Skip to main content
SpringerLink
Log in

Optimization of antihypertensive and antioxidant hydrolysate extraction from rice bran proteins using ultrasound assisted enzymatic hydrolysis

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

In this study, antihypertensive and antioxidant protein extraction by means of ultrasound-assisted enzymatic hydrolysis was optimized in order to increase the added value of rice bran, which is a byproduct of rice milling. For this purpose, ultrasound treatment optimization was carried out with protein yield as response and solid/liquid ratio, time and power as independent factor using the response surface methodology (RSM). However, degree of hydrolysis (DH), ACE inhibitory activity, DPPH radical scavenging activity were selected as responses and solid/liquid ratio together with time as independent factors during alcalase pepsin and trypsin treatments. Three fractions depending on the molecular weight were separated by ultrafiltration. In enzyme treatments; the increase in solid/liquid ratio resulted in an increase in DH. On the other hand the impact of solid/liquid ratio and treatment time on antihypertensive and antioxidant properties varied. Differences of ACE inhibitory activity between ultrafiltrated protein fraction is not significant statistically. However, 5–10 kDa ultrafiltrated fraction is different for DPPH, hydroxy radical scavenging activity and trolox equivalent antioxidant capacity. Furthermore, the highest bioactivity values were obtained from 5–10 kDa ultrafiltrated fraction as 11.10% for DPPH, 6.20% for hydroxy radical scavenging activity and 8.19 µg trolox/mg sample for trolox equivalent antioxidant capacity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. G. Hu, S. Huang, S. Cao, Z. Ma, Effect of enrichment with hemicellulose from rice bran on chemical and functional properties of bread. Food Chem. 115(3), 839–842 (2009)

    CAS  Google Scholar 

  2. A. Moongngarm, N. Daomukda, S. Khumpika, Chemical compositions, phytochemicals, and antioxidant capacity of rice bran, rice bran layer, and rice germ. Apcbee Procedia. 2, 73–79 (2012)

    CAS  Google Scholar 

  3. M. Khoei, F. Chekin, The ultrasound-assisted aqueous extraction of rice bran oil. Food Chem. 194, 503–507 (2016)

    CAS  PubMed  Google Scholar 

  4. J. Wang, B. Sun, Y. Liu, H. Zhang, Optimisation of ultrasound-assisted enzymatic extraction of arabinoxylan from wheat bran. Food Chem. 150, 482–488 (2014)

    CAS  PubMed  Google Scholar 

  5. H. Tang, C.C.J. Wang, D. Blankschtein, R. Langer, An investigation of the role of cavitation in low-frequency ultrasound-mediated transdermal drug transport. Pharmaceutical Res. 19(8), 1160–1169 (2002)

    CAS  Google Scholar 

  6. F. Roy, J. Boye, B. Simpson, Bioactive proteins and peptides in pulse crops: pea, chickpea and lentil. Food Res. Int. 43(2), 432–442 (2010)

    CAS  Google Scholar 

  7. J.P. Maran, S. Manikandan, K. Thirugnanasambandham, C.V. Nivetha, R. Dinesh, Box-Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydr. Polym. 92(1), 604–611 (2013)

    Google Scholar 

  8. Z.Y. Park, D.H. Russell, Identification of individual proteins in complex protein mixtures by high-resolution, high-mass-accuracy MALDI TOF-mass spectrometry analysis of in-solution thermal denaturation/enzymatic digestion. Anal. Chem. 73(11), 2558–2564 (2001)

    CAS  PubMed  Google Scholar 

  9. J.Y. Je, P.J. Park, S.K. Kim, Antioxidant activity of a peptide isolated from Alaska pollack (Theragra chalcogramma) frame protein hydrolysate. Food Res. Int. 38(1), 45–50 (2005)

    CAS  Google Scholar 

  10. A.R. Jambrak, T.J. Mason, V. Lelas, L. Paniwnyk, Z. Herceg, Effect of ultrasound treatment on particle size and molecular weight of whey proteins. J. Food eng. 121, 15–23 (2014)

    CAS  Google Scholar 

  11. V. Yachmenev, B. Condon, T. Klasson, A. Lambert, Acceleration of the enzymatic hydrolysis of corn stover and sugar cane bagasse celluloses by low intensity uniform ultrasound. J. Biobased Mater. Bioenergy. 3(1), 25–31 (2009)

