Abstract
Pomegranate is a berry fruit that has attracted a lot of attention since, besides its appetizing taste, it confers important health benefits when consumed. Pomegranate derivatives such as seeds are also an excellent nutritional source of bioactive components and functional lipids. The present study aimed to evaluate the influence of microwave-assisted and ultrasound-assisted extractions, under different extraction parameters (temperature, extraction time, solvent to sample ratio) for the recovery of the highest amount of pomegranate seed oil. There were not significant differences between the obtained oil yields for microwave-assisted and ultrasound-assisted extractions (18.38 and 17.64%, respectively). For both methods, temperature and solvent to sample ratio exerted the main influence on pomegranate seed oil extraction yield, whereas extraction time only affected microwave-assisted extraction. Based on the obtained results and considering economic issues, the best treatment was using ultrasound-assisted extraction at 45 °C, 10 min and a solvent to sample ratio of 1:5 (g/mL), with an oil yield of 17.64%. Six fatty acids were identified by GC/MS, with punicic acid (> 75%) as the major residue in pomegranate seed oil. The extracted oil showed antioxidant values of 9.81 and 3.93 mg TE/g of seed, with 91.29 and 98.28% scavenging activity against DPPH and ABTS radicals, respectively. The oil also exhibited a 95.99% of lipid oxidation inhibition. Pomegranate seed is a potential source of health-promoting compounds, such as punicic acid, and natural antioxidants with a high radical scavenging activity.
Similar content being viewed by others
References
J.A. Ascacio-Valdés, J.J. Buenrostro-Figueroa, A. Aguilera-Carbo, A. Prado-Barragán, R. Rodríguez-Herrera, C.N. Aguilar, J. Med. Plants Res. 5(19), 4696–4703 (2011)
S. Smaoui, H.B. Hlima, A.C. Mtibaa, M. Fourati, I. Sellem, K. Elhadef, K. Ennouri, L. Mellouli, Meat Sci. 158, 107914 (2019)
M. Erkan, A.A. Kader, 14-Pomegranate (Punica granatum L.), in Postharvest Biology and Technology of Tropical and Subtropical Fruits. ed. by E.M. Yahia (Woodhead Publishing, Cambridge, 2011), pp. 287–313
A. Ali, Y. Chen, H. Liu, L. Yu, Z. Baloch, S. Khalid, J. Zhu, L. Chen, Int. J. Biol. Macromol. 129, 1120–1126 (2019)
E.P. Lansky, R.A. Newman, J. Ethnopharmacol. 109(2), 177–206 (2007)
Z. Amri, F. Zaouay, H. Lazreg-Aref, H. Soltana, A. Mneri, M. Mars, M. Hammami, Int. J. Biol. Macromol. 104, 274–280 (2017)
N. Kumar, S. Neerajand, Kumar, J. Pharm. Innov. 7(10), 71–81 (2018)
S. Đurđević, K. Šavikin, J. Živković, V. Böhm, T. Stanojković, A. Damjanović, S. Petrović, J. Supercrit. Fluids 133, 225–232 (2018)
M. Karimi, R. Sadeghi, J. Kokini, Trends Food Sci. Technol. 69, 59–73 (2017)
M. Kaufman, Z. Wiesman, J. Agric. Food Chem. 55(25), 10405–10413 (2007)
H.H. Orak, H. Yagar, S.S. Isbilir, Food Sci. Biotechnol. 21(2), 373–387 (2012)
S. Özgül-Yücel, J. Am. Oil Chem. Soc. 82(12), 893–897 (2005)
R. Holic, Y. Xu, K.M.P. Caldo, S.D. Singer, C.J. Field, R.J. Weselake, G. Chen, Appl. Microbiol. Biotechnol. 102(8), 3537–3549 (2018)
H.K. Çavdar, D.K. Yanık, U. Gök, F. Göğüş, Food Technol. Biotechnol. 55(1), 86–94 (2017)
A. Khoddami, T.H. Roberts, Lipid Technol. 27(2), 40–42 (2015)
