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Characterization and microencapsulation of Lactobacillus plantarum FI 8595 cell free metabolites with enhanced antimicrobial property by powdered propolis

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Abstract

The concerns about synthetic preservatives on human health lead the food industry and scientists to seek natural agents from natural sustainable sources; including plants, animals, and microorganisms. In this study, it was aimed to characterise and enhance the bioactivity of microencapsulated cell free supernatant (CFS) of Lactobacillus plantarum FI 8595 by powdered propolis (PP) against fish spoilage bacteria and food-borne pathogens. CFS mainly consisted of n-hexadecanoic acid (27.44%) and 1-hexadecene (19.59%), while PP had primarily 2-furanmethanol (46.17%) and 2-ethyl hexanal (13.98%). Proteus mirabilis was the most resistant strain towards CFS, with inhibition zone of 8.43 mm. Addition of PP into CFS enhanced antimicrobial activity of lactobacilli CFS. Microencapsulated CFS with PP exerted higher inhibition zone (15 mm) than their pure form (9.33 mm) against Enterococcus faecalis. Staphylococcus aureus and Salmonella Paratyphi A exhibited stronger susceptibility versus pure and microencapsulated samples. Bacteriostatic effect of microencapsulated CFS was found at dose of 25 mg/ml for P. mirabilis, and 50 mg/ml for Pseudomonas luteola, E. faecalis, and P. damselae. All bacteria apart from P. luteola kept growing at 50 mg/ml dose of microencapsulated CFS, whereas microencapsulated CFS in combination with PP fully killed all bacteria at this dose. Results revealed that combinations of microencapsulated CFS and PP could be a good alternative to control microbial growth in food.

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Acknowledgements

The author would like to thank the academic staff of department of Seafood and Processing Technology, Faculty of Fisheries, Cukurova University (Adana, Turkey), who provided support in carrying out the work.

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  1. Department of Animal Feeding, Faculty of Agriculture, Cukurova University, Balcali, 01330, Adana, Turkey

    Aykut Burgut

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AB: Investigation, conceptualization, methodology, writing–original draft, review & editing.

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Correspondence to Aykut Burgut.

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Burgut, A. Characterization and microencapsulation of Lactobacillus plantarum FI 8595 cell free metabolites with enhanced antimicrobial property by powdered propolis. Food Measure 16, 4355–4363 (2022). https://doi.org/10.1007/s11694-022-01524-w

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