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Evaluation of the effects of extraction method, duration and harvesting time on qualitative and quantitative features of Medicago sativa

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Abstract

In recent years, numerous methods have been used to extract plants, and different procedures have been reported to produce various significant effects on extraction efficiency. Therefore, the aim of this study was to evaluate the impact of varied harvesting and experimental time on extraction efficiency. In this study, the impacts of different factors including extraction techniques, timing of each technique and harvesting time (second or third harvests) of Medicago sativa on its antioxidant properties, total phenol, flavonoids or carotenoids contents were examined. The ultrasound, microwave, as well as the percolation method were carried out separately for both second and third harvests. It was demonstrated that the effects of different harvesting times of M. sativa and its extraction time, as well as the interactions of each of these, on the contents of antioxidant compounds, total phenol, carotenoids, flavonoids were significant at 1% level (P < 0.01). However, the impact of the interactions of extraction methods and extraction time on antioxidants was significant only at 5% level (P < 0.05).It was indicated that the leaving the extract at inappropriate condition including light and oxygen in the percolation method (during long extraction) leads to lower antioxidant activity.

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References

  1. A.A. Izzo, E. Ernst, Interactions between herbal medicines and prescribed drugs. Drugs 61(15), 2163–2175 (2001)

    Article  CAS  Google Scholar 

  2. S. Hamedi, M. Honarvar, Beta vulgaris-A mini review of traditional uses in Iran, phytochemistry and pharmacology. Cur. Drug Discovery Technol. (2018)

  3. S. Paidari, S.A. Ibrahim. Potential application of gold nanoparticles in food packaging: a mini review. Gold Bull. 2021:1–6.

  4. J.C. Tilburt, T.J. Kaptchuk, Herbal medicine research and global health: an ethical analysis. Bull. World Health Organ. 86, 594–599 (2008)

    Article  Google Scholar 

  5. R. Hosseini, H. Ahari, P. Mahasti, S. Paidari, Measuring the migration of silver from silver nanocomposite polyethylene packaging based on (TiO 2) into penaeus semisulcatus using titration comparison with migration methods. Fish. Sci. 83(4), 649–659 (2017)

    Article  CAS  Google Scholar 

  6. S. Paidari, H. Ahari. The effects of nanosilver and nanoclay nanocomposites on shrimp (Penaeus semisulcatus) samples inoculated to food pathogens. J. Food Measure. Character. 1–12 (2021)

  7. Z. Ghorbani, N. Zamindar, S. Baghersad, S. Paidari, S.M. Jafari, L. Khazdooz. Evaluation of quality attributes of grated carrot packaged within polypropylene-clay nanocomposites. J. Food Measu. Charact. 1–12 (2021)

  8. R.D. Sewell. Rafieian-Kopaei M. The history and ups and downs of herbal medicines usage. J. HerbMed Pharmacol. 3 (2014)

  9. M. Gharachorloo, M. Honarvar, S. Mardani. Chemical compositions and antioxidant activity of Heracleum persicum essential oil. Braz. J. Pharmac. Sci. 53(3) (2017)

  10. S. Paidari, M. Goli, E. Anari, N. Haghdoust, Evaluation the effects of nanosilver composites synthesized using sol-gel method on inoculated Vibrio parahaemolyticus to pink shrimp. Acta Sci. Nutr. Health 3(2), 47–51 (2019)

    Google Scholar 

  11. Ahari H, Alinejad Dizaj M, Paidari S, Anvar A. The Effect of Gamma (γ) Irradiation to inactivate Escherichia coli in contaminated water. نشریه ایرانی سلامت حیوانات آبزی. 2(2), 88-96 (2016)

  12. E. Small, Adaptations to herbivory in alfalfa (Medicago sativa). Can. J. Bot. 74(6), 807–822 (1996)

    Article  Google Scholar 

  13. A. Anvar, S. Haghighat Kajavi, H. Ahari, A. Sharifan, A. Motallebi, S. Kakoolaki et al., Evaluation of the antibacterial effects of Ag-Tio2 nanoparticles and optimization of its migration to sturgeon caviar (Beluga). Iran. J. Fish. Sci. 18(4), 954–967 (2019)

    Google Scholar 

  14. R.A. Dixon, L.W. Sumner, Legume natural products: understanding and manipulating complex pathways for human and animal health. Plant Physiol. 131(3), 878–885 (2003)

    Article  CAS  Google Scholar 

  15. M. Jahadi, M. Ehsani, S. Paidari, Characterization of milk proteins in ultrafiltration permeate and their rejection coefficients. J. Food Biosci. Technol. 8(2), 49–54 (2018)

