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Fabrication of penicillin G portable microbiological test plate for on-farm antibiotic residues in milk

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Abstract

Antibiotic residues in animal-derived food products such as milk pose a potential health risk to consumers. A portable antimicrobial screening test plate was fabricated particularly for on-farm testing of milk. A cold-water soluble compound gelling agent composed of guar gum (GG) and konjac glucomannan (KG) was prepared and its properties were optimized for enhancing performance of the test plate. A ratio of 4:6 (GG:KG) showed improved gel strength, cohesiveness, transmittance, and water diffusion of the compound gel. Microbial colony number and size were also increased. The test plate displayed a minimum inhibition concentration of 4 µg/L penicillin G and clearer zone of inhibition. Detection time was also significantly reduced to approximately 9–15 h compared to the conventional agar plate method. The plastic film test plate with optimized compound gel demonstrated tremendous potential for detection of antibiotics in milk.

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Funding

We gratefully acknowledge the financial support from Jilin Provincial Science and Technology Development Plan under the International Science and Technology Cooperation Project: Study on Rapid detection of Antibiotics in Milk.

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Authors and Affiliations

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Moeketsi Ntakatsane, Ping Chen, Jingsheng Liu, Linlin Xu & Na Dong

  2. Department of Animal Science, Faculty of Agriculture, National University of Lesotho, Roma 180, Maseru, Lesotho

    Moeketsi Ntakatsane, Poloko Mosebi, George Adoko & Puleng Matebesi

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  1. Moeketsi Ntakatsane
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  2. Ping Chen
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  3. Jingsheng Liu
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Correspondence to Ping Chen.

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Ntakatsane, M., Chen, P., Liu, J. et al. Fabrication of penicillin G portable microbiological test plate for on-farm antibiotic residues in milk. Food Measure 14, 2087–2098 (2020). https://doi.org/10.1007/s11694-020-00456-7

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  • DOI: https://doi.org/10.1007/s11694-020-00456-7

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