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Processed velvet antler: nutritional profile, in vitro antioxidant capacities, and alleviating symptoms of kidney-yang deficiency in mice

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Abstract

We present the nutritional composition, antioxidant activity, and pharmacological effects of three processed velvet antler products [antler powder (AP), antler slice (AS), and honey antler slice (HAS)] prepared in our laboratory. The nutrient profile of crude protein, fatty acids, amino acids, mineral elements, and nucleosides were established. The antioxidant activity was measured. The effects of three extracts on kidney-yang deficiency, induced by hydrocortisone, in mice were investigated. The nutrition composition of HAS was relatively low owing to its high moisture. AP, AS, and HAS extracts all exhibited concentration-dependent activity in DPPH*, ABTS, and FARP antioxidant assays. HAS had similar effect with AP and AS in improving oxidative stress in mice with kidney-yang deficiency, moreover, it could improve the serum cAMP level much significantly than the others. The processing without repeated heating might contribute in preserving more bioactive components in HAS, which could be the explanation of its excellent efficiency in relieving kidney-yang deficiency.

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Abbreviations

KYDS:

Kidney-yang deficiency syndrome

HPLC:

High performance liquid chromatography

GC:

Gas chromatography

TAA:

Total amino acids

TFA:

Total fatty acids

DPPH*:

2,2-Diphenyl-1-picrylhydrazyl

ABTS:

2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

FRAP:

Ferric ion reducing antioxidant power

TPTZ:

Tripyridyltriazine

TEAC:

Trolox equivalent antioxidant activity

ANOVA:

Analysis of variance

SOD:

Superoxide dismutase

MDA:

Malondialdehyde

GSH-PX:

Glutathione peroxidase

HE:

Hematoxylin eosin

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Acknowledgements

This work was supported by the National Key Research and Development Program of China [Grant Nos. 2018YFC1706605, 2018YFC1706604]; the Jilin Scientific and Technological Development Program [Grant Nos. 20170309002YY, 20170311027YY, 20180201076YY]; the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences [CAAS-ASTIP-2016-ISAPS].

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Authors and Affiliations

  1. Insitute of Special Animal and Plant Sciences of CAAS, 4889 Juye Street, Changchun, 130112, Jilin, People’s Republic of China

    Chang Liu, Zhiman Li, Chunai Jin, Lei Zhang, Songxin Liu, Ruize Gong, Zeshuai Wang & Yinshi Sun

  2. Jilin Agricultural University, 2888 Xincheng Street, Changchun, 130118, Jilin, People’s Republic of China

    Yunshi Xia

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  1. Chang Liu
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Correspondence to Yinshi Sun.

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Liu, C., Li, Z., Jin, C. et al. Processed velvet antler: nutritional profile, in vitro antioxidant capacities, and alleviating symptoms of kidney-yang deficiency in mice. Food Measure 14, 2163–2175 (2020). https://doi.org/10.1007/s11694-020-00463-8

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