    Google Scholar 

  16. M. Rana, R. Katbamna, A. Padhya, A. Dudhrejiya, N. Jivani, N. Sheth, In vitro antioxidant and free radical scavenging studies of alcoholic extract of Medicago sativa L. Roman. J. Biol-Plant Biol. 55(1), 15–22 (2010)

    Google Scholar 

  17. H. Muraoka, N. Saigusa, K.N. Nasahara, H. Noda, J. Yoshino, T.M. Saitoh et al., Effects of seasonal and interannual variations in leaf photosynthesis and canopy leaf area index on gross primary production of a cool-temperate deciduous broadleaf forest in Takayama, Japan. J. Plant Res. 123(4), 563–576 (2010)

    Article  CAS  Google Scholar 

  18. M. Obanda, P.O. Owuor, R. Mang’oka, Changes in the chemical and sensory quality parameters of black tea due to variations of fermentation time and temperature. Food Chem. 75(4), 395–404 (2001)

    Article  CAS  Google Scholar 

  19. P.K. Mahanta, S. Baruah, Changes in pigments and phenolics and their relationship with black tea quality. J. Sci. Food Agric. 59(1), 21–26 (1992)

    Article  CAS  Google Scholar 

  20. G. Brink, M. Hall, G. Shewmaker, D. Undersander, N. Martin, R. Walgenbach, Changes in alfalfa yield and nutritive value within individual harvest periods. Agron. J. 102(4), 1274–1282 (2010)

    Article  Google Scholar 

  21. M.V. Bagavathiannan, R.C. Van Acker, The biology and ecology of feral alfalfa (Medicago sativa L.) and its implications for novel trait confinement in North America. Criti. Rev. Plant Sci. 28(12), 69–87 (2009)

    Article  Google Scholar 

  22. M. Bimakr, R.A. Rahman, F.S. Taip, A. Ganjloo, L.M. Salleh, J. Selamat et al., Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioproducts Process. 89(1), 67–72 (2011)

    Article  Google Scholar 

  23. K. Vilkhu, R. Mawson, L. Simons, D. Bates, Applications and opportunities for ultrasound assisted extraction in the food industry—a review. Innov. Food Sci. Emerg. Technol. 9(2), 161–169 (2008)

    Article  CAS  Google Scholar 

  24. B. Vongsak, P. Sithisarn, S. Mangmool, S. Thongpraditchote, Y. Wongkrajang, W. Gritsanapan, Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind. Crops Prod. 44, 566–571 (2013)

    Article  CAS  Google Scholar 

  25. M.P. Kähkönen, A.I. Hopia, H.J. Vuorela, J.-P. Rauha, K. Pihlaja, T.S. Kujala et al., Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem. 47(10), 3954–3962 (1999)

    Article  CAS  Google Scholar 

  26. Y. Velioglu, G. Mazza, L. Gao, B. Oomah, Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem. 46(10), 4113–4117 (1998)

    Article  CAS  Google Scholar 

  27. M.B. Hossain, N.P. Brunton, A. Patras, B. Tiwari, C. O’Donnell, A.B. Martin-Diana et al., Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology. Ultrason. Sonochem. 19(3), 582–90 (2012)

    Article  CAS  Google Scholar 

  28. F. Dahmoune, B. Nayak, K. Moussi, H. Remini, K. Madani, Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves. Food Chem. 166, 585–95 (2015)

    Article  CAS  Google Scholar 

  29. N. Azwanida, A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat Plants. 4(3), 3–8 (2015)

    Google Scholar 

  30. J. Mölgaard, H. Von Schenck, A.G. Olsson, Alfalfa seeds lower low density lipoprotein cholesterol and apolipoprotein B concentrations in patients with type II hyperlipoproteinemia. Atherosclerosis 65(1–2), 173–179 (1987)

    Article  Google Scholar 

  31. M. Hosseini, S. Asgary, S. Najafi, Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation. ARYA Atherosclerosis. 11(2), 133 (2015)

    PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Authors are thankful to Mr. Saeed Paidari for his helpful comments on preparation of this manuscript.

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Authors and Affiliations

  1. Department of Food Science and Technology, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran

    Zahra Nazari & Masoud Honarvar

  2. Department of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Marjan Dianat

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  1. Zahra Nazari
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  2. Masoud Honarvar
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  3. Marjan Dianat
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Correspondence to Masoud Honarvar.

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Nazari, Z., Honarvar, M. & Dianat, M. Evaluation of the effects of extraction method, duration and harvesting time on qualitative and quantitative features of Medicago sativa. Food Measure 15, 4868–4875 (2021). https://doi.org/10.1007/s11694-021-01056-9

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  • DOI: https://doi.org/10.1007/s11694-021-01056-9

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