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Physicochemical properties of caprine and commercial bovine whey protein concentrate

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Abstract

Whey protein from caprine milk, a by-product of cheese manufacturing, was recovered by ultrafiltration technology and dried by lyophilization. A caprine whey protein concentrate (WPCc) with 64.60% of protein content was prepared. The physicochemical properties of WPCc samples under different solutions (water or 0.1 M NaCl aqueous solution) and thermal conditions (25 °C and treatment at 90 °C for 30 min) were evaluated and compared with commercial bovine whey protein concentrate (WPCb). The chemical composition and the amino acid profiles were also determined. Both WPCc and WPCb showed high solubility in 0.1 M NaCl (almost 100%) and low solubility (38%) under heat treatment and at pH 5 (close to the isoelectric point). WPCc had a greater content of free sulfhydryl groups and higher positive and negative zeta potentials at extreme pH values than WPCb; however, the surface hydrophobicity of WPCc was lower. With respect to bovine whey protein concentrate, the caprine whey proteins presented higher amounts of basic amino acid and also of isoleucine, methionine, glycine and histidine. Results obtained in this work indicated that the caprine whey protein concentrate presents good and similar physicochemical properties to those observed for bovine whey protein concentrate and that it could be used for the same food applications.

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Acknowledgements

Financial support provided by the CICyT-UNSE (Project 23/A160) and PICT-O/2012 N° 0002 (ANPCyT) and also the fellowship of PhD Carolina Anabel Ayunta from CONICET (Argentina) are gratefully acknowledged.

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Authors and Affiliations

  1. CIBAAL (UNSE-CONICET), Centro de Investigación en Biofísica Aplicada y Alimentos, Univ. Nacional de Santiago del Estero, Ruta Nacional 9 km 1.125 Villa El Zanjón (CP4206), Santiago del Estero, Argentina

    Carolina A. Ayunta, Claudia M. Quinzio & Laura B. Iturriaga

  2. CIDCA (UNLP-CIC-CONICET), Facultad de Ciencias Exactas, Univ. Nacional de La Plata, Calle 47 y 116, La Plata, Buenos Aires, Argentina

    María C. Puppo

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  1. Carolina A. Ayunta
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  2. Claudia M. Quinzio
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Correspondence to Laura B. Iturriaga.

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Ayunta, C.A., Quinzio, C.M., Puppo, M.C. et al. Physicochemical properties of caprine and commercial bovine whey protein concentrate. Food Measure 13, 2729–2739 (2019). https://doi.org/10.1007/s11694-019-00194-5

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