A.-C. Schneider, P. Beguin, S. Bourez, J.W. Perfield II., E. Mignolet, C. Debier, Y.-J. Schneider, Y. Larondelle, PLoS ONE 7(3), e32824 (2012)
N.Z. Shaban, M.A.L. El-Kersh, F.H. El-Rashidy, N.H. Habashy, Food Chem. 141(3), 1587–1596 (2013)
V. Verardo, P. Garcia-Salas, E. Baldi, A. Segura-Carretero, A. Fernandez-Gutierrez, M.F. Caboni, Food Res. Int. 65, 445–452 (2014)
I. Pereira de Melo, E. Carvalho, J. Filho, J. Hum. Nutr. Food Sci. 2, 1024 (2014)
H. Abbasi, K. Rezaei, L. Rashidi, J. Am. Oil Chem. Soc. 85(1), 83–89 (2008)
G. Liu, X. Xu, Y. Gong, L. He, Y. Gao, Food Bioprod. Process. 90(3), 573–578 (2012)
M.H. Eikani, F. Golmohammad, S.S. Homami, Food Bioprod. Process. 90(1), 32–36 (2012)
Y. Tian, Z. Xu, B. Zheng, Y. Martin Lo, Ultrason. Sonochem. 20(1), 202–208 (2013)
D.B. Muñiz-Márquez, G.C. Martínez-Ávila, J.E. Wong-Paz, R. Belmares-Cerda, R. Rodríguez-Herrera, C.N. Aguilar, Ultrason. Sonochem. 20(5), 1149–1154 (2013)
E. Yabalak, B. Külekçi, A.M. Gizir, J. Environ. Sci. Heal. B 54(14), 1412–1422 (2019)
C. Castro-López, J.M. Ventura-Sobrevilla, M.D. González-Hernández, R. Rojas, J.A. Ascacio-Valdés, C.N. Aguilar, G.C.G. Martínez-Ávila, Food Chem. 237, 1139–1148 (2017)
Normas Mexicanas, NMX-F-607-NORMEX, México (2013). https://produccionindustrialdealimentos2.jimdofree.com/1-1-2-nmx-f-607-normex-2013/ (accessed 21 July 2019)
Normas Mexicanas, NMX-F-089-S, México (1978). https://www.colpos.mx/bancodenormas/nmexicanas/NMX-F-089-S-1978.PDF (accessed 18 July 2019)
Normas Mexicanas, NMX-F-090-S, México (1978). https://www.colpos.mx/bancodenormas/nmexicanas/NMX-F-090-S-1978.PDF (accessed 24 July 2019)
Normas Mexicanas, NMX-F-068-S, México (1980). https://sci-hub.tw/https://link.springer.com/article/10.1007/s11694-020-00452-x (accessed 20 July 2019)
N.P. Meléndez, V. Nevárez-Moorillón, R. Rodríguez-Herrera, J.C. Espinoza, C.N. Aguilar, Afr. J. Biochem. Res. 8(1), 14–18 (2014)
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Free Radic. Biol. Med. 26(9), 1231–1237 (1999)
G.C. Martínez-Ávila, A.F. Aguilera-Carbó, R. Rodríguez-Herrera, C.N. Aguilar, Ann. Microbiol. 62(3), 923–930 (2012)
G. López-García, L. López-Martínez, O. Dublán-García, R. Baeza-Jiménez, Rev. Mex. Ing. Quím. 16(3), 835–844 (2017)
A. Robledo, A. Aguilera-Carbó, R. Rodriguez, J.L. Martinez, Y. Garza, C.N. Aguilar, J. Ind. Microbiol. Biotechnol. 35(6), 507–513 (2008)
S. Dadashi, M. Mousazadeh, Z. Emam-Djomeh, S.M. Mousavi, Int. J. Adv. Biol. Biomed. Res. 4(4), 334–342 (2016)
M. Kýralan, M. Gölükcü, H. Tokgöz, J. Am. Oil Chem. Soc. 86(10), 985–990 (2009)
C.P. Van Nieuwenhove, A. Moyano, P. Castro-Gómez, J. Fontecha, G. Sáez, G. Zárate, P.L. Pizarro, LWT - Food Sci. Technol. 111, 401–407 (2019)
Z. Saeidi, B. Nasehi, H. Jooyandeh, J Food Sci Technol. 55(8), 3110–3118 (2018)
H. Bourekoua, R. Różyło, U. Gawlik-Dziki, L. Benatallah, M.N. Zidoune, D. Dziki, Int. J. Food Sci. Tech. 53(8), 1906–1913 (2018)
P. Aruna, B. Manohar, R.P. Singh, J. Food Process. Preserv. 42(5), e13609 (2018)
U.A. Fischer, A.V. Jaksch, R. Carle, D.R. Kammerer, Eur. Food Res. Technol. 237(2), 209–221 (2013)