    CAS  Google Scholar 

  12. M. Sakakibara, D. Wang, R. Takahashi, K. Takahashi, S. Mori, Influence of ultrasound irradiation on hydrolysis of sucrose catalyzed by invertase. Enzyme Microb. Technol. 18(6), 444–448 (1996)

    CAS  Google Scholar 

  13. R. Liyanage, S. Minamino, Y. Nakamura, K.İ. Shimada, M. Sekikawa, K. Sasaki, Preparation method modulates hypocholesterolaemic responses of potato peptides. J. Funct. Foods 2(2), 118–125 (2010)

    CAS  Google Scholar 

  14. M. Palma, L.T. Taylor, R.M. Varela, S.J. Cutler, H.G. Cutler, Fractional extraction of compounds from grape seeds by supercritical fluid extraction and analysis for antimicrobial and agrochemical activities. J Agric. Food Chem. 47(12), 5044–5048 (1999)

    CAS  PubMed  Google Scholar 

  15. A.P. Adebiyi, A.O. Adebiyi, T. Ogawa, K. Muramoto, Purification and characterisation of antioxidative peptides from unfractionated rice bran protein hydrolysates. Int. J. Food Sci. Technol. 43(1), 35–43 (2008)

    CAS  Google Scholar 

  16. E.M. İşçimen, M. Hayta, Optimisation of ultrasound assisted extraction of rice bran proteins: effects on antioxidant and antiproliferative properties. Qual. Assur. Saf. Crops Foods. 10(2), 1–10 (2018). https://doi.org/10.3920/QAS2017.1186

    Article  Google Scholar 

  17. M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analy. Biochem. 72(1–2), 248–254 (1976)

    CAS  Google Scholar 

  18. T. Chittapalo, A. Noomhorm, Ultrasonic assisted alkali extraction of protein from defatted rice bran and properties of the protein concentrates. Int. J. food Sci. Technol. 44(9), 1843–1849 (2009)

    CAS  Google Scholar 

  19. D. Cushman, H. Cheung, Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. pharmacol. 20(7), 1637–1648 (1971)

    CAS  PubMed  Google Scholar 

  20. I. Orhan, M. Kartal, Q. Naz, A. Ejaz, G. Yilmaz, Y. Kan, B. Konuklugil, B. Sener, M.J. Choudhary, Antioxidant and anticholinesterase evaluation of selected Turkish Salvia species. Food Chem. 103(4), 1247–1254 (2007)

    CAS  Google Scholar 

  21. C.D. Cinq-Mars, E.C. Li-Chan, Optimizing angiotensin I-converting enzyme inhibitory activity of Pacific hake (Merluccius productus) fillet hydrolysate using response surface methodology and ultrafiltration. J. Agric. food Chem. 55(23), 9380–9388 (2007)

    CAS  PubMed  Google Scholar 

  22. K. Carbone, F. Mencarelli, Influence of short-term postharvest ozone treatments in nitrogen or air atmosphere on the metabolic response of white wine grapes. Food Bioprocess. Technol. 8(8), 1739–1749 (2015)

    CAS  Google Scholar 

  23. Y. Li, B. Jiang, T. Zhang, W. Mu, J. Liu, Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH). Food Chem. 106(2), 444–450 (2008)

    CAS  Google Scholar 

  24. F. Chemat, M.K. Khan, Applications of ultrasound in food technology: processing, preservation and extraction. Ultrasonics Sonochem. 18(4), 813–835 (2011)

    CAS  Google Scholar 

  25. W. Setyaningsih, E. Duros, M. Palma, C. Barroso, Optimization of the ultrasound-assisted extraction of melatonin from red rice (Oryza sativa) grains through a response surface methodology. Appl. Acoust. 103, 129–135 (2016)

    Google Scholar 

  26. S. Matsumoto, R.M. Varela, M. Palma, J.M. Molinillo, I.S. Lima, C.G. Barroso, Bio-guided optimization of the ultrasound-assisted extraction of compounds from Annona glabra L. leaves using the etiolated wheat coleoptile bioassay. Ultrasonics Sonochem. 21(4), 1578–1584 (2014)

