  Google Scholar 

  4. D. Liu, W. Tang, C. Han, S. Nie, Advances in Polygonatum sibiricum polysaccharides: extraction, purification, structure, biosynthesis, and bioactivity. Front. Nutr. 9, 1074671 (2022)

    Article  PubMed  PubMed Central  Google Scholar 

  5. H. Gong, X. Gan, Y. Li, J. Chen, Y. Xu, S. Shi, T. Li, B. Li, H. Wang, S. Wang, Review on the genus Polygonatum polysaccharides: extraction, purification, structural characteristics and bioactivities. Int. J. Biol. Macromol. 229, 909–930 (2023)

    Article  CAS  PubMed  Google Scholar 

  6. W. Zhou, J. Hong, T. Liu, M. Li, H. Jin, X. Wang, Polygonatum polysaccharide regulates macrophage polarization and improves LPS-induced acute lung injury through TLR4-MAPK/NF-κB pathway. Can. Respir. J. 2022, 2686992 (2022)

    Article  PubMed  PubMed Central  Google Scholar 

  7. Y. Hu, Y. Tang, Z. Zhang, X. Guo, Z. Wu, Z. Li, H. Yu, W. Li, Recent advances in polysaccharides from the genus Polygonatum: Isolation, structures, bioactivities, and application. Food Hydrocolloids 140, 108634 (2023)

    Article  CAS  Google Scholar 

  8. L.-L. Su, X. Li, Z.-J. Guo, X.-Y. Xiao, P. Chen, J.-B. Zhang, C.-Q. Mao, D. Ji, J. Mao, B. Gao, T.-L. Lu, Effects of different steaming times on the composition, structure and immune activity of Polygonatum Polysaccharide. J. Ethnopharmacol. 310, 116351 (2023)

    Article  CAS  PubMed  Google Scholar 

  9. D. Qin, J. Xi, Flash extraction: an ultra-rapid technique for acquiring bioactive compounds from plant materials. Trends Food Sci. Technol. 112, 581–591 (2021)

    Article  CAS  Google Scholar 

  10. H. Zhang, X.T. Cai, Q.H. Tian, L.X. Xiao, Z. Zeng, X.T. Cai, J.Z. Yan, Q.Y. Li, Microwave-assisted degradation of polysaccharide from Polygonatum sibiricum and antioxidant activity. J. Food Sci. 84(4), 754–761 (2019)

    Article  CAS  PubMed  Google Scholar 

  11. S.S. Nadar, P. Rao, V.K. Rathod, Enzyme assisted extraction of biomolecules as an approach to novel extraction technology: a review. Food Res. Int. (Ottawa Ont) 108, 309–330 (2018)

    Article  CAS  Google Scholar 

  12. Y. Fan, X. Zhou, G. Huang, Preparation, structure, and properties of tea polysaccharide. Chem. Biol. Drug Des. 99(1), 75–82 (2022)

    Article  CAS  PubMed  Google Scholar 

  13. J. Li, G. Huang, Extraction, purification, separation, structure, derivatization and activities of polysaccharide from Chinese date. Process Biochem. 110, 231–242 (2021)

    Article  CAS  Google Scholar 

  14. B. Lin, G. Huang, Extraction, isolation, purification, derivatization, bioactivity, structure-activity relationship, and application of polysaccharides from White jellyfungus. Biotechnol. Bioeng. 119(6), 1359–1379 (2022)

    Article  CAS  PubMed  Google Scholar 

  15. J. Nai, C. Zhang, H. Shao, B. Li, H. Li, L. Gao, M. Dai, L. Zhu, H. Sheng, Extraction, structure, pharmacological activities and drug carrier applications of Angelica sinensis polysaccharide. Int. J. Biol. Macromol. 183, 2337–2353 (2021)

    Article  CAS  PubMed  Google Scholar 

  16. K. Sharma, M. Kumar, R. Waghmare, R. Suhag, O.P. Gupta, J.M. Lorenzo, S. Prakash, N. Radha, V. Rais, C. Sampathrajan, T. Thappa, A.A.S. Anitha, B.A. Sayed, M. Abdel-Wahab, R. Senapathy, A. Pandiselvam, S. Dey, R. Dhumal, J.F.K. Amarowicz, Moringa (Moringa oleifera Lam.) polysaccharides: extraction, characterization, bioactivities, and industrial application. Int. J. Biol. Macromol. 209, 763–778 (2022)

    Article  CAS  PubMed  Google Scholar 

  17. D. Canalejo, Z. Guadalupe, L. Martínez-Lapuente, B. Ayestarán, S. Pérez-Magariño, Optimization of a method to extract polysaccharides from white grape pomace by-products. Food Chem. 365, 130445 (2021)

