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Validation of high-performance liquid chromatography coupled with LTQ orbitrap mass spectrometry for analysis of acrylamide

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Abstract

Acrylamide is a food contaminant that forms through a natural chemical reaction between sugars and asparagine during high-temperature cooking processes. In this work an environmentally friendly and fast routine method for the analysis of acrylamide in Sudanese foods was successfully adapted. The method is based on sample extraction in water, purification with Carrez solutions and detection with high performance liquid chromatography–linear trap quadruple-Orbitrap-mass spectrometry (HPLC LTQ-Orbitrap MS). The performance of two analytical columns namely Kinetex C18 and Rezex ROA-organic acid was compared for acrylamide separation. The method was validated in term of linear range, limit of detection (LOD), limit of quantification (LOQ), precision (RSD%) and recovery. Good linearity (r2 > 0.9988) was achieved using standard addition method in the concentration range 0–200 µg kg− 1. The LOD is in the range from 3.24 to 4.66 µg kg− 1 and 2.47 to 3.94 µg kg− 1 for C18 and ROA columns, respectively. The precision of the method was ≤ 5.65% and 5.73% for C18 and ROA columns, respectively. Recoveries of acrylamide ranging from 86 to 98%, (n = 3) were obtained. It was successfully applied to the analysis of acrylamide in real food samples. Moreover, acrylamide precursors (free asparagine and reducing sugars) were analysed to show the relationship between their concentrations and acrylamide-forming potential.

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Acknowledgements

The authors are thankful to the Deanship of Scientific Research and under the supervision of the Scientific and Engineering Research Centre at Najran University for funding this work under the Research centers Funding program grant code (NU/RCP/SERC/12/1).

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Authors and Affiliations

  1. Central Laboratory, Ministry of Sciences & Technology, P.O. Box Office 7099, Khartoum, Sudan

    Mei Musa Ali Omar

  2. Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum, 11115, Sudan

    Abdalla Ahmed Elbashir

  3. Department of Chemistry, College of Science, King Faisal University, P.O.Box 400, Al-Ahsa, 31982, Saudi Arabia

    Abdalla Ahmed Elbashir

  4. Applied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany

    Oliver J. Schmitz

  5. Department of General Studies, Jubail Industrial College, PO Box 10099, Jubail Industrial City, 31961, Saudi Arabia

    Abobakr K. Ziyada

  6. Department of Chemical Engineering, College of Engineering, Najran University, P.O. Box 1988, Najran, 11001, Saudi Arabia

    Abdelbagi Osman

  7. Scientific and Engineering Research Centre, Najran University, P.O. Box 1988, Najran, 11001, Saudi Arabia

    Abdelbagi Osman

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  1. Mei Musa Ali Omar
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Omar, M.M.A., Elbashir, A.A., Schmitz, O.J. et al. Validation of high-performance liquid chromatography coupled with LTQ orbitrap mass spectrometry for analysis of acrylamide. Food Measure 18, 930–939 (2024). https://doi.org/10.1007/s11694-023-02223-w

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