    CAS  Google Scholar 

  27. P. Thamnarathip, K. Jangchud, S. Nitisinprasert, B. Vardhanabhuti, Identification of peptide molecular weight from rice bran protein hydrolysate with high antioxidant activity. J. Cereal Sci. 69, 329–335 (2016)

    CAS  Google Scholar 

  28. G.H. Li, M.R. Qu, J.Z. Wan, J.M. You, Antihypertensive effect of rice protein hydrolysate with in vitro angiotensin I-converting enzyme inhibitory activity in spontaneously hypertensive rats. Asia Pac. J. Clin. Nutr. 16(S1), 275–280 (2007)

    CAS  PubMed  Google Scholar 

  29. I. Thomson, W. Ladda, K. Supatcha, T. Chockchai, Pak. J. Nutr. 14(10), 647–652 (2015)

    Google Scholar 

  30. R. Horax, M.S. Vallecios, N. Hettiarachchy, L.F. Osorio, P. Chen, Solubility, functional properties, ACE-I inhibitory and DPPH scavenging activities of Alcalase hydrolysed soy protein hydrolysates. Int. J. Food Sci. Technol. 52(1), 196–204 (2017)

    CAS  Google Scholar 

  31. J. Capelo, R. Carreira, M. Diniz, L. Fernandes, M. Galesio, C. Lodeiro, Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques. Anal Chim Acta 650(2), 151–159 (2009)

    CAS  PubMed  Google Scholar 

  32. E.D. Marczak, H. Usui, H. Fujita, Y. Yang, M. Yokoo, A.W. Lipkowski, New antihypertensive peptides isolated from rapeseed. Peptides 24(6), 791–798 (2003)

    CAS  PubMed  Google Scholar 

  33. L. Switzar, M. Giera, W.M. Niessen, Protein digestion: an overview of the available techniques and recent developments. J. Proteome Res. 12(3), 1067–1077 (2013)

    CAS  PubMed  Google Scholar 

  34. L. Paniwnyk, H. Cai, S. Albu, T. Mason, R. Cole, The enhancement and scale up of the extraction of anti-oxidants from Rosmarinus officinalis using ultrasound. Ultrasonics Sonochem. 16(2), 287–292 (2009)

    CAS  Google Scholar 

  35. S. Jian, T. Wenyi, C. Wuyong, Ultrasound-accelerated enzymatic hydrolysis of solid leather waste. J. Clean. Prod. 16(5), 591–597 (2008)

    Google Scholar 

  36. K.X. Zhu, X.H. Sun, H.M. Zhou, Optimization of ultrasound-assisted extraction of defatted wheat germ proteins by reverse micelles. J. Cereal Sci. 50(2), 266–271 (2009)

    CAS  Google Scholar 

  37. N.P. Möller, K.E. Scholz-Ahrens, N. Roos, J. Schrezenmeir, Bioactive peptides and proteins from foods: indication for health effects. Eur. J. Nutr. 47(4), 171–182 (2008)

    PubMed  Google Scholar 

  38. A. Moure, H. Domínguez, J.C. Parajó, Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates. Process Biochem. 41(2), 447–456 (2006)

    CAS  Google Scholar 

  39. A.P. Adebiyi, A.O. Adebiyi, Y. Hasegawa, T. Ogawa, K. Muramoto, Isolation and characterization of protein fractions from deoiled rice bran. Eur. Food Res. Technol. 228(3), 391–401 (2009)

    CAS  Google Scholar 

Download references

Acknowledgements

This study is a part of research project (1130447) funded by the Scientific and Technological Research Council of Turkey (TUBITAK). The financial support provided by TUBITAK is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Engineering Faculty Food Engineering Department, Erciyes University, Kayseri, Turkey

    Mehmet Hayta, Büşra Benli, Elif Meltem İşçimen & Aslı Kaya

Authors
  1. Mehmet Hayta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Büşra Benli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elif Meltem İşçimen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aslı Kaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mehmet Hayta.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hayta, M., Benli, B., İşçimen, E.M. et al. Optimization of antihypertensive and antioxidant hydrolysate extraction from rice bran proteins using ultrasound assisted enzymatic hydrolysis. Food Measure 14, 2578–2589 (2020). https://doi.org/10.1007/s11694-020-00504-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-020-00504-2

Keywords

Search

Navigation