    Article  CAS  PubMed  Google Scholar 

  18. S. Surin, S. You, P. Seesuriyachan, R. Muangrat, S. Wangtueai, A.R. Jambrak, S. Phongthai, K. Jantanasakulwong, T. Chaiyaso, Y. Phimolsiripol, Optimization of ultrasonic-assisted extraction of polysaccharides from purple glutinous rice bran (Oryza sativa L.) and their antioxidant activities. Sci. Rep. 10(1), 10410 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. P. Kumar, V. Kumar, Estimation of uronic acids using diverse approaches and monosaccharide composition of alkali soluble polysaccharide from Vitex negundo Linn. Carbohyd. Polym. 165, 205–212 (2017)

    Article  CAS  Google Scholar 

  20. Q.-C. Wang, X. Zhao, J.-H. Pu, X.-H. Luan, Influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of acidic, neutral and basic polysaccharides. Carbohyd. Polym. 143, 296–300 (2016)

    Article  CAS  Google Scholar 

  21. X. Yang, S. Wei, X. Lu, X. Qiao, J. Simal-Gandara, E. Capanoglu, Ł Woźniak, L. Zou, H. Cao, J. Xiao, X. Tang, N. Li, A neutral polysaccharide with a triple helix structure from ginger: characterization and immunomodulatory activity. Food Chem. 350, 129261 (2021)

    Article  CAS  PubMed  Google Scholar 

  22. M. Karaman, L. Janjušević, D. Jakovljević, F. Šibul, B. Pejin, Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract. Nat. Prod. Res. 33(10), 1522–1526 (2019)

    Article  CAS  PubMed  Google Scholar 

  23. R. Guo, J.A. Zhang, X. Liu, X. Li, X. Sun, Y. Kou, D. Li, Y. Liu, H. Zhang, Y. Wu, Pectic polysaccharides from Biluochun Tea: a comparative study in macromolecular characteristics, fine structures and radical scavenging activities in vitro. Int. J. Biol. Macromol. 195, 598–608 (2022)

    Article  CAS  PubMed  Google Scholar 

  24. C. Jing, Y. Yuan, Q. Tang, P. Zou, Y. Li, C. Zhang, Extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from Glycine soja. Int. J. Biol. Macromol. 103, 1207–1216 (2017)

    Article  CAS  PubMed  Google Scholar 

  25. P. Teerapongpisan, V. Suthiphasilp, T. Maneerat, R. Charoensup, T. Duangyod, R.J. Andersen, S. Laphookhieo, α-Glucosidase inhibitory and α-amylase inhibitory activities of compounds isolated from Uvaria rufa Blume. Nat. Prod. Res. 36(23), 6039–6043 (2022)

    Article  CAS  PubMed  Google Scholar 

  26. J.J. Nair, A.O. Aremu, J. van Staden, Isolation of narciprimine from Cyrtanthus contractus (Amaryllidaceae) and evaluation of its acetylcholinesterase inhibitory activity. J. Ethnopharmacol. 137(3), 1102–1106 (2011)

    Article  CAS  PubMed  Google Scholar 

  27. S. Kondo, T. Karasawa, A. Fukazawa, A. Koike, M. Tsutsui, S. Terada, Effects of a very high-carbohydrate diet and endurance exercise training on pancreatic amylase activity and intestinal glucose transporter content in rats. J. Nutr. Sci. Vitaminol. 68(2), 97–103 (2022)

    Article  CAS  PubMed  Google Scholar 

  28. G.D. Liyanaarachchi, J.K.R.R. Samarasekera, K.R.R. Mahanama, K.D.P. Hemalal, Tyrosinase, elastase, hyaluronidase, inhibitory and antioxidant activity of Sri Lankan medicinal plants for novel cosmeceuticals. Ind. Crops Prod. 111, 597–605 (2018)

    Article  CAS  Google Scholar 

  29. C. Papaneophytou, M.-E. Zervou, A. Theofanous, Optimization of a colorimetric assay to determine lactate dehydrogenase B activity using design of experiments. SLAS Discovery 26(3), 383–399 (2021)

    Article  CAS  PubMed  Google Scholar 

  30. H. Zhang, Y. Cao, L. Chen, J. Wang, Q. Tian, N. Wang, Z. Liu, J. Li, N. Wang, X. Wang, P. Sun, L. Wang, A polysaccharide from Polygonatum sibiricum attenuates amyloid-β-induced neurotoxicity in PC12 cells. Carbohyd. Polym. 117, 879–886 (2015)

    Article  CAS  Google Scholar 

  31. X. Li, Q. Chen, G. Liu, H. Xu, X. Zhang, Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities. Int. J. Biol. Macromol. 190, 730–738 (2021)