J.M. Tirado-Gallegos, R. Baeza-Jiménez, J.A. Ascacio-Valdés, J.C. Bustillos-Rodriguez, J. Buenrostro-Figueroa, Pomegranate seeds as a potential source of punicic acid: extraction and nutraceutical benefits, in Practical Applications of Physical Chemistry in Food Science and Technology. ed. by C.N. Aguilar, J. Sandoval-Cortés, J.A. Ascacio-Valdés, A.K. Haghi (Apple Academic Press, Boca Raton, 2021), pp. 129–153
J. Zhong, Y. Wang, R. Yang, X. Liu, Q. Yang, X. Qin, Ind Crops Prod. 120, 1–10 (2018)
Y. Wang, J. You, Y. Yu, C. Qu, H. Zhang, L. Ding, H. Zhang, X. Li, Food Chem. 110(1), 161–167 (2008)
D.B. Nde, D. Boldor, C. Astete, Ind. Crops Prod. 65, 233–240 (2015)
R.P. Singh, K.N. Chidambara Murthy, G.K. Jayaprakasha, J. Agric. Food Chem. 50(1), 81–86 (2002)
M.L. Chávez-González, L.I. López-López, R. Rodríguez-Herrera, J.C. Contreras-Esquivel, C.N. Aguilar, Chem. Pap. 70(4), 412–417 (2016)
A.M. Goula, J. Food Eng. 117(4), 492–498 (2013)
Q.-A. Zhang, Z.-Q. Zhang, X.-F. Yue, X.-H. Fan, T. Li, S.-F. Chen, Food Chem. 116(2), 513–518 (2009)
Z. Hromádková, J. Kováčiková, A. Ebringerová, Ind. Crops Prod. 9(2), 101–109 (1999)
Z. Lou, H. Wang, M. Zhang, Z. Wang, J. Food Eng. 98(1), 13–18 (2010)
L. He, H. Xu, X. Liu, W. He, F. Yuan, Z. Hou, Y. Gao, Food Res. Int. 44(5), 1161–1167 (2011)
M. Durante, A. Montefusco, P.P. Marrese, M. Soccio, D. Pastore, G. Piro, G. Mita, M.S. Lenucci, J. Food Compos. Anal. 63, 65–72 (2017)
M. Bihamta, A. Hosseini, A. Ghorbani, M.T. Boroushaki, Avicenna J. Phytomed. 7(1), 46–53 (2017)
P. Aruna, D. Venkataramanamma, A.K. Singh, R.P. Singh, Compr. Rev. Food. Sci. Food Saf. 15(1), 16–27 (2016)
L. Hooper, L. Al-Khudairy, A.S. Abdelhamid, K. Rees, J.S. Brainard, T.J. Brown, S.M. Ajabnoor, A.T. O’Brien, L.E. Winstanley, D.H. Donaldson, F. Song, K.H. Deane, Cochrane Database Syst. Rev. 7(7), CD011094–CD011094 (2018)
F. Shahidi, P. Ambigaipalan, Annu. Rev. Food Sci. Technol. 9(1), 345–381 (2018)
J. Miranda, L. Aguirre, A. Fernández-Quintela, M.T. Macarulla, M.G. Martínez-Castaño, J. Ayo, E. Bilbao, M.P. Portillo, J. Agric. Food Chem. 61(21), 5089–5096 (2013)
Acknowledgements
Authors thank to National Council of Science and Technology (CONACYT), for the financial support received through the project CONACYT-PEI-252365. This project was performed out in the frame of the innovation stimulus program with ANTARES NETWORK S.A. DE C.V. Authors also thank CONACYT for the scholarship assigned to ERG in the PhD program in Food Science and Technology at the Research Center in Food and Development, A.C., Delicias, Chihuahua, México.
Funding
This research was supported by the CONACyT PEI Project 252365.
Author information
Authors and Affiliations
Contributions
All authors contributed to the study conception and design. Material preparation and data collection were performed by C-.M. and R-G. GC/MS analyses were performed by C-G. The first draft of the manuscript was written by R-G. Writing, review & editing were supported by all authors. B-F: conceptualization, formal analysis, funding acquisition, project administration, resources, writing-review and editing. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Rojo-Gutiérrez, E., Carrasco-Molinar, O., Tirado-Gallegos, J.M. et al. Evaluation of green extraction processes, lipid composition and antioxidant activity of pomegranate seed oil. Food Measure 15, 2098–2107 (2021). https://doi.org/10.1007/s11694-020-00804-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-020-00804-7