    Article  CAS  PubMed  Google Scholar 

  32. H.-Y. Ji, C. Liu, K.-Y. Dai, J. Yu, A.-J. Liu, Y.-F. Chen, The extraction, structure, and immunomodulation activities in vivo of polysaccharides from Salvia miltiorrhiza. Ind. Crops Prod. 173, 114085 (2021)

    Article  CAS  Google Scholar 

  33. R. Gnanasambandam, A. Proctor, Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. Food Chem. 68(3), 327–332 (2000)

    Article  CAS  Google Scholar 

  34. T. Zheng, D. Gu, X. Wang, X. Shen, L. Yan, W. Zhang, Y. Pu, C. Ge, J. Fan, Purification, characterization and immunomodulatory activity of polysaccharides from Leccinum crocipodium (Letellier.) Watliag. Int. J. Biol. Macromol. 148, 647–656 (2020)

    Article  CAS  PubMed  Google Scholar 

  35. Y. Wang, N. Liu, X. Xue, Q. Li, D. Sun, Z. Zhao, Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum. Int. J. Biol. Macromol. 160, 688–694 (2020)

    Article  CAS  PubMed  Google Scholar 

  36. S. Zhao, Z. Han, L. Yang, B. Hong, H. Zhu, Extraction, characterization and antioxidant activity evaluation of polysaccharides from Smilacina japonica. Int. J. Biol. Macromol. 151, 576–583 (2020)

    Article  CAS  PubMed  Google Scholar 

  37. Y. Su, L. Li, Structural characterization and antioxidant activity of polysaccharide from four auriculariales. Carbohyd. Polym. 229, 115407 (2020)

    Article  CAS  Google Scholar 

  38. X. Zhang, Q. Yu, H. Jiang, C. Ma, H.M. David Wang, J. Wang, W.-Y. Kang, A novel polysaccharide from Malus halliana Koehne with coagulant activity. Carbohydr. Res. 485, 107813 (2019)

    Article  CAS  PubMed  Google Scholar 

  39. M.E. Morales-Tovar, E.G. Ramos-Ramírez, J.A. Salazar-Montoya, Modeling and optimization of the parameters affecting extraction of the chan seed mucilage (Hyptis suaveolens (L.) Poit) by mechanical agitation (MA) and ultrasound-assisted extraction (UAE) in a multiple variables system. Food Bioproducts Process. 120, 166–178 (2020)

    Article  CAS  Google Scholar 

  40. X. Meng, C. Che, J. Zhang, Z. Gong, M. Si, G. Yang, L. Cao, J. Liu, Structural characterization and immunomodulating activities of polysaccharides from a newly collected wild Morchella sextelata. Int. J. Biol. Macromol. 129, 608–614 (2019)

    Article  CAS  PubMed  Google Scholar 

  41. B.C. Panda, S. Mondal, K.S.P. Devi, T.K. Maiti, S. Khatua, K. Acharya, S.S. Islam, Pectic polysaccharide from the green fruits of Momordica charantia (Karela): structural characterization and study of immunoenhancing and antioxidant properties. Carbohyd. Res. 401, 24–31 (2015)

    Article  CAS  Google Scholar 

  42. M.S. Kokoulin, I.N. Lizanov, L.A. Romanenko, I.V. Chikalovets, Structure of phosphorylated and sulfated polysaccharides from lipopolysaccharide of marine bacterium Marinicella litoralis KMM 3900T. Carbohyd. Res. 490, 107961 (2020)

    Article  CAS  Google Scholar 

  43. X. Liu, J. Bian, D. Li, C. Liu, S. Xu, G. Zhang, L. Zhang, P. Gao, Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L. Ind. Crops Prod. 129, 654–661 (2019)

    Article  CAS  Google Scholar 

  44. S. Tahmouzi, M.R. Salek Nejat, New infertility therapy effects of polysaccharides from Althaea officinalis leaf with emphasis on characterization, antioxidant and anti-pathogenic activity. Int. J. Biol. Macromol. 145, 777–787 (2020)

    Article  CAS  PubMed  Google Scholar 

  45. Y. Jia, X. Gao, Z. Xue, Y. Wang, Y. Lu, M. Zhang, P. Panichayupakaranant, H. Chen, Characterization, antioxidant activities, and inhibition on α-glucosidase activity of corn silk polysaccharides obtained by different extraction methods. Int. J. Biol. Macromol. 163, 1640–1648 (2020)

    Article  CAS  PubMed  Google Scholar 

  46. B.-H. Wang, J.-J. Cao, B. Zhang, H.-Q. Chen, Structural characterization, physicochemical properties and α-glucosidase inhibitory activity of polysaccharide from the fruits of wax apple. Carbohyd. Polym. 211, 227–236 (2019)

    Article  Google Scholar 

  47. C. Fang, G. Chen, J. Kan, Comparison on characterization and biological activities of Mentha haplocalyx polysaccharides at different solvent extractions. Int. J. Biol. Macromol. 154, 916–928 (2020)

    Article  CAS  PubMed  Google Scholar 

  48. W. Yang, Y. Fang, J. Liang, Q. Hu, Optimization of ultrasonic extraction of Flammulina velutipes polysaccharides and evaluation of its acetylcholinesterase inhibitory activity. Food Res. Int. 44(5), 1269–1275 (2011)

    Article  CAS  Google Scholar 

  49. R. Sajid ur, A. Saeed, G. Saddique, P. Ali Channar, F. Ali Larik, Q. Abbas, M. Hassan, H. Raza, T.A. Fattah, S.-Y. Seo, Synthesis of sulfadiazinyl acyl/aryl thiourea derivatives as calf intestinal alkaline phosphatase inhibitors, pharmacokinetic properties, lead optimization, Lineweaver-Burk plot evaluation and binding analysis. Bioorganic Med. Chem. 26(12), 3707–3715 (2018)

    Article  Google Scholar 

  50. N.V. Bhagavan, CHAPTER 6 - enzymes I: general properties, kinetics, and inhibition, in Medical biochemistry, 4th edn., ed. by N.V. Bhagavan (Academic Press, San Diego, 2002), pp.85–108

    Chapter  Google Scholar 

  51. C.G. Whiteley, Enzyme kinetics: partial and complete uncompetitive inhibition. Biochem. Educ. 28(3), 144–147 (2000)

    Article  CAS  PubMed  Google Scholar 

  52. S.S. Olesen, H. Krarup, J.L. Poulsen, J.H. Christensen, A.M. Drewes, Pancreas specific plasma amylase for assessment and diagnosis of chronic pancreatitis: new insights on an old topic. Pancreatology 18(4), S113 (2018)

    Article  Google Scholar 

  53. B. Jarrott, Tacrine: in vivo veritas. Pharmacol. Res. 116, 29–31 (2017)

    Article  PubMed  Google Scholar 

  54. N.R. Dunn, G.L. Pearce, S.A. Shakir, Adverse effects associated with the use of donepezil in general practice in England. J. Psychopharmacol. (Oxford, England) 14(4), 406–408 (2000)

    Article  CAS  Google Scholar 

  55. H. Kakegawa, H. Matsumoto, T. Satoh, Inhibitory effects of hydrangenol derivatives on the activation of hyaluronidase and their antiallergic activities. Planta Med. 54(5), 385–389 (1988)

    Article  CAS  PubMed  Google Scholar 

  56. T. Sundius, S.A. Brandán, Structural, harmonic force field and vibrational studies of cholinesterase inhibitor tacrine used for treatment of Alzheimer’s disease. Heliyon 9(6), e17280 (2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  57. H. Feng, J. Wu, P. Chen, J. Wang, Y. Deng, G. Zhu, J. Xian, L. Huang, W. Ouyang, MicroRNA-375-3p inhibitor suppresses angiotensin II-induced cardiomyocyte hypertrophy by promoting lactate dehydrogenase B expression. J. Cell. Physiol. 234(8), 14198–14209 (2019)

    Article  CAS  PubMed  Google Scholar 

  58. F. Khaleseh, H. Haghi-Aminjan, M. Samadi, Tacrine, reference module in biomedical sciences (Elsevier, Amsterdam, 2023)

    Google Scholar 

  59. S. Young, E. Chung, M.A. Chen, Cardiovascular complications of acetylcholinesterase inhibitors in patients with Alzheimer’s disease: a narrative review. Ann. Geriatric Med. Res. 25(3), 170–177 (2021)

    Article  Google Scholar 

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Acknowledgements

This work was supported by the Natural Science Foundation of China (Grant No. 82160786).

Funding

This work was supported by the Natural Science Foundation of China (Grant No. 82160786).

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Authors and Affiliations

  1. Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, 550001, China

    Mao-Hui Yang, Xin Zhou, Yan Yang & Hua-Guo Chen

  2. Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, 550001, China

    Mao-Hui Yang, Xin Zhou, Yan Yang & Hua-Guo Chen

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  1. Mao-Hui Yang
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  2. Xin Zhou
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  3. Yan Yang
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M-HY: Conceptualization, Investigation, Methodology, Writing-Original draft, Supervision, Writing-Reviewing and Editing. YY: Software, Methodology, Data curation. XZ: Supervision, Resources, Funding acquisition. H-GC: Supervision, Visualization, Supervision, Writing-Reviewing and Editing.

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Correspondence to Hua-Guo Chen.

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Yang, MH., Zhou, X., Yang, Y. et al. Effects of different extraction methods on the structural characterization and bioactivities of polysaccharides extracted from Polygonatum sibiricum. Food Measure 18, 940–954 (2024). https://doi.org/10.1007/s11694-023-02238-3

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